{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T02:08:18Z","timestamp":1788833298947,"version":"build-2803163510"},"reference-count":19,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T00:00:00Z","timestamp":1788739200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T00:00:00Z","timestamp":1788739200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"abstract":"ABSTRACT<\/jats:title>\n \n Background<\/jats:title>\n Pooled normal plasma (PNP) used for lupus anticoagulant (LA) mixing tests may carry platelet\u2010derived phospholipid (PL) activity introduced during freezing\/lyophilization, potentially attenuating LA\u2010mediated prolongation of clotting times.<\/jats:p>\n <\/jats:sec>\n \n Objectives<\/jats:title>\n To determine whether thrombin generation assay (TGA) under low\u2010PL conditions functionally captures endogenous, platelet\u2010derived PL activity in commercial PNP and relates to LA mixing outcomes.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n Four commercial PNPs (CRYOcheck PNP, Coagtrol N, SHP, Ci\u2010Trol 1) were tested by TGA at PL 4, 1, 0.1, and 0\u2009\u03bcM with 1\u2009pM tissue factor and 1.6\u2009\u03bcM corn trypsin inhibitor. LA weak\u2010positive and LA positive controls were mixed 1:1 with each PNP and assessed by five APTT reagents to compute the index of circulating anticoagulant (ICA). Platelet factor 4 (PF4) levels were measured by ELISA.<\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n SHP and Ci\u2010Trol 1 showed consistently higher TGA peak heights than CRYOcheck PNP and Coagtrol N across all PL concentrations; thrombin generation persisted at 0\u2009\u03bcM PL, consistent with activity in the absence of added PL. The other TGA parameters exhibited the same trend. Mean ICA values were inversely correlated with TGA peak height at 4 and 1\u2009\u03bcM PL (\n r<\/jats:italic>\n \u2009\u2264\u2009\u22120.985), with direction preserved at 0.1 and 0\u2009\u03bcM. PF4 levels were markedly higher in SHP and Ci\u2010Trol 1 and paralleled higher TGA peak height and lower ICA.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n Endogenous, platelet\u2010derived PL activity in commercial PNP is a key determinant of the LA mixing test performance. TGA under low\u2010PL conditions, complemented by PF4 measurement, offers a practical approach to qualify PNP lots and reduce the under recognition of LA.<\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/ijlh.70247","type":"journal-article","created":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T01:46:02Z","timestamp":1788831962000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Functional Assessment of Platelet\u2010Derived Phospholipid Activity in Pooled Normal Plasmas by Low\u2010Phospholipid Thrombin Generation Assay and Its Impact on Lupus Anticoagulant Mixing Tests"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6650-0567","authenticated-orcid":false,"given":"Osamu","family":"Kumano","sequence":"first","affiliation":[{"name":"Health and Medical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST) Takamatsu 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