{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,23]],"date-time":"2026-04-23T20:14:27Z","timestamp":1776975267562,"version":"3.51.4"},"reference-count":16,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2022,1,31]],"date-time":"2022-01-31T00:00:00Z","timestamp":1643587200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["16K11059"],"award-info":[{"award-number":["16K11059"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["19K18596"],"award-info":[{"award-number":["19K18596"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J of Urology"],"published-print":{"date-parts":[[2022,5]]},"abstract":"<jats:sec><jats:title>Objectives<\/jats:title><jats:p>This study was carried out to identify biomarkers that distinguish Hunner\u2010type interstitial cystitis from non\u2010Hunner\u2010type interstitial cystitis patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Total ribonucleic acid was purified from 212 punch biopsy specimens of 89 individuals who were diagnosed as interstitial cystitis\/bladder pain syndrome. To examine the expression profile of patients\u2019 bladder specimens, 68 urothelial master transcription factors and nine known markers (E\u2010cadherin, cytokeratins, uroplakins and sonic hedgehog) were selected. To classify the biopsy samples, principal component analysis was carried out. A decision tree algorithm was adopted to identify critical determinants, in which 102 and 116 bladder specimens were used for learning and validation, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Principal component analysis segregated tissues from Hunner\u2010type and non\u2010Hunner\u2010type interstitial cystitis specimens in principal component axes\u20092 and 4. Principal components 2 and 4 contained urothelial stem\/progenitor transcription factors and cytokeratins, respectively. A decision tree identified <jats:italic>KRT20<\/jats:italic>, <jats:italic>BATF<\/jats:italic> and <jats:italic>TP63<\/jats:italic> to classify non\u2010Hunner\u2010type and Hunner\u2010type interstitial cystitis specimens. <jats:italic>KRT20<\/jats:italic> was lower in tissues from Hunner\u2010type compared with non\u2010Hunner\u2010type interstitial cystitis specimens (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.001). <jats:italic>TP63<\/jats:italic> was lower in Hunner\u2019s lesions compared with adjacent mucosa from Hunner\u2010type interstitial cystitis patients (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.001). Blinded validation using additional biopsy specimens verified that the decision tree showed fairly precise concordance with cystoscopic diagnosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p><jats:italic>KRT20<\/jats:italic>, <jats:italic>BATF<\/jats:italic> and <jats:italic>TP63<\/jats:italic> were identified as biologically relevant biomarkers to classify tissues from interstitial cystitis\/bladder pain syndrome specimens. The biologically explainable determinants could contribute to defining the elusive interstitial cystitis\/bladder pain syndrome pathogenesis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/iju.14795","type":"journal-article","created":{"date-parts":[[2022,2,1]],"date-time":"2022-02-01T02:20:00Z","timestamp":1643682000000},"page":"406-412","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Supervised machine learning algorithm identified <i>KRT20<\/i>, <i>BATF<\/i> and <i>TP63<\/i> as biologically relevant biomarkers for bladder biopsy specimens from interstitial cystitis\/bladder pain syndrome patients"],"prefix":"10.1111","volume":"29","author":[{"given":"Tomohiko","family":"Kamasako","sequence":"first","affiliation":[{"name":"Department of Urology Continence Center Dokkyo Medical University  Tochigi 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