{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T10:22:05Z","timestamp":1788430925779,"version":"build-2803163510"},"reference-count":28,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T00:00:00Z","timestamp":1788393600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T00:00:00Z","timestamp":1788393600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>JAK2 V617F variant allele frequency (VAF) reflects clonal burden in myeloproliferative neoplasms, but the clinical associations of low\u2010level VAF in patients with erythrocytosis remain uncertain.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>The objective of this study is to examine associations between diagnostic JAK2 V617F VAF categories and clinical, laboratory, bone marrow and thrombotic outcomes in patients with JAK2 V617F\u2010positive erythrocytosis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Patients and Methods<\/jats:title>\n                    <jats:p>\n                      This single\u2010centre retrospective cohort included 174 adults. Patients were stratified by diagnostic VAF as low (&lt;\u20095%;\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200962), intermediate (5%\u201350%;\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200981) or high (&gt;\u200950%;\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200931). Clinical and laboratory characteristics, bone marrow findings and incident thrombotic events during follow\u2010up were compared. Multivariable logistic regression was used to evaluate factors associated with thrombosis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Follow\u2010up duration was comparable amongst the VAF groups (median, 5.0 vs. 8.0 vs. 6.0\u2009years;\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.227). Thrombosis rates increased across the groups (12.9% vs. 22.2% vs. 48.4%;\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.001), as did splenomegaly (25.8% vs. 53.1% vs. 64.5%;\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001) and myelofibrosis transformation (1.6% vs. 13.6% vs. 20.0%;\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.011). Amongst patients with available measurements, median serum erythropoietin decreased with higher VAF (8.0 vs. 3.1 vs. 2.2\u2009mIU\/mL;\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001), whereas the proportion with subnormal erythropoietin increased (17.8% vs. 47.6% vs. 82.6%;\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001). Amongst biopsied patients, polycythemia vera\u2010supportive bone marrow morphology was present in 50.0%, 93.5% and 100% of the groups respectively. A diagnostic VAF\u2009&gt;\u200950% was independently associated with subsequent thrombosis (OR: 3.35; 95% CI: 1.06\u201310.58;\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.039), as was platelet count (OR: 9.92; 95% CI: 1.52\u201364.63;\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.016).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>A diagnostic JAK2 V617F VAF\u2009&gt;\u200950% was independently associated with subsequent thrombosis in this cohort. The low\u2010VAF group was diagnostically and clinically heterogeneous. These findings should be considered hypothesis\u2010generating and require prospective validation.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/ijlh.70242","type":"journal-article","created":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T10:09:01Z","timestamp":1788430141000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Clinical and Laboratory Associations of\n                    <scp>JAK2 V617F<\/scp>\n                    Variant Allele Frequency in Patients With\n                    <scp>JAK2 V617F<\/scp>\n                    \u2010Positive Erythrocytosis: A Single\u2010Centre Retrospective Analysis"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2164-9776","authenticated-orcid":false,"given":"Tahir Alper","family":"Cinli","sequence":"first","affiliation":[{"name":"Department of Hematology Ba\u015fak\u015fehir \u00c7am ve Sakura City Hospital  Istanbul Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4154-7865","authenticated-orcid":false,"given":"\u00c7isem","family":"\u00c7\u0131nar","sequence":"additional","affiliation":[{"name":"Department of Medical Genetics Van Regional Training and Research Hospital  Van 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