{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,28]],"date-time":"2025-05-28T21:10:02Z","timestamp":1748466602760,"version":"3.41.0"},"reference-count":30,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2015,6,24]],"date-time":"2015-06-24T00:00:00Z","timestamp":1435104000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int J of Urology"],"published-print":{"date-parts":[[2015,9]]},"abstract":"<jats:sec><jats:title>Objectives<\/jats:title><jats:p>To evaluate the influence of hypotonic solutions on ureteral relaxation mediated by the release of calcitonin gene\u2010related peptide from intramural sensory nerve endings.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Urine osmolarity of Sprague\u2013Dawley rats drinking water low in salt content (Fiuggi water) or a reference water for 7\u00a0days was measured. Release of calcitonin gene\u2010related peptide\u2010like immunoreactivity from slices of rat ureter and urinary bladder by hypotonic solutions was assessed by an immunometric assay. The mechanism through which hypotonic solutions inhibit neurokinin\u00a0A\u2010induced phasic contractions of isolated rat ureters was evaluated by organ bath studies.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A 7\u2010day consumption of Fiuggi water in rats reduced urine osmolarity by ~40%. Exposure to hypotonic solutions released calcitonin gene\u2010related peptide\u2010like immunoreactivity from slices of rat ureter. This response was abated in a calcium\u2010free medium, after capsaicin desensitization, and in the presence of the unselective transient receptor potential channel antagonist, ruthenium red. Exposure of isolated rat ureteral preparations to a hypotonic solution inhibited neurokinin\u00a0A\u2010evoked phasic contraction. This response was attenuated by capsaicin desensitization and in the presence of the calcitonin gene\u2010related peptide receptor antagonist, calcitonin gene\u2010related peptide<jats:sub>8\u201037<\/jats:sub>. Transient receptor potential vanilloid\u00a01 or transient receptor potential vanilloid 4 antagonists did not affect the neurogenic and calcitonin gene\u2010related peptide\u2010dependent relaxation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Present data show that hypotonic solution evokes calcitonin gene\u2010related peptide release from capsaicin\u2010sensitive intramural sensory nerves, thus inhibiting ureteral contractility, through a transient receptor potential\u2010dependent mechanism. However, this mechanism does not involve transient receptor potential vanilloid\u00a01 or transient receptor potential vanilloid\u00a0 4. Future studies with appropriate<jats:italic>in\u00a0vivo<\/jats:italic>models should investigate the hypothesis that hypostenuric urine diffusing into the ureteral tissue might favor ureteral relaxation through this novel mechanism.<\/jats:p><\/jats:sec>","DOI":"10.1111\/iju.12837","type":"journal-article","created":{"date-parts":[[2015,6,24]],"date-time":"2015-06-24T06:25:00Z","timestamp":1435127100000},"page":"878-883","source":"Crossref","is-referenced-by-count":2,"title":["Ureteral relaxation through calcitonin gene\u2010related peptide release from sensory nerve terminals by hypotonic solution"],"prefix":"10.1111","volume":"22","author":[{"given":"Serena","family":"Materazzi","sequence":"first","affiliation":[{"name":"Section\u00a0of Clinical Pharmacology and Oncology Department of Health Sciences University of Florence Florence Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daiana","family":"Minocci","sequence":"additional","affiliation":[{"name":"Section\u00a0of Clinical Pharmacology and Oncology Department of Health Sciences University of Florence Florence Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gaetano","family":"De Siena","sequence":"additional","affiliation":[{"name":"Section\u00a0of Clinical Pharmacology and Oncology Department of Health Sciences University of Florence Florence Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvia","family":"Benemei","sequence":"additional","affiliation":[{"name":"Section\u00a0of Clinical Pharmacology and Oncology Department of Health Sciences University of Florence Florence Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Romina","family":"Nassini","sequence":"additional","affiliation":[{"name":"Section\u00a0of Clinical Pharmacology and Oncology Department of Health Sciences University of Florence Florence Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,6,24]]},"reference":[{"issue":"3","key":"e_1_2_8_2_1","first-page":"S45","article-title":"Epidemiology of nephrolithiasis","volume":"13","author":"Ramello A","year":"2000","journal-title":"J. Nephrol."},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2012.02.2569"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/MOU.0b013e32802b70bc"},{"issue":"11","key":"e_1_2_8_5_1","first-page":"E1060\u20107","article-title":"Primary prevention of nephrolithiasis is cost\u2010effective for a national healthcare system","volume":"110","author":"Lotan Y","year":"2012","journal-title":"BJU Int."},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(86)90068-3"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-2999(96)00765-0"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0702147"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(17)37090-8"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464-410X.2006.06346.x"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1139\/y95-137"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0810801105"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/39807"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)80564-4"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(91)90218-D"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00365.2003"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.2009.00590.x"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(87)90087-X"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2006.121111"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(91)90433-O"},{"key":"e_1_2_8_21_1","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1016\/S0031-6997(25)06664-5","article-title":"Capsaicin: cellular targets, mechanisms of action, and selectivity for thin sensory neurons","volume":"43","author":"Holzer P","year":"1991","journal-title":"Pharmacol. Rev."},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.ju.0000141498.11720.20"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2014.05.006"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(01)66321-3"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3940(88)90060-2"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1152\/physiolgenomics.2001.4.3.165"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00424-003-1028-9"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/35036318"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn1614"},{"key":"e_1_2_8_30_1","doi-asserted-by":"crossref","first-page":"683","DOI":"10.1016\/S0031-6997(24)01384-X","article-title":"Myogenic and neurogenic factors in the control of pyeloureteral motility and ureteral peristalsis","volume":"50","author":"Santicioli P","year":"1998","journal-title":"Pharmacol. Rev."},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01367661"}],"container-title":["International Journal of Urology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fiju.12837","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/iju.12837","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,28]],"date-time":"2025-05-28T20:53:36Z","timestamp":1748465616000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/iju.12837"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,6,24]]},"references-count":30,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2015,9]]}},"alternative-id":["10.1111\/iju.12837"],"URL":"https:\/\/doi.org\/10.1111\/iju.12837","archive":["Portico"],"relation":{},"ISSN":["0919-8172","1442-2042"],"issn-type":[{"type":"print","value":"0919-8172"},{"type":"electronic","value":"1442-2042"}],"subject":[],"published":{"date-parts":[[2015,6,24]]}}}