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Some of these proteins have been implicated in multidrug resistance (\n                    <jats:styled-content style=\"fixed-case\">MDR<\/jats:styled-content>\n                    ). In this study, we identified a putative\n                    <jats:styled-content style=\"fixed-case\">P<\/jats:styled-content>\n                    \u2010glycoprotein in the\n                    <jats:italic>\n                      <jats:styled-content style=\"fixed-case\">Ae<\/jats:styled-content>\n                      .\u2009aegypti\n                    <\/jats:italic>\n                    database based on its significantly high identity with\n                    <jats:italic>\n                      <jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>\n                      nopheles gambiae\n                    <\/jats:italic>\n                    ,\n                    <jats:italic>\n                      <jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>\n                      ulex quinquefasciatus,\n                      <jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>\n                      rosophila melanogaster\n                    <\/jats:italic>\n                    and human\n                    <jats:styled-content style=\"fixed-case\">P<\/jats:styled-content>\n                    \u2010gps. The basal\n                    <jats:styled-content style=\"fixed-case\">ATPase<\/jats:styled-content>\n                    activity of\n                    <jats:styled-content style=\"fixed-case\">ATP\u2010<\/jats:styled-content>\n                    binding cassette transporters in larvae was significantly increased in the presence of\n                    <jats:styled-content style=\"fixed-case\">MDR<\/jats:styled-content>\n                    modulators (verapamil and quinidine). An eightfold increase in\n                    <jats:italic>Ae.\u2009aegypti P\u2010gp<\/jats:italic>\n                    (\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>AaegP<\/jats:italic>\n                    <\/jats:styled-content>\n                    <jats:italic>\u2010gp<\/jats:italic>\n                    ) gene expression was detected in temephos\u2010treated larvae as determined by quantitative\n                    <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>\n                    . To analyse the potential role of\n                    <jats:styled-content style=\"fixed-case\">AaegP<\/jats:styled-content>\n                    \u2010gp in insecticide efflux, a temephos larvicide assay was performed in the presence of verapamil. The results showed an increase of 24% in temephos toxicity, which is in agreement with the efflux reversing effect.\n                    <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\n                    interference (RNAi)\u2010mediated silencing of the\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>AaegP<\/jats:italic>\n                    <\/jats:styled-content>\n                    <jats:italic>\u2010gp<\/jats:italic>\n                    gene caused a significant increase in temephos toxicity (57%). In conclusion, we have demonstrated for the first time in insects that insecticide\u2010induced\n                    <jats:italic>\n                      <jats:styled-content style=\"fixed-case\">P<\/jats:styled-content>\n                      \u2010gp\n                    <\/jats:italic>\n                    expression can be involved in the modulation of insecticide efflux.\n                  <\/jats:p>","DOI":"10.1111\/imb.12052","type":"journal-article","created":{"date-parts":[[2013,8,27]],"date-time":"2013-08-27T08:04:38Z","timestamp":1377590678000},"page":"648-658","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":74,"title":["Silencing of\n                    <i>\n                      <scp>P<\/scp>\n                      \u2010glycoprotein\n                    <\/i>\n                    increases mortality in temephos\u2010treated\n                    <i>\n                      <scp>A<\/scp>\n                      edes aegypti\n                    <\/i>\n                    larvae"],"prefix":"10.1111","volume":"22","author":[{"given":"J.","family":"Figueira\u2010Mansur","sequence":"first","affiliation":[{"name":"Universidade Federal do Rio de Janeiro Instituto de Qu\u00edmica  Rio de Janeiro RJ Brazil"},{"name":"Universidade Federal do Rio de Janeiro Instituto de Bioqu\u00edmica M\u00e9dica Programa de Biologia Molecular e Biotecnologia  Rio de Janeiro RJ Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Ferreira\u2010Pereira","sequence":"additional","affiliation":[{"name":"Universidade Federal do Rio de Janeiro Instituto de Microbiologia Paulo G\u00f3es  Rio de Janeiro RJ Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. 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