{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T09:03:12Z","timestamp":1762506192603,"version":"build-2065373602"},"reference-count":38,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2013,10,4]],"date-time":"2013-10-04T00:00:00Z","timestamp":1380844800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["resjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Insect Molecular Biology"],"published-print":{"date-parts":[[2013,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Mosquitoes inject saliva into a vertebrate host during blood feeding. The analysis of mosquito saliva in host skin is important for the elucidation of the inflammatory responses to mosquito bites, the development of antithrombotic drugs, and the transmission\u2010blocking of vector\u2010borne diseases. We produced transgenic\n                    <jats:italic>\n                      <jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>\n                      nopheles stephensi\n                    <\/jats:italic>\n                    mosquitoes expressing the secretory luciferase protein (\n                    <jats:styled-content style=\"fixed-case\">M<\/jats:styled-content>\n                    et\n                    <jats:styled-content style=\"fixed-case\">L<\/jats:styled-content>\n                    uc) fused to a saliva protein (\n                    <jats:styled-content style=\"fixed-case\">AAPP<\/jats:styled-content>\n                    ) in the salivary glands. The transgene product (\n                    <jats:styled-content style=\"fixed-case\">AAPP<\/jats:styled-content>\n                    \u2010\n                    <jats:styled-content style=\"fixed-case\">M<\/jats:styled-content>\n                    et\n                    <jats:styled-content style=\"fixed-case\">L<\/jats:styled-content>\n                    uc) of transgenic mosquitoes exhibited both luciferase activity as a\n                    <jats:styled-content style=\"fixed-case\">M<\/jats:styled-content>\n                    et\n                    <jats:styled-content style=\"fixed-case\">L<\/jats:styled-content>\n                    uc and binding activity to collagen as an\n                    <jats:styled-content style=\"fixed-case\">AAPP<\/jats:styled-content>\n                    . The detection of luminescence in the skin of mice bitten by\n                    <jats:styled-content style=\"fixed-case\">transgenic<\/jats:styled-content>\n                    mosquitoes showed that\n                    <jats:styled-content style=\"fixed-case\">AAPP<\/jats:styled-content>\n                    \u2010\n                    <jats:styled-content style=\"fixed-case\">M<\/jats:styled-content>\n                    et\n                    <jats:styled-content style=\"fixed-case\">L<\/jats:styled-content>\n                    uc was injected into the skin as a component of saliva via blood feeding.\n                    <jats:styled-content style=\"fixed-case\">AAPP<\/jats:styled-content>\n                    \u2010\n                    <jats:styled-content style=\"fixed-case\">M<\/jats:styled-content>\n                    et\n                    <jats:styled-content style=\"fixed-case\">L<\/jats:styled-content>\n                    uc remained at the mosquito bite site in host skin with luciferase activity for at least 4\u2009h after blood feeding.\n                    <jats:styled-content style=\"fixed-case\">AAPP<\/jats:styled-content>\n                    was also suspected of remaining at the site of injury caused by the mosquito bite and blocking platelet aggregation by binding to collagen. These results demonstrated the establishment of visualization and time\u2010lapse analysis of mosquito saliva in living vertebrate host skin. This technique may facilitate the analysis of mosquito saliva after its injection into host skin, and the development of new drugs and disease control strategies.\n                  <\/jats:p>","DOI":"10.1111\/imb.12055","type":"journal-article","created":{"date-parts":[[2013,10,11]],"date-time":"2013-10-11T22:05:29Z","timestamp":1381529129000},"page":"685-693","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Visualization and live imaging analysis of a mosquito saliva protein in host animal skin using a transgenic mosquito with a secreted luciferase reporter system"],"prefix":"10.1111","volume":"22","author":[{"given":"D. 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