{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T18:35:59Z","timestamp":1790706959441,"version":"4.1.0"},"reference-count":33,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2017,2,1]],"date-time":"2017-02-01T00:00:00Z","timestamp":1485907200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002301","name":"Eesti Teadusagentuur","doi-asserted-by":"publisher","award":["IUT34\u201019"],"award-info":[{"award-number":["IUT34\u201019"]}],"id":[{"id":"10.13039\/501100002301","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002301","name":"Eesti Teadusagentuur","doi-asserted-by":"publisher","award":["PUT181"],"award-info":[{"award-number":["PUT181"]}],"id":[{"id":"10.13039\/501100002301","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007821","name":"Tartu \u00dclikool","doi-asserted-by":"publisher","award":["SARMBARENG"],"award-info":[{"award-number":["SARMBARENG"]}],"id":[{"id":"10.13039\/501100007821","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006598","name":"Ettev\u00f5tluse Arendamise Sihtasutus","doi-asserted-by":"publisher","award":["EU48695"],"award-info":[{"award-number":["EU48695"]}],"id":[{"id":"10.13039\/501100006598","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int J of Urology"],"published-print":{"date-parts":[[2017,3]]},"abstract":"<jats:sec><jats:title>Objectives<\/jats:title><jats:p>To profile the seminal microbiome applying next generation sequencing.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Semen samples of 67 men were involved in the study (21 men with and 46 men without prostatitis). Seminal microbiomes were profiled applying the method that uses combinatorial sequence tags attached to <jats:styled-content style=\"fixed-case\">polymerase chain reaction<\/jats:styled-content> primers that amplify the <jats:styled-content style=\"fixed-case\">ribosomal ribonucleic acid<\/jats:styled-content> V6 region. Amplified <jats:styled-content style=\"fixed-case\">polymerase chain reaction<\/jats:styled-content> products were sequenced using an Illumina paired\u2010end protocol on HiSeq2000 platform.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The most abundant phylum in semen was <jats:italic>Firmicutes<\/jats:italic>, comprising nearly half of the sequences found (median 41.7%, quartiles 28.5\u201347.2%) followed by <jats:italic>Bacteroidetes<\/jats:italic>,<jats:italic> Proteobacteria<\/jats:italic> and <jats:italic>Actinobacteria<\/jats:italic>. The counts of lactobacilli were higher in healthy men than prostatitis patients (27% [20.2\u201334.6%] <jats:italic>vs<\/jats:italic> 20.2% [4.9\u201325.0%]; <jats:italic>P<\/jats:italic> = 0.05), especially for <jats:italic>Lactobacillus iners<\/jats:italic>. <jats:italic>Proteobacteria<\/jats:italic> comprised higher proportions in prostatitis patients than healthy men. The species richness was higher in prostatitis patients than healthy men (inverted Simpson index 13.5 \u00b1 5.8 <jats:italic>vs<\/jats:italic> 10.3 \u00b1 4.0).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The semen of chronic prostatitis patients contains fewer health\u2010supporting lactobacilli, and has higher species diversity than that of healthy men. <jats:italic>Firmicutes<\/jats:italic> (especially lactobacilli), <jats:italic>Bacteroidetes, Proteobacteria<\/jats:italic> and <jats:italic>Actinobacteria<\/jats:italic> comprise the highest proportion of seminal microbiome.<\/jats:p><\/jats:sec>","DOI":"10.1111\/iju.13286","type":"journal-article","created":{"date-parts":[[2017,2,2]],"date-time":"2017-02-02T00:48:25Z","timestamp":1485996505000},"page":"211-216","source":"Crossref","is-referenced-by-count":117,"title":["Seminal microbiome in men with and without prostatitis"],"prefix":"10.1111","volume":"24","author":[{"given":"Reet","family":"M\u00e4ndar","sequence":"first","affiliation":[{"name":"Competence Center on Health Technologies Tartu Estonia"},{"name":"Department of Microbiology Faculty of Medicine University of Tartu Tartu Estonia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margus","family":"Punab","sequence":"additional","affiliation":[{"name":"Competence Center on Health Technologies Tartu Estonia"},{"name":"Andrology Center Tartu University Hospital Tartu Estonia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"Korrovits","sequence":"additional","affiliation":[{"name":"Competence Center on Health Technologies Tartu Estonia"},{"name":"Department of Microbiology Faculty of Medicine University of Tartu Tartu Estonia"},{"name":"Andrology Center Tartu University Hospital Tartu Estonia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silver","family":"T\u00fcrk","sequence":"additional","affiliation":[{"name":"Department of Microbiology Faculty of Medicine University of Tartu Tartu Estonia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristo","family":"Ausmees","sequence":"additional","affiliation":[{"name":"Andrology Center Tartu University Hospital Tartu Estonia"},{"name":"MediTA Clinic Tartu Estonia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eleri","family":"Lapp","sequence":"additional","affiliation":[{"name":"Competence Center on Health Technologies Tartu Estonia"},{"name":"Department of Microbiology Faculty of Medicine University of Tartu Tartu Estonia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jens\u2010Konrad","family":"Preem","sequence":"additional","affiliation":[{"name":"Competence Center on Health Technologies Tartu Estonia"},{"name":"Faculty of Science and Technology University of Tartu Tartu Estonia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristjan","family":"Oopkaup","sequence":"additional","affiliation":[{"name":"Competence Center on Health Technologies Tartu Estonia"},{"name":"Faculty of Science and Technology University of Tartu Tartu Estonia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andres","family":"Salumets","sequence":"additional","affiliation":[{"name":"Competence Center on Health Technologies Tartu Estonia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaak","family":"Truu","sequence":"additional","affiliation":[{"name":"Competence Center on Health Technologies Tartu Estonia"},{"name":"Faculty of Science and Technology University of Tartu Tartu Estonia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,2]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(05)66541-X"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/QCO.0000000000000024"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/MOU.0b013e3283656a55"},{"key":"e_1_2_8_5_1","volume-title":"The Prostatitis Manual","author":"Nickel JC","year":"2002"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.urology.2007.12.082"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0094991"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.2164\/jandrol.111.014654"},{"key":"e_1_2_8_9_1","first-page":"31","article-title":"Chronic prostatitis: an infectious disease?","volume":"13","author":"Nickel JC","year":"2000","journal-title":"Infect. 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