{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T08:07:01Z","timestamp":1788941221049,"version":"build-2803163510"},"reference-count":34,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T00:00:00Z","timestamp":1778457600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T00:00:00Z","timestamp":1778457600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Acute myeloid leukemia (AML) is a clonal illness that is different in both medical and biological traits. Nonetheless, the genetic basis of this malignancy remains inadequately elucidated. MicroRNAs (miRNAs) are small noncoding RNAs that modify the expression of target mRNAs at both transcriptional and translational levels. In the past decade, miRNAs have evolved a unique mechanism in gene regulation, exhibiting varying expression throughout myeloid differentiation. This investigation examines the serum levels of miR\u2010383, miR\u2010381, and miR\u2010141 in AML and evaluates their diagnostic potential.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Serum samples from 110 AML patients and 110 normal individuals were evaluated using qRT\u2010PCR. Levels of expression were measured via the 2\n                      <jats:sup>\n                        \u2212\u0394\n                        <jats:italic>Ct<\/jats:italic>\n                      <\/jats:sup>\n                      technique and normalized by U6. Diagnostic performance was assessed by the ROC curve analysis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      MiR\u2010383 was significantly upregulated (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.0001), while miR\u2010381 and miR\u2010141 were downregulated (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.0002 and\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.0004, respectively) in AML patients. In addition, a considerable correlation was demonstrated between the expression levels of miR\u2010381 and subtype (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.02). In addition, the expression levels of miR\u2010141 and miR\u2010381 were positively correlated in AML patients (\n                      <jats:italic>r<\/jats:italic>\n                      \u2009=\u20090.99,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009\u2264\u20090.0001). The ROC analysis revealed strong potential of miR\u2010383 (AUC 0.87, 95% CI: 0.82\u20130.91), miR\u2010381 (AUC 0.71, 95% CI: 064\u20130.78), and miR\u2010141 (AUC 0.84, 95% CI: 0.79\u20130.89) as promising diagnostic biomarkers.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>These miRNAs display distinct serum expression levels in AML, with miR\u2010383, miR\u2010381, and miR\u2010141 demonstrating potential as diagnostic biomarkers, necessitating additional confirmation.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/ijlh.70144","type":"journal-article","created":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T03:04:13Z","timestamp":1778555053000},"page":"1086-1093","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Investigation of the Expression Levels of\n                    <scp>miR<\/scp>\n                    \u2010383,\n                    <scp>miR<\/scp>\n                    \u2010381, and\n                    <scp>miR<\/scp>\n                    \u2010141\n                    <scp>miRNAs<\/scp>\n                    as Diagnostic Biomarkers in Acute Myeloid Leukemia"],"prefix":"10.1111","volume":"48","author":[{"given":"Mayyadah Ali Ahmed","family":"AL\u2010Gburi","sequence":"first","affiliation":[{"name":"Department of Animal Biology, Faculty of Natural Science University of Tabriz  Tabriz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6970-6998","authenticated-orcid":false,"given":"Reza","family":"Safaralizadeh","sequence":"additional","affiliation":[{"name":"Department of Animal Biology, Faculty of Natural Science University of Tabriz  Tabriz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5890-4462","authenticated-orcid":false,"given":"Ali","family":"Rajabi","sequence":"additional","affiliation":[{"name":"Department of Animal Biology, Faculty of Natural Science University of Tabriz  Tabriz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammadali","family":"Hosseinpour Feizi","sequence":"additional","affiliation":[{"name":"Department of Animal Biology, Faculty of Natural Science University of Tabriz  Tabriz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,11]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.2021011304"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/ijlh.14211"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jfma.2022.10.007"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41375-023-01937-6"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2022.121869"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.2020007591"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2016.419"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12958-021-00857-3"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2022.955486"},{"issue":"1","key":"e_1_2_12_11_1","first-page":"164","article-title":"MiR\u2010381 Functions as a Tumor Suppressor in Gastric Cancer by Targeting ROCK2","volume":"12","author":"Xie Y.","year":"2019","journal-title":"International Journal of Clinical and Experimental Pathology"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbin.11071"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40001-020-00442-1"},{"issue":"12","key":"e_1_2_12_14_1","first-page":"11610","article-title":"Downregulation of miR\u2010381 Is Associated With Poor Prognosis in Papillary Thyroid Carcinoma","volume":"10","author":"Huang T.","year":"2017","journal-title":"International Journal of Clinical and Experimental Pathology"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2022.110354"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12943-022-01703-9"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12885-024-12794-6"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24065377"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12967-022-03456-x"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.genrep.2024.101966"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12885-020-07711-6"},{"issue":"5","key":"e_1_2_12_22_1","first-page":"6893","article-title":"MicroRNA\u2010383 Suppresses Cell Proliferation and Invasion in Colorectal Cancer by Directly Targeting Paired Box\u00a06","volume":"17","author":"Yan F.","year":"2018","journal-title":"Molecular Medicine Reports"},{"issue":"1","key":"e_1_2_12_23_1","first-page":"1184","article-title":"Upregulation of microRNA\u2010383 Inhibits the Proliferation, Migration and Invasion of Colon Cancer Cells","volume":"15","author":"Cui Y.","year":"2018","journal-title":"Oncology Letters"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10528-017-9793-x"},{"issue":"2","key":"e_1_2_12_25_1","first-page":"2066","article-title":"MicroRNA\u2010381 Suppresses Proliferation and Invasion of Prostate Cancer Cells Through Downregulation of the Androgen Receptor","volume":"18","author":"Rui X.","year":"2019","journal-title":"Oncology Letters"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2016.12.051"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.3390\/genes10040319"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.1159\/000438641"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.3390\/genes12020267"},{"key":"e_1_2_12_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-14088-3"},{"issue":"4","key":"e_1_2_12_31_1","first-page":"3994","article-title":"The microRNA\u2010200 Family Acts as an Oncogene in Colorectal Cancer by Inhibiting the Tumor Suppressor RASSF2","volume":"18","author":"Carter J. V.","year":"2019","journal-title":"Oncology Letters"},{"key":"e_1_2_12_32_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers15092527"},{"key":"e_1_2_12_33_1","doi-asserted-by":"publisher","DOI":"10.3390\/s21093183"},{"key":"e_1_2_12_34_1","doi-asserted-by":"publisher","DOI":"10.3390\/ncrna11050070"},{"key":"e_1_2_12_35_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2016-10-697698"}],"container-title":["International Journal of Laboratory Hematology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ijlh.70144","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/ijlh.70144","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ijlh.70144","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T07:37:06Z","timestamp":1788939426000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ijlh.70144"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5,11]]},"references-count":34,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2026,10]]}},"alternative-id":["10.1111\/ijlh.70144"],"URL":"https:\/\/doi.org\/10.1111\/ijlh.70144","archive":["Portico"],"relation":{},"ISSN":["1751-5521","1751-553X"],"issn-type":[{"value":"1751-5521","type":"print"},{"value":"1751-553X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5,11]]},"assertion":[{"value":"2025-12-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-06","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-11","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}