{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T03:39:57Z","timestamp":1792381197373,"version":"4.4.0"},"reference-count":25,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,6,7]],"date-time":"2026-06-07T00:00:00Z","timestamp":1780790400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,6,7]],"date-time":"2026-06-07T00:00:00Z","timestamp":1780790400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100002742","name":"Banaras Hindu University","doi-asserted-by":"publisher","award":["R\/Dev\/D\/IoE\/Seed Grant\u2010II\/2021\u201022\/40018"],"award-info":[{"award-number":["R\/Dev\/D\/IoE\/Seed Grant\u2010II\/2021\u201022\/40018"]}],"id":[{"id":"10.13039\/501100002742","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Tyrosine kinase inhibitors (TKIs) have transformed the treatment of chronic myeloid leukemia (CML); yet, diverse molecular responses and resistance persist. BCR::ABL1 kinase\u2010domain (TKD) mutations constitute just a fraction of this resistance, and the impact of additional somatic mutations on disease progression and early molecular response remains incompletely defined.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>This single\u2010centre cohort study analyzed 109 NGS\u2010tested patients with CML, comprising 44 with TKI\u2010resistant disease and 65 newly diagnosed patients. Targeted next\u2010generation sequencing using a 135\u2010gene myeloid panel was performed on 109 patients. An additional pilot subgroup of 30 TKI\u2010resistant patients underwent BCR::ABL1 kinase\u2010domain analysis by PCR\/Sanger sequencing and was analyzed separately. Molecular response was assessed using BCR::ABL1 transcript levels on the International Scale and interpreted according to ELN 2020 recommendations.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Somatic mutations were identified in 52.3% of TKI\u2010resistant and 29.2% of newly diagnosed patients. All Cohort 1 blast\u2010crisis patients were mutation\u2010positive, and several concurrent abnormalities were more common in Cohort 1 than in Cohort 2, indicating clonal complexity. In Cohort 2, MMR was achieved in 28\/39 (71.8%) mutation\u2010negative and 6\/13 (46.2%) mutation\u2010positive patients. Mutation\u2010positivity at baseline was associated with reduced MMR chances but not statistically significant (odds ratio 0.34; 95% confidence interval 0.09\u20131.23;\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.099). ASXL1 emerged as the most common non\u2010ABL1 mutation but was not statistically significant.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>In this Indian CML cohort, somatic mutations were prevalent in TKI\u2010resistant disease, linked to advanced phase and clonal complexity, and demonstrated a non\u2010significant trend toward lower early MMR at diagnosis, highlighting the importance of genomic testing in this context.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/ijlh.70165","type":"journal-article","created":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T01:04:42Z","timestamp":1780880682000},"page":"1141-1148","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Impact of Somatic Mutations on Treatment Response and Resistance in Chronic Myeloid Leukemia"],"prefix":"10.1111","volume":"48","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1494-3064","authenticated-orcid":false,"given":"Shruti","family":"Mishra","sequence":"first","affiliation":[{"name":"Bone Marrow Transplantation and Stem Cell Research Centre, Institute of Medical Sciences Banaras Hindu University  Varanasi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kailash","family":"Kumar","sequence":"additional","affiliation":[{"name":"Department of General Medicine, Institute of Medical Sciences Banaras Hindu University  Varanasi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dinesh","family":"Kumar","sequence":"additional","affiliation":[{"name":"Department of General Medicine, Institute of Medical Sciences Banaras Hindu University  Varanasi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashwin","family":"Kamak","sequence":"additional","affiliation":[{"name":"Department of General Medicine, Institute of Medical Sciences Banaras Hindu University  Varanasi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lovely","family":"Singh","sequence":"additional","affiliation":[{"name":"Department of General Medicine, Institute of Medical Sciences Banaras Hindu University  Varanasi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nilesh","family":"Kumar","sequence":"additional","affiliation":[{"name":"Department of General Medicine, Institute of Medical Sciences Banaras Hindu University  Varanasi India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,7]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.27443"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.12703\/r\/10\u201035"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41375\u2010023\u201002048\u2010y"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41375\u2010025\u201002664\u2010w"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.6004\/jnccn.2023.7335"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.6004\/jnccn.2024.0007"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41375-019-0512-y"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2018.200220"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1182\/bloodadvances.2019000943"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1055\/s00421757911"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s0027720042898"},{"issue":"2","key":"e_1_2_12_13_1","first-page":"135","article-title":"Multi Drug Resistant Chronic Myeloid Leukemia: Report of Two Cases and Review of Literature","volume":"12","author":"Rahman M. 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