{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T03:39:49Z","timestamp":1792381189588,"version":"4.4.0"},"reference-count":28,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T00:00:00Z","timestamp":1782432000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T00:00:00Z","timestamp":1782432000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>Centrifugation requirements differ between coagulation and clinical chemistry testing, potentially limiting workflow efficiency and total laboratory automation (TLA). Coagulation assays typically require standard centrifugation at 1500\u2009g for 15\u2009min, whereas chemistry samples use a rapid protocol at 2300\u2009g for 5\u2009min. This study evaluated the impact of rapid centrifugation on prothrombin time (PT), activated partial thromboplastin time (APTT), and D\u2010dimer results.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Blood samples were prospectively collected from volunteers in 3, 2, and pediatric 1\u2009mL citrate tubes and were processed using standard or rapid centrifugation. Testing was performed for PT, APTT, and D\u2010Dimer on Sysmex CN3000 and ACL\u2010TOP 750 analyzers, with residual platelet counts assessed.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      A total of 130 samples were analyzed (41 from 3\u2009mL tubes, 41 from 2\u2009mL tubes, and 39 from 1\u2009mL tubes). Rapid centrifugation produced significantly higher residual platelet counts than the standard protocol (39.2\u2009\u00d7\u200910\n                      <jats:sup>9<\/jats:sup>\n                      \/L vs. 18.8\u2009\u00d7\u200910\n                      <jats:sup>9<\/jats:sup>\n                      \/L,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.0001). Strong correlations (\n                      <jats:italic>r<\/jats:italic>\n                      \u2009&gt;\u20090.9) were observed for PT and APTT across analyzers, while D\u2010dimer results on ACL\u2010TOP 750 showed slightly lower correlation (\n                      <jats:italic>r<\/jats:italic>\n                      \u2009=\u20090.830). Bland\u2013Altman analysis demonstrated small mean biases (&lt;\u20091% for PT and APTT). D\u2010dimer biases from CN3000 and ACL\u2010TOP 750 were 1.06% and \u22122.70%, respectively, which remained within the predefined total allowable error. Residual platelets showed no impact on test results.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Rapid centrifugation at 2300\u2009g for 5\u2009min provides coagulation results comparable to the standard protocol and may facilitate integration of coagulation testing into TLA workflows.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/ijlh.70188","type":"journal-article","created":{"date-parts":[[2026,6,27]],"date-time":"2026-06-27T02:09:41Z","timestamp":1782526181000},"page":"1201-1210","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Rapid Centrifugation for Coagulation Testing: A Multianalyzer Validation Study Harmonizing Preanalytical Protocols for Total Laboratory Automation"],"prefix":"10.1111","volume":"48","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-1790-9841","authenticated-orcid":false,"given":"Orakan","family":"Limpornpugdee","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine, Faculty of Medicine Chulalongkorn University  Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jirapa","family":"Kaewkhruawan","sequence":"additional","affiliation":[{"name":"Division of Laboratory Medicine, King Chulalongkorn Memorial Hospital  Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pitchayaporn","family":"Riyagoon","sequence":"additional","affiliation":[{"name":"Division of Laboratory Medicine, King Chulalongkorn Memorial Hospital  Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Waroonkarn","family":"Laiklang","sequence":"additional","affiliation":[{"name":"Division of Laboratory Medicine, King Chulalongkorn Memorial Hospital  Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wararat","family":"Masalae","sequence":"additional","affiliation":[{"name":"Division of Laboratory Medicine, King Chulalongkorn Memorial Hospital  Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Surapat","family":"Tanticharoenkarn","sequence":"additional","affiliation":[{"name":"Division of Laboratory Medicine, King Chulalongkorn Memorial Hospital  Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kwanlada","family":"Chaiwong","sequence":"additional","affiliation":[{"name":"Division of Laboratory Medicine, King Chulalongkorn Memorial Hospital  Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Phongsapak","family":"Pipatnavakij","sequence":"additional","affiliation":[{"name":"Division of Laboratory Medicine, King Chulalongkorn Memorial Hospital  Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Parinda","family":"Limprasert","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Faculty of Medicine Chulalongkorn University  Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7043-8062","authenticated-orcid":false,"given":"Chutitorn","family":"Ketloy","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Faculty of Medicine Chulalongkorn University  Bangkok Thailand"},{"name":"Center of Excellence in Vaccine Research and Development (Chula Vaccine Research Center, Chula VRC), Faculty of Medicine, Chulalongkorn University  Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7565-9211","authenticated-orcid":false,"given":"Eakachai","family":"Prompetchara","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Faculty of Medicine Chulalongkorn University  Bangkok Thailand"},{"name":"Center of Excellence in Vaccine Research and Development (Chula Vaccine Research Center, Chula VRC), Faculty of Medicine, Chulalongkorn University  Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,26]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/ijlh.13702"},{"key":"e_1_2_13_3_1","volume-title":"Collection, Transport, and Processing of Blood Specimens for Testing Plasma\u2010Based Coagulation Assays, 6th Ed. 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