{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T04:14:39Z","timestamp":1791346479672,"version":"4.3.1"},"reference-count":36,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2021,1,5]],"date-time":"2021-01-05T00:00:00Z","timestamp":1609804800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J of Urology"],"published-print":{"date-parts":[[2021,4]]},"abstract":"<jats:sec><jats:title>Objectives<\/jats:title><jats:p>To investigate whether a response to hydrogen sulfide donors (GYY4137 and sodium hydrosulfide) and the endogenous hydrogen sulfide system (hydrogen sulfide level and expression of cysteine aminotransferase, cystathionine \u03b2\u2010synthase, cystathionine \u03b3\u2010lyase, and 3\u2010mercaptopyruvate sulfurtransferase) in the spontaneously hypertensive rat bladder differ with age, we compared the responses of hydrogen sulfide donors to micturition and bladder relaxation, and the endogenous hydrogen sulfide system in the bladder of 18\u2010week versus 12\u2010week\u2010old spontaneously hypertensive rats.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>GYY4137 was intravesically administered and cystometry was performed in anesthetized rats. The responses of sodium hydrosulfide were evaluated in carbachol\u2010mediated precontracted bladder strips. Bladder hydrogen sulfide levels and expression levels of each enzyme were investigated using the methylene blue method and Western blotting, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>GYY4137 treatment significantly prolonged intercontraction intervals only in 12\u2010week\u2010old rats. Sodium hydrosulfide\u2010induced bladder relaxation was significantly attenuated in the strips of 18\u2010week\u2010old rats compared with that in 12\u2010week\u2010old rats. In the bladder dome, significant increases in hydrogen sulfide levels and in the expression of cystathionine \u03b2\u2010synthase, 3\u2010mercaptopyruvate sulfurtransferase, and cysteine aminotransferase were observed in 18\u2010week\u2010old rats compared with 12\u2010week\u2010old rats. However, cystathionine \u03b3\u2010lyase bands were not detected in bladder tissues of either group.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Bladder relaxation induced by hydrogen sulfide may be attenuated in spontaneously hypertensive rats in an age\u2010dependent manner.<\/jats:p><\/jats:sec>","DOI":"10.1111\/iju.14478","type":"journal-article","created":{"date-parts":[[2021,1,6]],"date-time":"2021-01-06T22:43:46Z","timestamp":1609973026000},"page":"459-465","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Age\u2010related differences in responses to hydrogen sulfide in the bladder of spontaneously hypertensive rats"],"prefix":"10.1111","volume":"28","author":[{"given":"Suo","family":"Zou","sequence":"first","affiliation":[{"name":"Departments of Department of Pharmacology Kochi Medical School Kochi University Nankoku Kochi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takahiro","family":"Shimizu","sequence":"additional","affiliation":[{"name":"Departments of Department of Pharmacology Kochi Medical School Kochi University Nankoku Kochi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masaki","family":"Yamamoto","sequence":"additional","affiliation":[{"name":"Departments of Department of Pharmacology Kochi Medical School Kochi University Nankoku Kochi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shogo","family":"Shimizu","sequence":"additional","affiliation":[{"name":"Departments of Department of Pharmacology Kochi Medical School Kochi University Nankoku Kochi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Youichirou","family":"Higashi","sequence":"additional","affiliation":[{"name":"Departments of Department of Pharmacology Kochi Medical School Kochi University Nankoku Kochi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Karashima","sequence":"additional","affiliation":[{"name":"Department of Urology Kochi Medical School Kochi University Nankoku Kochi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Motoaki","family":"Saito","sequence":"additional","affiliation":[{"name":"Departments of Department of Pharmacology Kochi Medical School Kochi University Nankoku Kochi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,1,5]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.niox.2014.11.010"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.niox.2014.01.002"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1162667"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2014.5869"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/nau.23788"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.1998.275.4.R1366"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.5213\/inj.2011.15.4.192"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0706630"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11255-019-02222-1"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1538\/expanim.53.67"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep03822"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464-410X.2011.10814.x"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1124\/pr.117.014050"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.2012.02127.x"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1097\/00005344-199403000-00011"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/20.21.6008"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2012.10.006"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1097\/FJC.0000000000000394"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1021\/acschemneuro.8b00533"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.STR.0000181077.84981.11"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11906-003-0013-1"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00280.2014"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1743-6109.2009.01249.x"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.urology.2011.12.032"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1097\/HJH.0000000000000746"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/nau.22405"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00097.2017"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2003.11.081"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00109-014-1227-1"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1080\/10641963.2017.1313847"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/mnfr.201700596"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00282.2016"},{"key":"e_1_2_8_34_1","article-title":"Changes in the vasoactive effects of nitric oxide, hydrogen sulfide and the structure of the rat thoracic aorta: the role of age and essential hypertension","volume":"69","author":"Berenyiova A","year":"2018","journal-title":"J. Physiol. Pharmacol."},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-4889(02)00309-9"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/nau.21213"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00331.2004"}],"container-title":["International Journal of Urology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/iju.14478","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/iju.14478","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/iju.14478","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,1]],"date-time":"2023-09-01T18:20:28Z","timestamp":1693592428000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/iju.14478"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,1,5]]},"references-count":36,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2021,4]]}},"alternative-id":["10.1111\/iju.14478"],"URL":"https:\/\/doi.org\/10.1111\/iju.14478","archive":["Portico"],"relation":{},"ISSN":["0919-8172","1442-2042"],"issn-type":[{"value":"0919-8172","type":"print"},{"value":"1442-2042","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,1,5]]},"assertion":[{"value":"2020-07-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-11-29","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-01-05","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}