{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,10]],"date-time":"2026-04-10T07:53:03Z","timestamp":1775807583423,"version":"3.50.1"},"reference-count":33,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2022,5,18]],"date-time":"2022-05-18T00:00:00Z","timestamp":1652832000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J of Urology"],"published-print":{"date-parts":[[2022,8]]},"abstract":"<jats:sec><jats:title>Objectives<\/jats:title><jats:p>To investigate the effects of pretreatment with 5\u2010aminolevulinic acid hydrochloride combined with sodium ferrous citrate on bladder dysfunction in cyclophosphamide\u2010induced hemorrhagic cystitis in rats.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Male Wistar rats (340\u2013460\u2009g) were pretreated with vehicle or with 5\u2010aminolevulinic acid hydrochloride combined with sodium ferrous citrate (100\/157 or 300\/471\u2009mg\/kg\/day, po) once daily for 7\u2009days before cystometry. Saline or cyclophosphamide (150\u2009mg\/kg, ip) was administered 2\u2009days before cystometry. Cystometry was performed under urethane anesthesia (0.8\u2009g\/kg, ip) via a catheter inserted into the bladder. After cystometry, bladder tissues were collected to perform hematoxylin and eosin staining for pathological evaluation (neutrophil infiltration, edema, and bleeding scores), and for enzyme\u2010linked immunosorbent assay and real\u2010time polymerase chain reaction for investigating tissue levels of myeloperoxidase, and mRNA levels of haem oxygenase\u20101 as a cytoprotective molecule.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Compared to controls, cyclophosphamide induced a shorter intercontraction interval, lower bladder compliance, increased number of non\u2010voiding contractions, and increased pathological scores and myeloperoxidase expression in the bladder. Pretreatment with 5\u2010aminolevulinic acid hydrochloride combined with sodium ferrous citrate (300\/471\u2009mg\/kg\/day) significantly improved cyclophosphamide\u2010induced intercontraction interval shortening and increases in number of non\u2010voiding contractions and neutrophil infiltration\/bleeding scores and enhanced haem oxygenase\u20101 expression in the bladder. In addition, cyclophosphamide\u2010induced decreases in bladder compliance and increases in myeloperoxidase were not detected with 5\u2010aminolevulinic acid hydrochloride combined with sodium ferrous citrate pretreatment.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Pretreatment with 5\u2010aminolevulinic acid expects protective effects on bladder dysfunction in cyclophosphamide\u2010induced hemorrhagic cystitis by improving inflammatory changes in bladder tissues perhaps via up\u2010regulation of haem oxygenase\u20101.<\/jats:p><\/jats:sec>","DOI":"10.1111\/iju.14928","type":"journal-article","created":{"date-parts":[[2022,5,18]],"date-time":"2022-05-18T07:43:57Z","timestamp":1652859837000},"page":"897-904","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["<scp>5\u2010Aminolevulinic<\/scp> acid has the potential to prevent bladder dysfunction in cyclophosphamide\u2010induced hemorrhagic cystitis"],"prefix":"10.1111","volume":"29","author":[{"given":"Takahira","family":"Kuno","sequence":"first","affiliation":[{"name":"Department of Urology, Kochi Medical School Kochi University  Nankoku Kochi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1555-2284","authenticated-orcid":false,"given":"Takahiro","family":"Shimizu","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Kochi Medical School Kochi University  Nankoku Kochi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chiaki","family":"Kawada","sequence":"additional","affiliation":[{"name":"Department of Urology, Kochi Medical School Kochi University  Nankoku Kochi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Atsushi","family":"Kurabayashi","sequence":"additional","affiliation":[{"name":"Department of Pathology, Kochi Medical School Kochi University  Nankoku Kochi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suo","family":"Zou","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Kochi Medical School Kochi University  Nankoku Kochi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroki","family":"Mogawa","sequence":"additional","affiliation":[{"name":"Institute for Laboratory Animal Research, Kochi Medical School Kochi University  Nankoku Kochi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masayuki","family":"Tsuda","sequence":"additional","affiliation":[{"name":"Institute for Laboratory Animal Research, Kochi Medical School Kochi University  Nankoku Kochi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2962-1607","authenticated-orcid":false,"given":"Motoaki","family":"Saito","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Kochi Medical School Kochi University  Nankoku Kochi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keiji","family":"Inoue","sequence":"additional","affiliation":[{"name":"Department of Urology, Kochi Medical School Kochi University  Nankoku Kochi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,5,18]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00210-017-1361-8"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.27053"},{"key":"e_1_2_13_4_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.1991.9.11.2016"},{"key":"e_1_2_13_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/bju.12291"},{"key":"e_1_2_13_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40360-016-0108-3"},{"key":"e_1_2_13_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2010.11.029"},{"key":"e_1_2_13_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2015.11.034"},{"key":"e_1_2_13_9_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M108362200"},{"key":"e_1_2_13_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tox.2017.07.016"},{"key":"e_1_2_13_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/nau.24313"},{"key":"e_1_2_13_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2018.05.009"},{"key":"e_1_2_13_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2017.03.018"},{"key":"e_1_2_13_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.2010.00873.x"},{"key":"e_1_2_13_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/acer.14117"},{"key":"e_1_2_13_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jphs.2016.08.007"},{"key":"e_1_2_13_17_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00450.2015"},{"key":"e_1_2_13_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464-410X.2008.07662.x"},{"key":"e_1_2_13_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-86886-9"},{"key":"e_1_2_13_20_1","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_13_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00210-010-0555-0"},{"key":"e_1_2_13_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/nau.23860"},{"key":"e_1_2_13_23_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00574.2012"},{"key":"e_1_2_13_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/nau.24241"},{"key":"e_1_2_13_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/nau.24363"},{"key":"e_1_2_13_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpain.2007.09.004"},{"key":"e_1_2_13_27_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnins.2011.00020"},{"key":"e_1_2_13_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11255-019-02128-y"},{"key":"e_1_2_13_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1442-2042.2006.01536.x"},{"key":"e_1_2_13_30_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.172.3.1839"},{"key":"e_1_2_13_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.abb.2014.09.005"},{"key":"e_1_2_13_32_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.175.7.4408"},{"key":"e_1_2_13_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2014.02.003"},{"key":"e_1_2_13_34_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.19.11783"}],"container-title":["International Journal of Urology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/iju.14928","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/iju.14928","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/iju.14928","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,25]],"date-time":"2023-08-25T07:36:26Z","timestamp":1692948986000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/iju.14928"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,5,18]]},"references-count":33,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2022,8]]}},"alternative-id":["10.1111\/iju.14928"],"URL":"https:\/\/doi.org\/10.1111\/iju.14928","archive":["Portico"],"relation":{},"ISSN":["0919-8172","1442-2042"],"issn-type":[{"value":"0919-8172","type":"print"},{"value":"1442-2042","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,5,18]]},"assertion":[{"value":"2022-01-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-04-25","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-05-18","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}