{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,13]],"date-time":"2026-09-13T23:40:40Z","timestamp":1789342840418,"version":"build-2803163510"},"reference-count":27,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2021,5,8]],"date-time":"2021-05-08T00:00:00Z","timestamp":1620432000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J of Urology"],"published-print":{"date-parts":[[2021,8]]},"abstract":"<jats:sec><jats:title>Objectives<\/jats:title><jats:p>To explore the expression of cytoskeletal and cell proliferation proteins in urothelial cells of patients diagnosed with various clinical subtypes of interstitial cystitis\/bladder pain syndrome.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Biopsy specimens from 85 interstitial cystitis\/bladder pain syndrome patients were classified according to findings on cystoscopy. Cytokeratins and cell proliferation proteins detected in the specimens were evaluated with immunofluorescence staining and quantified with western blotting. A total of 22 patients diagnosed with pure stress urinary incontinence were enrolled as controls.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Interstitial cystitis\/bladder pain syndrome patients with Hunner\u2019s lesion and with grade\u00a03 glomerulation hemorrhage had smaller bladder capacities than the other interstitial cystitis\/bladder pain syndrome patients without Hunner\u2019s lesion. Diminished expression of CK14, CK20, cell proliferation protein tumor protein\u00a063, sonic hedgehog, and fibroblast growth factor receptors\u00a03 and 4, and increased expression of CK5 and BCL2\u2010associated X protein were observed in biopsy specimens from patients with Hunner\u2019s lesion compared with those from patients without Hunner\u2019s lesion and controls. In the patients with grade\u00a03 glomerulation hemorrhage, lower expression levels of urothelial CK20, tumor protein\u00a063 and fibroblast growth factor receptor\u00a04, and lower expression of CK5 and BCL2\u2010associated X\u00a0protein were detected compared with other types of NHIC.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>A diminished expression of proliferation proteins tumor protein\u00a063 and the mature urothelium marker CK20, and increased expression of the immature marker CK5 in specimens from both Hunner\u2019s lesion and grade\u00a03 glomerulation hemorrhage patients can be observed. The urothelium of patients with interstitial cystitis\/bladder pain syndrome might be in a state of persistent or chronic injury that could relate to the limited expression of cell proliferation proteins.<\/jats:p><\/jats:sec>","DOI":"10.1111\/iju.14585","type":"journal-article","created":{"date-parts":[[2021,5,9]],"date-time":"2021-05-09T08:27:18Z","timestamp":1620548838000},"page":"823-830","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Decreased urothelial cytoskeleton and cell proliferation protein expression suggest interstitial cystitis\/bladder pain syndrome patients with Hunner\u2019s lesion and grade\u00a03 glomerulation might be different from other types of patients"],"prefix":"10.1111","volume":"28","author":[{"given":"Jia\u2010Fong","family":"Jhang","sequence":"first","affiliation":[{"name":"Department of Urology Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Tzu Chi University 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