{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T11:57:58Z","timestamp":1784030278669,"version":"3.55.0"},"reference-count":29,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,10,7]],"date-time":"2020-10-07T00:00:00Z","timestamp":1602028800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J of Urology"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>To study the role of transient receptor potential melastatin\u00a02 in bladder function and inflammation\u2010associated hypersensitivity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We evaluated physiological function of the bladder and intravesical lipopolysaccharide\u2010induced inflammatory nociceptive responses in female wild\u2010type and transient receptor potential melastatin\u00a02\u2010knockout mice. <jats:italic>In\u00a0vivo<\/jats:italic> frequency\/volume and decerebrated unanesthetized cystometry measurements, as well as <jats:italic>in\u00a0vitro<\/jats:italic> detrusor strip functional studies, were carried out to evaluate bladder function. Mice received intravesical lipopolysaccharide (2.0\u00a0mg\/mL) or saline instillation to evaluate responses to bladder inflammation. Voiding and bladder pain\u2010like behaviors, cystometry measurements and histological evaluation were carried out before and after intravesical lipopolysaccharide instillation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Few phenotypic differences in <jats:italic>in\u00a0vivo<\/jats:italic> and <jats:italic>in\u00a0vitro<\/jats:italic> physiological function were found between the two genotypes. Comparison of measurements taken before and 24\u201348\u00a0h after intravesical lipopolysaccharide instillation showed that voiding parameters did not change in transient receptor potential melastatin\u00a02\u2010knockout mice, whereas an increased voiding frequency was observed in wild\u2010type mice. At 24\u00a0h after intravesical lipopolysaccharide instillation, the numbers of bladder pain\u2010like behaviors and of infiltrated inflammatory cells in the bladder submucosal layer were significantly increased, and the voided volume and the intercontraction interval were significantly decreased on cystometry measurements in wild\u2010type mice compared with those in both transient receptor potential melastatin\u00a02\u2010knockout mice and in wild\u2010type mice treated with saline instillation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Although the physiological roles of transient receptor potential melastatin 2 channels in the bladder might be limited, inflammation and associated hypersensitivity of the bladder caused by intravesical lipopolysaccharide instillation are attenuated in transient receptor potential melastatin\u00a02\u2010knockout mice, suggesting pathophysiological roles of transient receptor potential melastatin\u00a02 channels in these processes.<\/jats:p><\/jats:sec>","DOI":"10.1111\/iju.14389","type":"journal-article","created":{"date-parts":[[2020,10,7]],"date-time":"2020-10-07T15:08:55Z","timestamp":1602083335000},"page":"107-114","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Lacking transient receptor potential melastatin\u00a02 attenuates lipopolysaccharide\u2010induced bladder inflammation and its associated hypersensitivity in mice"],"prefix":"10.1111","volume":"28","author":[{"given":"Jun","family":"Kamei","sequence":"first","affiliation":[{"name":"Department of Urology Jichi Medical University Tochigi Japan"},{"name":"Department of Continence Medicine The University of Tokyo Graduate School of Medicine Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naoki","family":"Aizawa","sequence":"additional","affiliation":[{"name":"Department of Continence Medicine The University of Tokyo Graduate School of 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