{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T08:07:04Z","timestamp":1788941224948,"version":"build-2803163510"},"reference-count":28,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T00:00:00Z","timestamp":1777507200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T00:00:00Z","timestamp":1777507200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2026,10]]},"abstract":"ABSTRACT<\/jats:title>\n \n Background<\/jats:title>\n \n BCR\u2010ABL1\u2010negative myeloproliferative neoplasms (MPNs) often exhibit overlapping clinical and morphological features, making accurate subcategorization challenging. The h\u2010MICL (CD371) antigen, selectively expressed on leukemic stem cells (LSCs) and absent on CD34\n +<\/jats:sup>\n CD38\n \u2212<\/jats:sup>\n cells in normal or regenerating marrow, represents a potential diagnostic and prognostic marker.\n <\/jats:p>\n <\/jats:sec>\n \n Objectives<\/jats:title>\n \n This study aimed to evaluate the diagnostic utility of circulating CD34\n +<\/jats:sup>\n CD38\n \u2212<\/jats:sup>\n h\u2010MICL\n +<\/jats:sup>\n cells in subtyping BCR\u2010ABL1\u2010negative MPNs and to assess their correlation with the Dynamic International Prognostic Scoring System (DIPSS) score. Additionally, to identify a cut\u2010off value of CD34\n +<\/jats:sup>\n CD38\n \u2212<\/jats:sup>\n h\u2010MICL\n +<\/jats:sup>\n cells that can effectively differentiate PMF.\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n Fifty\u2010four patients with BCR\u2010ABL1\u2010negative MPNs were prospectively enrolled at a tertiary care center in North India over 18\u2009months. Peripheral blood was analyzed via flow cytometry to quantify CD34\n +<\/jats:sup>\n , CD34\n +<\/jats:sup>\n CD38\n +<\/jats:sup>\n , CD34\n +<\/jats:sup>\n CD38\n \u2212<\/jats:sup>\n , and CD34\n +<\/jats:sup>\n CD38\n \u2212<\/jats:sup>\n h\u2010MICL\n +<\/jats:sup>\n cell subsets.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n A significant increase in circulating CD34\n +<\/jats:sup>\n CD38\n \u2212<\/jats:sup>\n h\u2010MICL\n +<\/jats:sup>\n cells was observed in patients with overt MF (median 2.8%), prefibrotic MF (4.15%), and post\u2010PV\/ET MF (3%) compared to PV and ET (median 0%;\n p<\/jats:italic>\n \u2009<\u20090.001). A threshold of \u2265\u20090.9% CD34\n +<\/jats:sup>\n CD38\n \u2212<\/jats:sup>\n h\u2010MICL\n +<\/jats:sup>\n cells effectively identified MF cases with 88% sensitivity and 89% specificity. Moreover, a positive correlation was found between the percentage of CD34\n +<\/jats:sup>\n CD38\n \u2212<\/jats:sup>\n h\u2010MICL\n +<\/jats:sup>\n cells and DIPSS score (\n \u03c1<\/jats:italic>\n \u2009=\u20090.41,\n p<\/jats:italic>\n \u2009=\u20090.036), with every 1.72% increase in this cell population corresponding to a 1\u2010point rise in DIPSS score.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n \n Circulating CD34\n +<\/jats:sup>\n CD38\n \u2212<\/jats:sup>\n h\u2010MICL\n +<\/jats:sup>\n cells represent a promising biomarker; their quantification may aid in subclassification, particularly in distinguishing prefibrotic MF from ET, and may serve as a potential target for future therapeutic strategies. Further validation in larger cohorts is warranted.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/ijlh.70128","type":"journal-article","created":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T05:20:28Z","timestamp":1777612828000},"page":"1069-1077","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Role of Flow Cytometric Enumeration of Circulating\n hMICL<\/scp>\n (\n CD371<\/scp>\n ) Positive Leukemic Stem Cells in Diagnosis and Prognostication of\n BCR<\/scp>\n ::\n ABL1<\/scp>\n \u2010Negative Myeloproliferative Neoplasms"],"prefix":"10.1111","volume":"48","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2913-5171","authenticated-orcid":false,"given":"Gurleen","family":"Oberoi","sequence":"first","affiliation":[{"name":"Department of Hematology All India Institute of Medical Sciences New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rishi","family":"Dhawan","sequence":"additional","affiliation":[{"name":"Department of Hematology All India Institute of Medical Sciences New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8282-7842","authenticated-orcid":false,"given":"Jasmita","family":"Dass","sequence":"additional","affiliation":[{"name":"Department of Hematology All India Institute of Medical Sciences New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neha","family":"Ganju","sequence":"additional","affiliation":[{"name":"Department of Hematology All India Institute of Medical Sciences New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4793-6829","authenticated-orcid":false,"given":"Ganesh","family":"Viswanathan","sequence":"additional","affiliation":[{"name":"Department of Hematology All India Institute of Medical Sciences New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suman","family":"Lata","sequence":"additional","affiliation":[{"name":"Department of Hematology All India Institute of Medical Sciences New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9885-1625","authenticated-orcid":false,"given":"Mukul","family":"Agarwal","sequence":"additional","affiliation":[{"name":"Department of Hematology All India Institute of Medical Sciences New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pradeep Kumar","family":"Kumar","sequence":"additional","affiliation":[{"name":"Department of Hematology All India Institute of Medical Sciences New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tulika","family":"Seth","sequence":"additional","affiliation":[{"name":"Department of Hematology All India Institute of Medical Sciences New Delhi 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