{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T19:26:06Z","timestamp":1784921166615,"version":"3.55.0"},"reference-count":35,"publisher":"Wiley","issue":"S1","license":[{"start":{"date-parts":[[2016,12,8]],"date-time":"2016-12-08T00:00:00Z","timestamp":1481155200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"National Institutes of Child Health and Human Development","award":["K12 HD055884"],"award-info":[{"award-number":["K12 HD055884"]}]},{"name":"Genentech Growth Disorders","award":["G-29954"],"award-info":[{"award-number":["G-29954"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pediatric Obesity"],"published-print":{"date-parts":[[2017,8]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Neonatal adiposity has many determinants and may be a risk factor for future obesity. Epigenetic regulation of metabolically important genes is a potential contributor.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>The objective of the study is to determine whether methylation changes in the<jats:italic>LEP<\/jats:italic>gene in cord blood DNA are impacted by the maternal environment or affect neonatal adiposity measures.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A cross\u2010sectional study of 114 full\u2010term neonates born to healthy mothers with normal glucose tolerance was performed. Cord blood was assayed for leptin and genome\u2010wide DNA methylation profiles via the Illumina 450K platform. Neonatal body composition was measured by air displacement plethysmography. Multivariate linear regression models and semi\u2010partial correlation coefficients were used to analyze associations. False discovery rate was estimated to account for multiple comparisons.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Maternal pre\u2010pregnancy BMI was associated with decreased methylation at five CpG sites near the<jats:italic>LEP<\/jats:italic>transcription start site in an adjusted model (false discovery rate &lt;0.022 for each site). The association between maternal BMI and cord blood leptin approached significance (<jats:italic>r<\/jats:italic>\u2009=\u20090.18,<jats:italic>p<\/jats:italic>\u2009=\u20090.054). Cord blood leptin was positively correlated with neonatal adiposity measures including birth weight (<jats:italic>r<\/jats:italic>\u2009=\u20090.45,<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001), fat mass (<jats:italic>r<\/jats:italic>\u2009=\u20090.47,<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001) and percent body fat (<jats:italic>r<\/jats:italic>\u2009=\u20090.44,<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Maternal pre\u2010pregnancy BMI is strongly associated with decreased cord blood<jats:italic>LEP<\/jats:italic>gene methylation and may mediate the well\u2010known association between maternal pre\u2010pregnancy BMI and neonatal adiposity.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ijpo.12204","type":"journal-article","created":{"date-parts":[[2016,12,9]],"date-time":"2016-12-09T01:22:40Z","timestamp":1481246560000},"page":"57-64","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":37,"title":["Maternal pre\u2010pregnancy BMI downregulates neonatal cord blood<i>LEP<\/i>methylation"],"prefix":"10.1111","volume":"12","author":[{"given":"R.","family":"Kadakia","sequence":"first","affiliation":[{"name":"Division of Endocrinology Ann and Robert H. Lurie Children's Hospital of Chicago Chicago IL USA"},{"name":"Department of Pediatrics Northwestern University Feinberg School of Medicine Chicago IL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Preventive Medicine Northwestern University Feinberg School of Medicine Chicago IL USA"},{"name":"Health Sciences Integrated PhD Program Northwestern University Feinberg School of Medicine Chicago IL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Z.","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Preventive Medicine Northwestern University Feinberg School of Medicine Chicago IL USA"},{"name":"Driskill Graduate Program in Life Sciences Northwestern University Feinberg School of Medicine Chicago IL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W.","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Preventive Medicine Northwestern University Feinberg School of Medicine Chicago IL USA"},{"name":"Robert H. 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