{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,12]],"date-time":"2025-12-12T13:24:20Z","timestamp":1765545860356},"reference-count":39,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2013,8,29]],"date-time":"2013-08-29T00:00:00Z","timestamp":1377734400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int J of Urology"],"published-print":{"date-parts":[[2014,3]]},"abstract":"<jats:sec><jats:title>Objectives<\/jats:title><jats:p>To study the relationship between myosin light chain phosphorylation of the detrusor muscle and spontaneous smooth muscle contractions in a rabbit model of partial outlet obstruction.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">N<\/jats:styled-content>ew <jats:styled-content style=\"fixed-case\">Z<\/jats:styled-content>ealand white rabbit urinary bladders were partially obstructed for 2 weeks. Rabbits were euthanized, detrusor muscle strips were hung on a force transducer and spontaneous activity was measured at varying concentrations (0\u20130.03 \u03bcM\/L) of the <jats:styled-content style=\"fixed-case\">R<\/jats:styled-content>ho\u2010kinase inhibitors <jats:styled-content style=\"fixed-case\">GSK<\/jats:styled-content> 576371 or 0.01 \u03bcM\/L <jats:styled-content style=\"fixed-case\">Y<\/jats:styled-content>27632. Basal myosin light chain phosphorylation was measured by 2\u2010<jats:styled-content style=\"fixed-case\">D<\/jats:styled-content> gel electrophoresis in control and <jats:styled-content style=\"fixed-case\">GSK<\/jats:styled-content> 576371\u2010treated strips.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Both drugs suppressed the force of spontaneous contractions, whereas <jats:styled-content style=\"fixed-case\">GSK<\/jats:styled-content> 576371 had a more profound effect on the frequency of the contractions. The <jats:styled-content style=\"fixed-case\">IC<jats:sub>50<\/jats:sub><\/jats:styled-content> values for the inhibition of frequency and force of spontaneous contractions were 0.17 \u03bcM\/L and 0.023 \u03bcM\/L for <jats:styled-content style=\"fixed-case\">GSK<\/jats:styled-content> 576371, respectively. The compound significantly decreased the basal myosin light chain phosphorylation from 28.0\u2009\u00b1\u20093.9% to 13.5\u2009\u00b1\u20091.9% (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05). At 0.01 \u03bcM\/L, <jats:styled-content style=\"fixed-case\">GSK<\/jats:styled-content> 576371 inhibited spontaneous bladder overactivity by 50%, but inhibited carbachol\u2010elicited contractions force by just 25%.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>These data suggest that Rho\u2010kinase regulation of myosin light chain phosphorylation contributes to the spontaneous detrusor activity induced by obstruction. This finding could have therapeutic implications by providing another therapeutic option for myogenic, overactive bladder.<\/jats:p><\/jats:sec>","DOI":"10.1111\/iju.12247","type":"journal-article","created":{"date-parts":[[2013,8,30]],"date-time":"2013-08-30T03:58:03Z","timestamp":1377835083000},"page":"319-324","source":"Crossref","is-referenced-by-count":6,"title":["Effects of Rho\u2010kinase inhibition on myosin light chain phosphorylation and obstruction\u2010induced detrusor overactivity"],"prefix":"10.1111","volume":"21","author":[{"given":"James O","family":"Marx","sequence":"first","affiliation":[{"name":"School of Veterinary Medicine University of Pennsylvania  Philadelphia USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maureen E","family":"Basha","sequence":"additional","affiliation":[{"name":"Division of Urology University of Pennsylvania  Philadelphia USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sunish","family":"Mohanan","sequence":"additional","affiliation":[{"name":"Division of Urology University of Pennsylvania  Philadelphia USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joseph A","family":"Hypolite","sequence":"additional","affiliation":[{"name":"Division of Urology University of Pennsylvania  Philadelphia USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shaohua","family":"Chang","sequence":"additional","affiliation":[{"name":"Division of Urology University of Pennsylvania  Philadelphia USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alan J","family":"Wein","sequence":"additional","affiliation":[{"name":"Division of Urology University of Pennsylvania  Philadelphia USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen A","family":"Zderic","sequence":"additional","affiliation":[{"name":"The Children's Hospital of Philadelphia  Philadelphia USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicholas J","family":"Laping","sequence":"additional","affiliation":[{"name":"Endo Pharmaceuticals  Chadds Ford Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samuel","family":"Chacko","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine University of Pennsylvania  Philadelphia USA"},{"name":"Division of Urology University of Pennsylvania  Philadelphia USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,8,29]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464-410X.2007.07205.x"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(05)00313-7"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(05)63948-1"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/nau.1002"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00235.2005"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.200409212"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00027.2004"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00274.2002"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00378.2002"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.urology.2007.11.010"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/nau.21193"},{"key":"e_1_2_8_13_1","first-page":"920","article-title":"Calcium sensitization as a pharmacological target in vascular smooth\u2010muscle regulation","volume":"6","author":"Christ G","year":"2005","journal-title":"Curr. 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