{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T23:02:27Z","timestamp":1791414147100,"version":"4.3.3"},"reference-count":27,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2025,7,29]],"date-time":"2025-07-29T00:00:00Z","timestamp":1753747200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,7,29]],"date-time":"2025-07-29T00:00:00Z","timestamp":1753747200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J of Urology"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>The progressive decline in birth rates due to delayed marriage has become a significant issue in developed countries, with male spermatogenetic capabilities gradually declining with age. This study evaluated the effectiveness of a silicon\u2010based agent (Si\u2010based) that efficiently generates hydrogen in the body to improve spermatogenic function in aged mice.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Eighty\u2010five\u2010week\u2010old (aged) C57BL\/6J mice were administered either a normal diet or a diet supplemented with a Si\u2010based agent. Sperm analysis, histopathology, immunohistochemistry, immunofluorescence, and immunoblotting were performed to assess spermatogenic function, markers of oxidative stress, and activation of the Kelch\u2010like ECH\u2010associated protein 1 (KEAP1)\/nuclear factor erythroid 2\u2010related factor 2 (NRF2) pathway.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The Si\u2010based agent significantly increased sperm count and velocity (curvilinear and straight\u2010line velocity), reduced the proportion of abnormal seminiferous tubules, and improved testicular histology in aged mice (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.05). Furthermore, the Si\u2010based agent decreased the expression of oxidative stress markers and modulated the KEAP1\/NRF2 pathway by reducing the expression of KEAP1 and increasing NRF2 nuclear translocation, leading to the upregulation of the antioxidant genes\u2014heme oxygenase (HMOX)\u20101 and NAD(P)H quinone oxidoreductase (NQO\u20101).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>These findings suggest that hydrogen delivered through a Si\u2010based agent could be a promising therapeutic approach for age\u2010related male infertility. This warrants further studies to explore its potential in clinical settings.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/iju.70189","type":"journal-article","created":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T06:06:07Z","timestamp":1753855567000},"page":"1696-1702","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Silicon\u2010Based Agent Attenuates Age\u2010Related Spermatogenesis Disorder"],"prefix":"10.1111","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0564-6404","authenticated-orcid":false,"given":"Sohei","family":"Kuribayashi","sequence":"first","affiliation":[{"name":"Department of Urology Osaka University Graduate School of Medicine  Suita Japan"},{"name":"Research Institute for Microbial Diseases Osaka University  Suita Japan"},{"name":"Department of Urology Glickman Urological &amp; Kidney Institute, Cleveland Clinic Foundation  Cleveland Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1336-4316","authenticated-orcid":false,"given":"Shinichiro","family":"Fukuhara","sequence":"additional","affiliation":[{"name":"Department of Urology Osaka University Graduate School of Medicine  Suita Japan"},{"name":"Department of Urology JCHO Osaka Hospital  Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-8421-7911","authenticated-orcid":false,"given":"Hiroaki","family":"Kitakaze","sequence":"additional","affiliation":[{"name":"Department of Urology Osaka University Graduate School of Medicine  Suita Japan"},{"name":"Research Institute for Microbial Diseases Osaka University  Suita Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4493-8917","authenticated-orcid":false,"given":"Go","family":"Tsujimura","sequence":"additional","affiliation":[{"name":"Department of Urology Osaka University Graduate School of Medicine  Suita Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5403-5362","authenticated-orcid":false,"given":"Takahiro","family":"Imanaka","sequence":"additional","affiliation":[{"name":"Department of Urology Osaka University Graduate School of Medicine  Suita Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9131-2867","authenticated-orcid":false,"given":"Norichika","family":"Ueda","sequence":"additional","affiliation":[{"name":"Department of Urology Osaka University Graduate School of Medicine  Suita Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1137-1846","authenticated-orcid":false,"given":"Kentaro","family":"Takezawa","sequence":"additional","affiliation":[{"name":"Department of Urology Osaka University Graduate School of Medicine  Suita Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hikaru","family":"Kobayashi","sequence":"additional","affiliation":[{"name":"SANKEN (Institute of Scientific and Industrial Research), Osaka University  Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8782-9852","authenticated-orcid":false,"given":"Ryoichi","family":"Imamura","sequence":"additional","affiliation":[{"name":"Department of Urology Osaka University Graduate School of Medicine  Suita Japan"},{"name":"Department of Urology Nagasaki University Graduate School of Biomedical Science  Nagasaki Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6522-6233","authenticated-orcid":false,"given":"Norio","family":"Nonomura","sequence":"additional","affiliation":[{"name":"Department of Urology Osaka University Graduate School of Medicine  Suita Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,7,29]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/dmaa046"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2013.05.046"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2021.101867"},{"key":"e_1_2_11_5_1","article-title":"The Mechanistic Perspective of Bilobetin Protective Effects Against Cisplatin\u2010Induced Testicular Toxicity: Role of Nrf\u20102\/Keap\u20101 Signaling, Inflammation and Apoptosis","volume":"10","author":"Negm W. 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