{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,16]],"date-time":"2026-02-16T09:00:55Z","timestamp":1771232455067,"version":"3.50.1"},"reference-count":30,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2022,4,5]],"date-time":"2022-04-05T00:00:00Z","timestamp":1649116800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002701","name":"Ministry of Education","doi-asserted-by":"publisher","award":["NRF\u20102018R1D1A1A02085556"],"award-info":[{"award-number":["NRF\u20102018R1D1A1A02085556"]}],"id":[{"id":"10.13039\/501100002701","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J of Urology"],"published-print":{"date-parts":[[2022,7]]},"abstract":"<jats:sec><jats:title>Objectives<\/jats:title><jats:p>To determine the anti\u2010fibrotic effects of Wnt\/\u03b2\u2010catenin signaling inhibitors on urethral stricture.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Human fibroblasts were exposed to transforming growth factor beta 1 combined with various concentrations of Wnt\/\u03b2\u2010catenin inhibitors (ICG\u2010001, IWR\u20101, and PRI\u2010724), and cell proliferation and migration were evaluated. Urethral fibrosis was induced in male Sprague\u2013Dawley rats by urethral injection of transforming growth factor beta 1 and co\u2010treatement with inhibitors. Urethral tissues were harvested 2\u2009weeks after the injection. The messenger ribonucleic acid and protein expression was examined for fibrosis markers Axin\u20101, collagen type 1, alpha smooth muscle actin, and \u03b2\u2010catenin. Histological analysis of fibrosis and collagen deposition was also performed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Cell migration was ameliorated by ICG\u2010001 and PRI\u2010724. Protein and messenger ribonucleic acid expression of collagen type 1 and alpha smooth muscle actin in transforming growth factor beta 1\u2010treated fibroblasts decreased in a concentration\u2010dependent manner with the ICG\u2010001 and PRI\u2010724 treatments (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05). However, there were no significant changes with the IWR\u20101 treatment. Collagen type I and alpha smooth muscle actin messenger ribonucleic acid and protein expression were both significantly increased in the urethral tissues of rats with transforming growth factor beta 1\u2010induced urethral fibrosis. Rats co\u2010treated with ICG\u2010001 or PRI\u2010724 showed relatively mild fibrosis and significantly reduced collagen type I and alpha smooth muscle actin messenger ribonucleic acid and protein expression (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>ICG\u2010001 and PRI\u2010724 significantly ameliorated urethral fibrosis induced by transforming growth factor beta 1 in rats. These results suggest that ICG\u2010001 and PRI\u2010724 can be developed as therapeutics for treating urethral stricture.<\/jats:p><\/jats:sec>","DOI":"10.1111\/iju.14884","type":"journal-article","created":{"date-parts":[[2022,4,5]],"date-time":"2022-04-05T08:06:29Z","timestamp":1649145989000},"page":"764-771","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Prevention of urethral fibrosis induced by transforming growth factor beta 1 using selective Wnt\/\u03b2\u2010catenin signaling inhibitors in a rat model"],"prefix":"10.1111","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9421-9596","authenticated-orcid":false,"given":"Kyung Hwa","family":"Choi","sequence":"first","affiliation":[{"name":"Department of Urology, CHA Bundang Medical Center CHA University School of Medicine  Seongnam Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3237-6304","authenticated-orcid":false,"given":"Dae Keun","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Urology, CHA Fertility Center Seoul Station CHA University School of Medicine  Seoul Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A Ram","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Dermatology, School of Medicine CHA University School of Medicine  Pocheon Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9959-3488","authenticated-orcid":false,"given":"Seung\u2010Ryeol","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Urology, CHA Bundang Medical Center CHA University School of Medicine  Seongnam Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,4,5]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464-410X.2007.06904.x"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s002400000119"},{"key":"e_1_2_13_4_1","doi-asserted-by":"publisher","DOI":"10.1513\/pats.201203-023AW"},{"key":"e_1_2_13_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0076808"},{"key":"e_1_2_13_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms160921911"},{"key":"e_1_2_13_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11010-014-2104-5"},{"key":"e_1_2_13_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2015.02.014"},{"key":"e_1_2_13_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms1734"},{"key":"e_1_2_13_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1001520107"},{"key":"e_1_2_13_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2017.12.001"},{"key":"e_1_2_13_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2016.12.003"},{"key":"e_1_2_13_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-00282-w"},{"key":"e_1_2_13_14_1","doi-asserted-by":"publisher","DOI":"10.1080\/01902148.2019.1638466"},{"key":"e_1_2_13_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2007.11.036"},{"key":"e_1_2_13_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2008.08.017"},{"key":"e_1_2_13_17_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0404875101"},{"key":"e_1_2_13_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/cas.12471"},{"key":"e_1_2_13_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/jm201510p"},{"key":"e_1_2_13_20_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0075010"},{"key":"e_1_2_13_21_1","first-page":"129","article-title":"Transforming growth factor\u2010beta1 circulates in normal human plasma and is unchanged in advanced metastatic breast cancer","volume":"1","author":"Wakefield LM","year":"1995","journal-title":"Clin. 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