{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T04:04:59Z","timestamp":1783915499306,"version":"3.55.0"},"reference-count":29,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,4,27]],"date-time":"2026-04-27T00:00:00Z","timestamp":1777248000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,4,27]],"date-time":"2026-04-27T00:00:00Z","timestamp":1777248000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Myeloproliferative neoplasms (MPNs), encompassing polycythemia vera (PV), essential thrombocythemia (ET), and primary myelofibrosis (PMF), are hematologic malignancies characterized by recurrent somatic mutations. Despite advances in next\u2010generation sequencing (NGS), the genetic basis of thrombotic risk and bone marrow (BM) fibrosis in MPNs remains unclear.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Patients diagnosed with classical MPN at Trakya University Faculty of Medicine were retrospectively analyzed (2018\u20132025). Mutation profiling was conducted on BM aspirates using a 78\u2010gene panel on the Illumina NextSeq platform, with clinically relevant variants (VAF \u2265\u20092%) interpreted via Qiagen Clinical Insight.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Among 91 patients with MPN, the most frequent mutations were JAK2 (71.4%), TET2 (23.1%), DNMT3A (15.4%), ASXL1 (11%), and splicing factor mutations (SFMs; 12%). Arterial events occurred in 45.1% of patients and were associated with age (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001), higher Charlson Comorbidity Index (CCI;\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001), ASXL1 (p:0.019), and SFMs (p:0.009). VTE occurred in 38.5% and was associated with JAK2 status and allele burden (p:0.004 and p:0.012 respectively), TET2 (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001), and SFMs (p:0.002); the JAK2\/TET2 co\u2010mutant subgroup had the highest risk of VTE risk in multivariable analysis (OR: 2.9, 95% CI: 1.4\u20135.7; p:0.002). Advanced BM fibrosis was associated with SFMs (p:0.018). Increased mutational burden\u00a0correlated with both venous and arterial thrombosis (p:0.021 and p:0.044, respectively). In survival analyses, SFMs (HR: 5.2; p:0.008), ASXL1 (HR: 3.8; p:0.030), and PMF diagnosis (HR: 3.8; p:0.045) were associated with inferior overall survival (OS).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Molecular profiling may provide clinically relevant thrombotic risk stratification in classical MPNs. JAK2\/TET2 co\u2010mutation was linked to VTE, while ASXL1 and SFMs were associated with adverse phenotypes including vascular events, BM fibrosis, and inferior OS.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/ijlh.70123","type":"journal-article","created":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T02:10:15Z","timestamp":1777342215000},"page":"848-855","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Clinical Impact of Next\u2010Generation Sequencing\u2013Detected Mutations on Thrombotic Events in Myeloproliferative Neoplasms"],"prefix":"10.1111","volume":"48","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6216-595X","authenticated-orcid":false,"given":"Ahmet","family":"Yigitbasi","sequence":"first","affiliation":[{"name":"Trakya University Faculty of Medicine, Department of Hematology  Edirne 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