{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T19:17:33Z","timestamp":1773947853417,"version":"3.50.1"},"reference-count":30,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2014,3,3]],"date-time":"2014-03-03T00:00:00Z","timestamp":1393804800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int J of Urology"],"published-print":{"date-parts":[[2014,7]]},"abstract":"<jats:sec><jats:title>Objectives<\/jats:title><jats:p>To investigate the contribution of bone marrow\u2010derived mesenchymal stem cells to the changes in bladder morphology in response to partial bladder outlet obstruction.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Allogenic bone marrow cells were transplanted from transgenic rats expressing green fluorescent protein into female <jats:styled-content style=\"fixed-case\">S<\/jats:styled-content>prague\u2013<jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>awley rats 1 day after their bone marrow\u2010derived mesenchymal stem cells had been destroyed by irradiation. This generated chimeric rats in which green fluorescent protein labeled bone marrow\u2010derived mesenchymal stem cells replaced host bone marrow\u2010derived mesenchymal stem cells. The animals received partial bladder outlet obstruction or sham surgery 6 weeks later. The animals were killed 6 weeks after the surgery, and bladder tissue was prepared for immunofluorescence with antibodies against a urothelium marker (<jats:styled-content style=\"fixed-case\">AE<\/jats:styled-content>1\/<jats:styled-content style=\"fixed-case\">AE<\/jats:styled-content>3), a myofibroblast marker (vimentin), a smooth muscle marker and green fluorescent protein.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>More labeled bone marrow\u2010derived mesenchymal stem cells were found in the partial bladder outlet obstruction group than in the in the sham group. Most bone marrow\u2010derived mesenchymal stem cells were present around the basement membrane (laminin) and lamina propria below the urothelium. Bone marrow\u2010derived mesenchymal stem cells were also found in the urothelium layer, and some of them were double\u2010stained with green fluorescent protein and <jats:styled-content style=\"fixed-case\">AE<\/jats:styled-content>1\/<jats:styled-content style=\"fixed-case\">AE<\/jats:styled-content>3. Some bone marrow\u2010derived mesenchymal stem cells, which were located in the interstitial tissue, were double\u2010stained with green fluorescent protein and vimentin. Bone marrow\u2010derived mesenchymal stem cells, which migrated into the smooth muscle layer, showed fusiform morphology, and some were double\u2010stained with green fluorescent protein and smooth muscle marker.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Bone marrow\u2010derived mesenchymal stem cells home to the partial bladder outlet obstruction bladder, and these cells have the potential to differentiate into the several components of bladder tissue including the urothelium, myofibroblasts and smooth muscle cells. Thus, bone marrow\u2010derived mesenchymal stem cells contribute to the morphological changes of the bladder in response to partial bladder outlet obstruction.<\/jats:p><\/jats:sec>","DOI":"10.1111\/iju.12406","type":"journal-article","created":{"date-parts":[[2014,3,4]],"date-time":"2014-03-04T03:47:45Z","timestamp":1393904865000},"page":"714-718","source":"Crossref","is-referenced-by-count":10,"title":["Contribution of bone marrow\u2010derived mesenchymal stem cells to the morphological changes in the bladder after partial outlet obstruction: A preliminary study"],"prefix":"10.1111","volume":"21","author":[{"given":"Yukiko","family":"Kanno","sequence":"first","affiliation":[{"name":"Department of Urology Hokkaido University Graduate School of Medicine  Sapporo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takahiko","family":"Mitsui","sequence":"additional","affiliation":[{"name":"Department of Urology Hokkaido University Graduate School of Medicine  Sapporo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroshi","family":"Sano","sequence":"additional","affiliation":[{"name":"Department of Urology Hokkaido University Graduate School of Medicine  Sapporo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takeya","family":"Kitta","sequence":"additional","affiliation":[{"name":"Department of Urology Hokkaido University Graduate School of Medicine  Sapporo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kimihiko","family":"Moriya","sequence":"additional","affiliation":[{"name":"Department of Urology Hokkaido University Graduate School of Medicine  Sapporo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katsuya","family":"Nonomura","sequence":"additional","affiliation":[{"name":"Department of Urology Hokkaido University Graduate School of Medicine  Sapporo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,3,3]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(17)44485-5"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1442-2042.2012.03217.x"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464-410X.2010.09445.x"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1464-410X.1998.00873.x"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1053\/jhep.2000.9124"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm755"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0404398101"},{"key":"e_1_2_8_9_1","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1111\/j.1757-5672.2012.00153.x","article-title":"Stromal cell\u2010derived factor 1\u03b1 induces accumulation of intraveneously administered marrow\u2010derived stromal cells in the partially obstructed rat bladder","volume":"4","author":"Sano H","year":"2012","journal-title":"LUTS"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2009.02.081"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(97)00313-X"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1159\/000085719"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)62278-X"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-196803000-00009"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2012.11.120"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.2741\/3853"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1253\/circj.69.850"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2005.01.069"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(02)09670-8"},{"key":"e_1_2_8_20_1","first-page":"7","article-title":"Advanced therapies using autologous bone marrow cells for chronic liver disease","volume":"14","author":"Takami T","year":"2012","journal-title":"Discov. 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