{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T15:30:40Z","timestamp":1787671840146,"version":"build-2736575974"},"reference-count":30,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,3,5]],"date-time":"2026-03-05T00:00:00Z","timestamp":1772668800000},"content-version":"vor","delay-in-days":4,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J of Urology"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To evaluate the effect of tertiary lymphoid structure (TLS)\u2010like lymphocyte aggregates in radical cystectomy (RC) specimens on the survival outcomes of patients with bladder cancer.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We included 110 patients who underwent RC for urothelial carcinoma of the bladder. Hematoxylin and eosin\u2010stained slides of RC specimens were evaluated for immune\u2010related pathological results including the presence of TLS\u2010like lymphocyte aggregates. Survival outcomes between groups were compared. The effects of various parameters on non\u2010urinary tract recurrence\u2010free survival (NUTRFS) were evaluated.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Among the 110 patients, TLS\u2010like lymphocyte aggregates were identified in 65 (59.1%). Immunohistochemical evaluation of TLS\u2010like lymphocyte aggregates in five selected patients using antibodies against CD4, CD8, and CD20 demonstrated that all lesions contained organized structures with T lymphocytes surrounding the B lymphocytes, consistent with TLSs. The median follow\u2010up duration was 39.8\u2009months (interquartile range, 14.7\u201379.3\u2009months). Analyses of overall survival, cancer\u2010specific survival, and NUTRFS showed that patients with TLS\u2010like lymphocyte aggregates had significantly better outcomes than those without such aggregates. In both univariable and multivariable Cox proportional hazards analyses for NUTRFS, the presence of TLSs remained an independent and statistically significant prognostic factor, along with pathological T stage and lymph node status (hazard ratio 0.25, 95% confidence interval 0.12\u20130.52), indicating a favorable prognosis in patients with TLS\u2010like lymphocyte aggregates.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The findings of this study suggest that the presence of TLS\u2010like lymphocyte aggregates observed on routinely used hematoxylin and eosin\u2010stained slides of RC specimens may be an important predictor of survival outcomes after RC.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/iju.70392","type":"journal-article","created":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T15:33:39Z","timestamp":1772984019000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Prognostic Significance of Tertiary Lymphoid Structure\u2010Like Lymphocyte Aggregates in Routine Histopathological Examination in Bladder Cancer"],"prefix":"10.1111","volume":"33","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5854-6258","authenticated-orcid":false,"given":"Takeshi","family":"Sano","sequence":"first","affiliation":[{"name":"Department of Urology and Andrology Kansai Medical University  Hirakata\u2010shi Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chisato","family":"Ohe","sequence":"additional","affiliation":[{"name":"Department of Urology and Andrology Kansai Medical University  Hirakata\u2010shi Osaka Japan"},{"name":"Department of Surgical Pathology, School of Medicine Hyogo Medical University  Nishinomiya Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yusuke","family":"Motoki","sequence":"additional","affiliation":[{"name":"Department of Urology and Andrology Kansai Medical University  Hirakata\u2010shi Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takahiro","family":"Nakamoto","sequence":"additional","affiliation":[{"name":"Department of Urology and Andrology Kansai Medical University  Hirakata\u2010shi Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Yoshida","sequence":"additional","affiliation":[{"name":"Department of Urology Osaka Saiseikai\u2010Noe Hospital  Joto\u2010ku Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0797-8525","authenticated-orcid":false,"given":"Junichi","family":"Ikeda","sequence":"additional","affiliation":[{"name":"Department of Urology and Andrology Kansai Medical University  Hirakata\u2010shi Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7404-0369","authenticated-orcid":false,"given":"Hisanori","family":"Taniguchi","sequence":"additional","affiliation":[{"name":"Department of Urology and Andrology Kansai Medical University  Hirakata\u2010shi Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masaaki","family":"Yanishi","sequence":"additional","affiliation":[{"name":"Department of Urology and Andrology Kansai Medical University  Hirakata\u2010shi Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hidefumi","family":"Kinoshita","sequence":"additional","affiliation":[{"name":"Department of Urology and Andrology Kansai Medical University  Hirakata\u2010shi Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,3,5]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21834"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(16)30512-8"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2001.19.3.666"},{"key":"e_1_2_12_5_1","doi-asserted-by":"crossref","first-page":"336","DOI":"10.1038\/s41571-024-00884-0","article-title":"A New Standard of Care for Advanced\u2010Stage Urothelial Carcinoma","volume":"21","author":"Sidaway P.","year":"2024","journal-title":"Nature Reviews Clinical Oncology"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40164-022-00277-y"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature21349"},{"key":"e_1_2_12_8_1","first-page":"323","article-title":"Histologic\u2010Based Tumor\u2010Associated Immune Cells Status in Clear Cell Renal Cell Carcinoma Correlates With Gene Signatures Related to Cancer Immunity and Clinical Outcomes","volume":"10","author":"Ohe C.","year":"2022","journal-title":"Biomedicine"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.abf9419"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10147-023-02386-y"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/cas.15976"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/oncolo\/oyad283"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41568-024-00728-0"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41416-024-02883-5"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1136\/jitc-2021-003883"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/cam4.70978"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41416-024-02598-7"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1080\/2162402X.2021.1915574"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.793964"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00262-024-03929-6"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1136\/jitc-2023-006667"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.648812"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2018.09.003"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.2015.145"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/cam4.5227"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00262-019-02407-8"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.1158\/2326-6066.CIR-18-0758"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464-410X.2009.09166.x"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0089259"},{"key":"e_1_2_12_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.annonc.2023.11.016"},{"key":"e_1_2_12_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcancer.2017.10.003"}],"container-title":["International Journal of Urology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/iju.70392","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/iju.70392","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/iju.70392","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T14:59:28Z","timestamp":1787669968000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/iju.70392"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3]]},"references-count":30,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2026,3]]}},"alternative-id":["10.1111\/iju.70392"],"URL":"https:\/\/doi.org\/10.1111\/iju.70392","archive":["Portico"],"relation":{},"ISSN":["0919-8172","1442-2042"],"issn-type":[{"value":"0919-8172","type":"print"},{"value":"1442-2042","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,3]]},"assertion":[{"value":"2025-11-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-02-17","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-03-05","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70392"}}