{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T16:14:19Z","timestamp":1791216859141,"version":"4.1.0"},"reference-count":32,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2013,2,4]],"date-time":"2013-02-04T00:00:00Z","timestamp":1359936000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int J of Urology"],"published-print":{"date-parts":[[2013,9]]},"abstract":"<jats:sec><jats:title>Objectives<\/jats:title><jats:p>To investigate the potential mechanism of development of resistance to tyrosine kinase inhibitor in renal cell carcinoma.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A primary culture of renal cell carcinoma cells (<jats:styled-content style=\"fixed-case\">KMRM<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">S<\/jats:styled-content>2) was established from an advanced renal cell carcinoma patient with cutaneous metastasis, who had not responded to sorafenib. A total of 84 human angiogenesis\u2010related genes were compared between cutaneous metastasis and the primary tumor by real time polymerase chain reaction. Spectral karyotyping and cell proliferation assay were carried out to determine the biological features of the cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Primary tumor was histopathologically diagnosed as high\u2010grade clear cell carcinoma with sarcomatoid change and rhabdoid features. The cutaneous metastasis also consisted of sarcomatoid components. Expression levels of many angiogenesis\u2010related genes in the cutaneous metastasis were relatively higher than those of primary tumor. Chromosomal analysis of the <jats:styled-content style=\"fixed-case\">KMRM<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">S<\/jats:styled-content>2 showed cytogenetic abnormalities with hypertriploidy and translocation. <jats:italic>In\u2009vitro<\/jats:italic> proliferation assay showed the relatively higher resistance of <jats:styled-content style=\"fixed-case\">KMRM<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">S<\/jats:styled-content>2 against sorafenib.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The sarcomatoid change and rhabdoid features of renal cell carcinoma with cytogenetic hyperploidy might be associated with elevated expression of angiogenesis\u2010related genes, which leads to resistance against tyrosine kinase inhibitor. The present study might contribute to discovering novel therapeutic targets for the treatment of patients with advanced renal cell carcinoma resistant to tyrosine kinase inhibitor.<\/jats:p><\/jats:sec>","DOI":"10.1111\/iju.12084","type":"journal-article","created":{"date-parts":[[2013,2,5]],"date-time":"2013-02-05T03:57:21Z","timestamp":1360036641000},"page":"923-930","source":"Crossref","is-referenced-by-count":9,"title":["Expression of angiogenesis\u2010related gene profiles and development of resistance to tyrosine\u2010kinase inhibitor in advanced renal cell carcinoma: Characterization of sorafenib\u2010resistant cells derived from a cutaneous metastasis"],"prefix":"10.1111","volume":"20","author":[{"given":"Takashi","family":"Karashima","sequence":"first","affiliation":[{"name":"Department of Urology Kochi University  Nankoku City Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hideo","family":"Fukuhara","sequence":"additional","affiliation":[{"name":"Department of Urology Kochi University  Nankoku City Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenji","family":"Tamura","sequence":"additional","affiliation":[{"name":"Department of Urology Kochi University  Nankoku City Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shingo","family":"Ashida","sequence":"additional","affiliation":[{"name":"Department of Urology Kochi University  Nankoku City Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masayuki","family":"Kamada","sequence":"additional","affiliation":[{"name":"Department of Urology Kochi University  Nankoku City Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keiji","family":"Inoue","sequence":"additional","affiliation":[{"name":"Department of Urology Kochi University  Nankoku City Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takahiro","family":"Taguchi","sequence":"additional","affiliation":[{"name":"Department of Human Health and Medical Science Faculty of Medicine Kochi University  Nankoku City Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naoto","family":"Kuroda","sequence":"additional","affiliation":[{"name":"Department of Pathology Kochi Red Cross Hospital  Kochi City Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taro","family":"Shuin","sequence":"additional","affiliation":[{"name":"Department of Urology Kochi University  Nankoku City Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,2,4]]},"reference":[{"key":"e_1_2_8_2_1","first-page":"160","article-title":"Renal cell carcinoma: presentation, staging, and surgical treatment","volume":"27","author":"Russo P","year":"2000","journal-title":"Semin. Oncol."},{"key":"e_1_2_8_3_1","first-page":"1541","article-title":"Chemotherapy for renal cell carcinoma","volume":"19","author":"Hartmann JT","year":"1999","journal-title":"Anticancer Res."},{"key":"e_1_2_8_4_1","first-page":"141","article-title":"Vaccine and gene therapy of renal cell carcinoma","volume":"19","author":"Gitlitz BJ","year":"2001","journal-title":"Semin. Urol. Oncol."},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(197205)29:5<1298::AID-CNCR2820290526>3.0.CO;2-6"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa060655"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa065044"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1677\/erc.0.0080219"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-040028"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2002.11.126"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02617972"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.3780461"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1349-7006.2005.00018.x"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.273.5274.494"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004180050169"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/1361-6374(199606)4:2<65::AID-BIO4>3.0.CO;2-D"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(83)90303-4"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-04-1443"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-040028"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2010.06.032"},{"key":"e_1_2_8_21_1","unstructured":"National Comprehensive Cancer Network.NCCN clinical practice guideline in oncologyTM\u2010ver.2.2010. Kidney Cancer 2010."},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(05)65384-0"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.20541"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.1999.17.2.523"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2008.19.5511"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1093\/annonc\/mdq651"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.4076\/1757-1626-2-7948"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9896(199710)183:2<131::AID-PATH931>3.0.CO;2-G"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2007.15.8345"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/20459"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.284.5413.455"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/35017054"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464-410X.2006.06376.x"}],"container-title":["International Journal of Urology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fiju.12084","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/iju.12084","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,4]],"date-time":"2023-10-04T00:09:09Z","timestamp":1696378149000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/iju.12084"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,2,4]]},"references-count":32,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2013,9]]}},"alternative-id":["10.1111\/iju.12084"],"URL":"https:\/\/doi.org\/10.1111\/iju.12084","archive":["Portico"],"relation":{},"ISSN":["0919-8172","1442-2042"],"issn-type":[{"value":"0919-8172","type":"print"},{"value":"1442-2042","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,2,4]]}}}