{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T15:42:55Z","timestamp":1789486975603,"version":"build-2803163510"},"reference-count":10,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T00:00:00Z","timestamp":1789430400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T00:00:00Z","timestamp":1789430400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Lab Hematology"],"abstract":"ABSTRACT<\/jats:title>\n \n Introduction<\/jats:title>\n Red blood cell (RBC) biomechanics are key determinants of blood rheology, microcirculatory flow, and tissue oxygen delivery, yet remain underutilized in clinical practice because of methodological variability and limited standardization. This study aimed to define preliminary reference distributions for RBC rheological parameters using the automated MIZAR syllectometry platform and to characterize inter\u2010parameter relationships using exploratory multivariate analyses.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n Whole blood samples from 70 healthy adults were analyzed to generate 13 MIZAR parameters reflecting RBC deformability, aggregation, and elasticity. Conventional statistical methods were used to define reference distributions and assess demographic influences. Principal component analysis (PCA), hierarchical clustering, and correlation analysis were applied to explore covariance structure and statistical interrelationships within the dataset.<\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n Within\u2010run precision was high for several MIZAR parameters, particularly at 800\u2009nm, with mean coefficients of variation <\u20092% for AI, Int(a), AMP, T1\/2, OTF, OT, and ED. Most parameters demonstrated minimal age\u2010related variation. Several parameters differed by sex, particularly those related to aggregation amplitude and optical measurements. PCA showed that the first two components explained 60.9% of total variance, while hierarchical clustering identified five exploratory parameter groupings broadly consistent with their measured rheological characteristics. Correlation analysis further characterized inter\u2010parameter associations.<\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n These findings provide the first preliminary reference dataset for MIZAR\u2010derived RBC parameters and identify patterns of covariance and interrelationships within a healthy cohort. Integrating conventional and exploratory multivariate analyses provides a framework for characterizing interrelated hemorheological measurements. Validation in independent and clinically diverse populations is required to determine the reproducibility and clinical utility of these findings.<\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/ijlh.70251","type":"journal-article","created":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T13:36:40Z","timestamp":1789479400000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Characterizing Red Blood Cell Rheological Parameters Using Automated Syllectometry: Conventional and Exploratory Multivariate Analyses"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-0710-8348","authenticated-orcid":false,"given":"Baran","family":"Dastaran","sequence":"first","affiliation":[{"name":"Department of Biomedical and Molecular Sciences, School of Medicine Queen's University Kingston Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yousra","family":"Tera","sequence":"additional","affiliation":[{"name":"Department of Biomedical and Molecular Sciences, School of Medicine Queen's University Kingston Ontario Canada"},{"name":"Clinical Pathology Department, Faculty of Medicine Mansoura University Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maica Jhiezl","family":"Yunon","sequence":"additional","affiliation":[{"name":"School of Baccalaureate Nursing, St. Lawrence College Kingston Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7562-203X","authenticated-orcid":false,"given":"Maha","family":"Othman","sequence":"additional","affiliation":[{"name":"Department of Biomedical and Molecular Sciences, School of Medicine Queen's University Kingston Ontario Canada"},{"name":"Clinical Pathology Department, Faculty of Medicine Mansoura University Egypt"},{"name":"School of Baccalaureate Nursing, St. Lawrence College Kingston Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,15]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"crossref","first-page":"20808","DOI":"10.1073\/pnas.1210236109","article-title":"Full Dynamics of a Red Blood Cell in Shear Flow","volume":"109","author":"Dupire J.","year":"2012","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"key":"e_1_2_9_3_1","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1055\/s-2003-44551","article-title":"Blood Rheology and Hemodynamics","volume":"29","author":"Baskurt O. K.","year":"2003","journal-title":"Seminars in Thrombosis and Hemostasis"},{"key":"e_1_2_9_4_1","doi-asserted-by":"crossref","first-page":"4065","DOI":"10.1016\/j.jbiomech.2015.10.002","article-title":"Microfluidic Deformability Analysis of the Red Cell Storage Lesion","volume":"48","author":"Matthews K.","year":"2015","journal-title":"Journal of Biomechanics"},{"issue":"1","key":"e_1_2_9_5_1","first-page":"19","article-title":"The Impact of Measurement Techniques and Common Effectors on Red Blood Cell Aggregation","volume":"60","author":"Chien K.","year":"2025","journal-title":"Biorheology"},{"key":"e_1_2_9_6_1","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1109\/TBME.2002.807319","article-title":"Syllectometry: The Effect of Aggregometer Geometry in the Assessment of Red Blood Cell Shape Recovery and Aggregation","volume":"50","author":"Dobbe J. G. G.","year":"2003","journal-title":"IEEE Transactions on Biomedical Engineering"},{"key":"e_1_2_9_7_1","unstructured":"ALCOR Scientific \u201cMIZAR. ALCOR Scientific \u201daccessed March 15 2026 https:\/\/alcorscientific.com\/mizar\/."},{"key":"e_1_2_9_8_1","doi-asserted-by":"crossref","first-page":"263","DOI":"10.3233\/CH-2010-1303","article-title":"Gender Differences of Blood Rheological Parameters in Laboratory Animals","volume":"45","author":"Nemeth N.","year":"2010","journal-title":"Clinical Hemorheology and Microcirculation"},{"key":"e_1_2_9_9_1","doi-asserted-by":"crossref","first-page":"1547","DOI":"10.1177\/0271678X211065204","article-title":"Cerebrovascular Responses to Somatomotor Stimulation in Parkinson's Disease: A Multivariate Analysis","volume":"42","author":"Barnes S. C.","year":"2022","journal-title":"Journal of Cerebral Blood Flow and Metabolism"},{"key":"e_1_2_9_10_1","doi-asserted-by":"crossref","first-page":"454","DOI":"10.3389\/fimmu.2020.00454","article-title":"The Red Blood Cell\u2014Inflammation Vicious Circle in Sickle Cell Disease","volume":"11","author":"Nader E.","year":"2020","journal-title":"Frontiers in Immunology"},{"key":"e_1_2_9_11_1","doi-asserted-by":"crossref","first-page":"635","DOI":"10.3233\/CH-168037","article-title":"Interrelation of Blood Coagulation and Hemorheology in Cancer","volume":"64","author":"Tikhomirova I.","year":"2017","journal-title":"Ch"}],"container-title":["International Journal of Laboratory Hematology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ijlh.70251","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/ijlh.70251","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ijlh.70251","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T13:36:43Z","timestamp":1789479403000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ijlh.70251"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,15]]},"references-count":10,"alternative-id":["10.1111\/ijlh.70251"],"URL":"https:\/\/doi.org\/10.1111\/ijlh.70251","archive":["Portico"],"relation":{},"ISSN":["1751-5521","1751-553X"],"issn-type":[{"value":"1751-5521","type":"print"},{"value":"1751-553X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,15]]},"assertion":[{"value":"2026-05-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-02","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-15","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"ijlh.70251"}}