{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T14:14:17Z","timestamp":1788185657004,"version":"build-2803163510"},"reference-count":30,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,12,1]],"date-time":"2020-12-01T00:00:00Z","timestamp":1606780800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["JP18bk0104057"],"award-info":[{"award-number":["JP18bk0104057"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J of Urology"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>To assess the characteristics of adipose\u2010derived regenerative cells, and provide supportive data explaining the mechanism of efficacy observed for the use of these cells in the treatment of stress urinary incontinence.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Adipose tissues were harvested by abdominal liposuction from healthy donors and patients with stress urinary incontinence. Adipose\u2010derived regenerative cells were isolated from tissues using the Celution system, and assessed for their characteristics and ability to differentiate into smooth muscle cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Adipose\u2010derived regenerative cells isolated by the Celution system developed into fibroblastic colonies. Flow cytometric analysis of adipose\u2010derived stem cell markers showed that adipose\u2010derived regenerative cells were positive for CD34 and CD44, and negative for CD31. Immunofluorescence staining after differentiation showed that colony\u2010forming cells were positive for alpha\u2010smooth muscle actin, calponin and desmin, which are smooth muscle cell markers. A cytokine release assay showed that adherent cells secreted cytokines associated with angiogenesis, including vascular endothelial growth factor\u2010A, angiopoietin\u20102 and placental growth factor.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Adipose\u2010derived regenerative cells collected by the Celution system might have clonogenic capacity and an angiogenetic function. These properties might contribute to the mechanisms through which regenerative cell therapy by periurethral injection of autologous adipose\u2010derived regenerative cells ameliorates stress urinary incontinence.<\/jats:p><\/jats:sec>","DOI":"10.1111\/iju.14408","type":"journal-article","created":{"date-parts":[[2020,12,2]],"date-time":"2020-12-02T06:58:07Z","timestamp":1606892287000},"page":"115-124","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Biological characterization of adipose\u2010derived regenerative cells used for the treatment of stress urinary incontinence"],"prefix":"10.1111","volume":"28","author":[{"given":"Yuka Tsukagoshi","family":"Okabe","sequence":"first","affiliation":[{"name":"Departments of Department of Advanced Medicine Nagoya University Hospital Nagoya Aichi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shinobu","family":"Shimizu","sequence":"additional","affiliation":[{"name":"Departments of Department of Advanced Medicine Nagoya University Hospital Nagoya Aichi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yukihiro","family":"Suetake","sequence":"additional","affiliation":[{"name":"Departments of Department of Advanced Medicine Nagoya University Hospital Nagoya Aichi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hisako","family":"Matsui\u2010Hirai","sequence":"additional","affiliation":[{"name":"Departments of Department of Advanced Medicine Nagoya University Hospital Nagoya Aichi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shizuka","family":"Hasegawa","sequence":"additional","affiliation":[{"name":"Department of Clinical Engineering Nagoya University Hospital Nagoya Aichi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keisuke","family":"Takanari","sequence":"additional","affiliation":[{"name":"Department of Plastic and Reconstructive Surgery Nagoya University Graduate School of Medicine Nagoya Aichi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuhiro","family":"Toriyama","sequence":"additional","affiliation":[{"name":"Department of Plastic and Reconstructive Surgery Nagoya University Graduate School of Medicine Nagoya Aichi Japan"},{"name":"Department of Plastic and Reconstructive Surgery Nagoya City University Hospital Nagoya Aichi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuzuru","family":"Kamei","sequence":"additional","affiliation":[{"name":"Department of Plastic and Reconstructive Surgery Nagoya University Graduate School of Medicine Nagoya Aichi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tokunori","family":"Yamamoto","sequence":"additional","affiliation":[{"name":"Department of Urology Nagoya University Graduate School of Medicine Nagoya Aichi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masaaki","family":"Mizuno","sequence":"additional","affiliation":[{"name":"Departments of Department of Advanced Medicine Nagoya University Hospital Nagoya Aichi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Momokazu","family":"Gotoh","sequence":"additional","affiliation":[{"name":"Department of Urology Nagoya University Graduate School of Medicine Nagoya Aichi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,12]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/PL00004033"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.2217\/WHE.13.17"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.3143\/geriatrics.50.362"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s001200050064"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1089\/end.1997.11.273"},{"key":"e_1_2_8_7_1","unstructured":"LucasMG BedretdinovaD BerghmansLCet al.Guidelines on Urinary Incontinence; European Association of Urology2015.http:\/\/uroweb.org\/wp\u2010content\/uploads\/20\u2010Urinary\u2010Incontinence_LR1.pdf. 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