{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T10:14:52Z","timestamp":1784542492040,"version":"3.55.0"},"reference-count":29,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2016,4,5]],"date-time":"2016-04-05T00:00:00Z","timestamp":1459814400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Italian Ministry of Health","award":["GR-2010 2304957"],"award-info":[{"award-number":["GR-2010 2304957"]}]},{"name":"European Community","award":["FP7-ICT-2012-600932"],"award-info":[{"award-number":["FP7-ICT-2012-600932"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pediatric Obesity"],"published-print":{"date-parts":[[2017,6]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Circulating microRNAs (miRNAs) may act as biomarkers of metabolic disturbances.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>The aim of this study was to identify serum miRNAs signature of early insulin resistance in obese preschoolers.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Twelve obese children, aged 2\u20136\u2009years, six insulin resistant (IR) and six controls were selected being age\u2010matched, sex\u2010matched and body mass index\u2010matched. Profiling of 179 circulating miRNAs, known to be widely expressed in the bloodstream, was investigated by quantitative polymerase chain reaction at fasting and 120\u2009min following a standard oral glucose tolerance test (OGTT).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Twenty\u2010one miRNAs were differentially regulated in IR obese preschoolers. miR\u2010200c\u20103p, miR\u2010190a and miR\u201095 were differently regulated both at fasting and 120\u2009min after the OGTT. In controls, the fold changes of some miRNAs were correlated with \u0394glucose<jats:sub>0\u2010120<\/jats:sub> (miR\u2010660, miR\u201026b\u20105p and miR\u201022\u20103p: <jats:italic>p<\/jats:italic>\u2009=\u20090.005 for all) and \u0394insulin<jats:sub>0\u2010120<\/jats:sub> (miR\u2010660 and miR\u201022\u20103p: <jats:italic>p<\/jats:italic>\u2009=\u20090.02 for both and miR\u2010423\u20105p: <jats:italic>p<\/jats:italic>\u2009=\u20090.042). In IR patients, miR\u20101 fold changes were correlated with \u0394glucose<jats:sub>0\u2010120<\/jats:sub> <jats:sub>(<\/jats:sub><jats:italic>r\u2009=<\/jats:italic>\u2009\u22120.786; <jats:italic>p<\/jats:italic>\u2009=\u20090.036) and \u0394insulin<jats:sub>0\u2010120<\/jats:sub> <jats:sub>(<\/jats:sub><jats:italic>r\u2009=<\/jats:italic>\u2009\u22120.821; <jats:italic>p<\/jats:italic>\u2009=\u20090.023).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our study identifies circulating miR\u2010200c\u20103p, miR\u2010190a and miR\u201095 as biomarkers of insulin resistance in obese preschoolers, being differentially regulated in IR patients both in fasting condition and after the OGTT. Expression of some circulating miRNAs seems reflecting glucose and insulin excursion following the OGTT differently in controls and IR obese preschoolers.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijpo.12133","type":"journal-article","created":{"date-parts":[[2016,4,10]],"date-time":"2016-04-10T12:22:46Z","timestamp":1460290966000},"page":"229-238","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":36,"title":["Oral glucose tolerance test unravels circulating miRNAs associated with insulin resistance in obese preschoolers"],"prefix":"10.1111","volume":"12","author":[{"given":"A.","family":"Masotti","sequence":"first","affiliation":[{"name":"Gene expression \u2013 Microarrays Laboratory Bambino Ges\u00f9 Children's Hospital, IRCCS  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Baldassarre","sequence":"additional","affiliation":[{"name":"Gene expression \u2013 Microarrays Laboratory Bambino Ges\u00f9 Children's Hospital, IRCCS  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Fabrizi","sequence":"additional","affiliation":[{"name":"Research Unit for Multifactorial Disease, Scientific Directorate Bambino Ges\u00f9 Children's Hospital, IRCCS  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Olivero","sequence":"additional","affiliation":[{"name":"Federazione Italiana Medici Pediatri, FIMP  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. 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