{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T20:15:53Z","timestamp":1780344953168,"version":"3.54.1"},"reference-count":31,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2021,4,2]],"date-time":"2021-04-02T00:00:00Z","timestamp":1617321600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["JP18fk0108019"],"award-info":[{"award-number":["JP18fk0108019"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J of Urology"],"published-print":{"date-parts":[[2021,6]]},"abstract":"<jats:sec><jats:title>Objectives<\/jats:title><jats:p>To compare antibiotic susceptibilities between chromosomal and plasmid <jats:italic>bla<\/jats:italic><jats:sub>CTX\u2010M\u201015<\/jats:sub> locations in urinary tract infection\u2010causing extended\u2010spectrum \u03b2\u2010lactamases\u2010producing <jats:italic>Escherichia<\/jats:italic> <jats:italic>coli<\/jats:italic> <jats:italic>bla<\/jats:italic><jats:sub>CTX\u2010M\u201015<\/jats:sub> isolated in Indonesia.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A total of 84 strains identified as extended\u2010spectrum \u03b2\u2010lactamases\u2010producing <jats:italic>E. coli<\/jats:italic> were isolated from patients with urinary tract infection in Indonesia in 2015. Antimicrobial susceptibility tests were performed on these strains using 18 antibiotics, and extended\u2010spectrum \u03b2\u2010lactamase <jats:italic>bla<\/jats:italic> genes were detected by polymerase chain reaction. Gene localization of <jats:italic>bla<\/jats:italic><jats:sub>CTX\u2010M\u201015<\/jats:sub>\u2010positive strains was confirmed by Southern blot hybridization, and epidemiological typing was conducted using multilocus sequence typing.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Of 54 strains harboring the <jats:italic>bla<\/jats:italic><jats:sub>CTX\u2010M\u201015<\/jats:sub> gene, 27 showed localization on chromosome, 20 on plasmid, and seven on chromosome and plasmid. Most multilocus sequence typing sequence types of the 27 strains with chromosomal <jats:italic>bla<\/jats:italic><jats:sub>CTX\u2010M\u201015<\/jats:sub> were ST405 (25.9%) and ST131 (22.2%) strains, whereas the 20 strains with plasmid\u2010<jats:italic>bla<\/jats:italic><jats:sub>CTX\u2010M\u201015<\/jats:sub> were mostly ST410 (55.0%).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Extended\u2010spectrum \u03b2\u2010lactamases\u2010producing <jats:italic>E<\/jats:italic>. <jats:italic>coli<\/jats:italic> <jats:italic>bla<\/jats:italic><jats:sub>CTX\u2010M\u201015<\/jats:sub> with plasmid genes show significantly higher resistant rates against piperacillin\u2010tazobactam but lower resistant rates against chloramphenicol compared to chromosomal strains in Indonesian patients with urinary tract infection. Mechanistic investigations will be necessary to advance our knowledge of antimicrobial resistance in urinary tract infection.<\/jats:p><\/jats:sec>","DOI":"10.1111\/iju.14498","type":"journal-article","created":{"date-parts":[[2021,4,3]],"date-time":"2021-04-03T06:13:45Z","timestamp":1617430425000},"page":"623-628","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Differential effects of chromosome and plasmid <i>bla<\/i><sub>CTX\u2010M\u201015<\/sub> genes on antibiotic susceptibilities in extended\u2010spectrum beta\u2010lactamase\u2010producing <i>Escherichia coli<\/i> isolates from patients with urinary tract infection"],"prefix":"10.1111","volume":"28","author":[{"given":"Young\u2010min","family":"Yang","sequence":"first","affiliation":[{"name":"Division of Urology Department of Organ Therapeutics Faculty of Medicine Kobe University Graduate School of Medicine Kobe Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kayo","family":"Osawa","sequence":"additional","affiliation":[{"name":"Division of Biophysics Kobe University Graduate School of Health Sciences Kobe Japan"},{"name":"Department of Medical Technology Kobe Tokiwa University Kobe Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koichi","family":"Kitagawa","sequence":"additional","affiliation":[{"name":"Department of Public Health Kobe University Graduate School of Health Sciences Kobe Japan"},{"name":"Department of Advanced Medical Science Kobe University Graduate School of Science, Technology and Innovation Kobe Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samiko","family":"Hosoya","sequence":"additional","affiliation":[{"name":"Department of Public Health Kobe University Graduate School of Health Sciences Kobe Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reo","family":"Onishi","sequence":"additional","affiliation":[{"name":"Department of Public Health Kobe University Graduate School of Health Sciences Kobe Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aya","family":"Ishii","sequence":"additional","affiliation":[{"name":"Department of Public Health Kobe University Graduate School of Health Sciences Kobe Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshiro","family":"Shirakawa","sequence":"additional","affiliation":[{"name":"Department of Public Health Kobe University Graduate School of Health Sciences Kobe Japan"},{"name":"Department of Advanced Medical Science Kobe University Graduate School of Science, Technology and Innovation Kobe Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Itaru","family":"Hirai","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology School of Health Sciences Faculty of Medicine University of the Ryukyus Okinawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kuntaman","family":"Kuntaman","sequence":"additional","affiliation":[{"name":"Department of Microbiology Faculty of Medicine Airlangga University Surabaya Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroshi","family":"Tanimoto","sequence":"additional","affiliation":[{"name":"Department of Public Health Kobe University Graduate School of Health Sciences Kobe Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katsumi","family":"Shigemura","sequence":"additional","affiliation":[{"name":"Division of Urology Department of Organ Therapeutics Faculty of Medicine Kobe University Graduate School of Medicine Kobe Japan"},{"name":"Department of Public Health Kobe University Graduate School of Health Sciences Kobe Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masato","family":"Fujisawa","sequence":"additional","affiliation":[{"name":"Division of Urology Department of Organ Therapeutics Faculty of Medicine Kobe University Graduate School of Medicine Kobe Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,4,2]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkm464"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0011202"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plasmid.2013.03.003"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.34.6.1271"},{"key":"e_1_2_8_6_1","first-page":"1","article-title":"Study on blaCTX\u2010M type of substrate specificity \u03b2\u2010lactamase\u2010producing Eschericia coli in Ehime Prefectural Central Hospital","volume":"68","author":"Kitao T","year":"2019","journal-title":"Med. 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