{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T09:54:12Z","timestamp":1781862852785,"version":"3.54.5"},"reference-count":16,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T00:00:00Z","timestamp":1779235200000},"content-version":"vor","delay-in-days":19,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP21K09428"],"award-info":[{"award-number":["JP21K09428"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J of Urology"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>To clarify the roles of bladder carbon monoxide (CO), an endogenous gasotransmitter, in regulation of the micturition, we investigated the effects of a CO\u2010releasing molecule (CORM3) on the micturition and the contractility of bladder tissues in rats. We also examined expression levels of heme oxygenase (HO)\u20101 and HO\u20102, enzymes related to CO biosynthesis, in the rat bladder.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      In urethane\u2010anesthetized (0.8\u2009g\/kg, ip) male Wistar rats, the effects of intravesically administered CORM3 (10\n                      <jats:sup>\u22128<\/jats:sup>\n                      , 10\n                      <jats:sup>\u22127<\/jats:sup>\n                      and 10\n                      <jats:sup>\u22126<\/jats:sup>\n                      \u2009M) on the micturition were investigated by continuous cystometry. The effects of CORM3 (1\u2009\u00d7\u200910\n                      <jats:sup>\u22128<\/jats:sup>\n                      \u20133\u2009\u00d7\u200910\n                      <jats:sup>\u22125<\/jats:sup>\n                      \u2009M) on the contractility of bladder strips pre\u2010contracted by carbachol (10\n                      <jats:sup>\u22125<\/jats:sup>\n                      \u2009M) were examined. Levels of mRNA of HO\u20101 and HO\u20102 in the bladder were investigated by real\u2010time polymerase chain reaction, and distribution of these isozymes in the rat bladder was immunohistochemically examined.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Intravesical administration of CORM3 (10\n                      <jats:sup>\u22126<\/jats:sup>\n                      \u2009M) significantly prolonged intercontraction intervals without affecting maximal voiding pressure, baseline pressure or threshold pressure. In bladder tissue strips pre\u2010contracted by carbachol, CORM3 exhibited a slight relaxation effect, although the difference was not statistically significant compared with the vehicle\u2010treated control group. In the rat bladder tissues, HO\u20101 and HO\u20102 mRNA were detected. Immunoreactivity of both isozymes was mainly detected in the mucosal and muscle layers in the bladder, and HO\u20102, but not HO\u20101, was colocalized with \u03b23\u2010tubulin, a neuronal marker.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Bladder CO has a possibility to inhibit the rat micturition independently of bladder smooth muscle relaxation and the rat bladder has a potential for endogenous CO production.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/iju.70513","type":"journal-article","created":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T23:59:38Z","timestamp":1779321578000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Possible Role of Bladder Carbon Monoxide in the Regulation of Micturition Reflex in Rats"],"prefix":"10.1111","volume":"33","author":[{"given":"Chisato","family":"Hamada","sequence":"first","affiliation":[{"name":"Department of Pharmacology Kochi Medical School, Kochi University  Nankoku Kochi Japan"},{"name":"Department of Legal Medicine Kochi Medical School, Kochi University  Nankoku Kochi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1555-2284","authenticated-orcid":false,"given":"Takahiro","family":"Shimizu","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Kochi Medical School, Kochi University  Nankoku Kochi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suo","family":"Zou","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Kochi Medical School, Kochi University  Nankoku Kochi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Youichirou","family":"Higashi","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Kochi Medical School, Kochi University  Nankoku Kochi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satoshi","family":"Fukata","sequence":"additional","affiliation":[{"name":"Department of Urology Kochi Medical School, Kochi University  Nankoku Kochi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4185-323X","authenticated-orcid":false,"given":"Nobutaka","family":"Shimizu","sequence":"additional","affiliation":[{"name":"Pelvic Floor Center, Kochi Medical School, Kochi University  Nankoku Kochi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junichi","family":"Furumiya","sequence":"additional","affiliation":[{"name":"Department of Legal Medicine Kochi Medical School, Kochi University  Nankoku Kochi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Motoaki","family":"Saito","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Kochi Medical School, Kochi University  Nankoku Kochi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,20]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbi.2020.108973"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/scisignal.268re2"},{"key":"e_1_2_13_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.12729"},{"key":"e_1_2_13_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2022.115008"},{"key":"e_1_2_13_6_1","doi-asserted-by":"publisher","DOI":"10.1124\/pr.57.4.3"},{"key":"e_1_2_13_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0700893"},{"key":"e_1_2_13_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0701645"},{"key":"e_1_2_13_9_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.3000410"},{"key":"e_1_2_13_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/nau.23788"},{"key":"e_1_2_13_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep43778"},{"key":"e_1_2_13_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jphs.2025.08.006"},{"key":"e_1_2_13_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0701222"},{"key":"e_1_2_13_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0705825"},{"key":"e_1_2_13_15_1","doi-asserted-by":"crossref","unstructured":"A.Verma D. J.Hirsch C. E.Glatt G. V.Ronnett andS. H.Snyder \u201cCarbon Monoxide: A Putative Neural Messenger. Science (1979) \u201d259 no.5093(1993):7678352 https:\/\/doi.org\/10.1126\/science.7678352.","DOI":"10.1126\/science.7678352"},{"key":"e_1_2_13_16_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.1993.265.4.g799"},{"key":"e_1_2_13_17_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2001.015750"}],"container-title":["International Journal of Urology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/iju.70513","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/iju.70513","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/iju.70513","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T09:24:44Z","timestamp":1781861084000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/iju.70513"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5]]},"references-count":16,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2026,5]]}},"alternative-id":["10.1111\/iju.70513"],"URL":"https:\/\/doi.org\/10.1111\/iju.70513","archive":["Portico"],"relation":{},"ISSN":["0919-8172","1442-2042"],"issn-type":[{"value":"0919-8172","type":"print"},{"value":"1442-2042","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5]]},"assertion":[{"value":"2026-01-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-13","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-20","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70513"}}