{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T21:46:30Z","timestamp":1791236790085,"version":"4.1.0"},"reference-count":57,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2013,12,3]],"date-time":"2013-12-03T00:00:00Z","timestamp":1386028800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["resjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Insect Molecular Biology"],"published-print":{"date-parts":[[2014,4]]},"abstract":"Abstract<\/jats:title>\n \n The mosquito\n \n A<\/jats:styled-content>\n edes aegypti\n <\/jats:italic>\n is the main vector of\n D<\/jats:styled-content>\n engue and\n Y<\/jats:styled-content>\n ellow\n F<\/jats:styled-content>\n ever flaviviruses. The organophosphate insecticide temephos is a larvicide that is used globally to control\n \n A<\/jats:styled-content>\n e. aegypti\n <\/jats:italic>\n populations; many of which have in turn evolved resistance. Target site alteration in the acetylcholine esterase of this species has not being identified. Instead, we tracked changes in transcription of metabolic detoxification genes using the\n \n A<\/jats:styled-content>\n e. aegypti \u2018\n D<\/jats:styled-content>\n etox\n C<\/jats:styled-content>\n hip\n <\/jats:italic>\n \u2019 microarray during five generations of temephos selection. We selected for temephos resistance in three replicates in each of six collections, five from\n M<\/jats:styled-content>\n exico, and one from\n P<\/jats:styled-content>\n eru. The response to selection was tracked in terms of lethal concentrations. Uniform upregulation was seen in the epsilon class glutathione\u2010\n S<\/jats:styled-content>\n \u2010transferase (\n eGST<\/jats:styled-content>\n ) genes in strains from\n M<\/jats:styled-content>\n exico prior to laboratory selection, while\n eGSTs<\/jats:styled-content>\n in the\n I<\/jats:styled-content>\n quitos\n P<\/jats:styled-content>\n eru strain became upregulated after five generations of temephos selection. While expression of many carboxyl\/cholinesterase esterase (\n CCE<\/jats:styled-content>\n ) genes increased with selection, no single esterase was consistently upregulated and this same pattern was noted in the cytochrome\n \n P\n 450<\/jats:sub>\n <\/jats:styled-content>\n monooxygenase (\n CYP<\/jats:styled-content>\n ) genes and in other genes involved in reduction or oxidation of xenobiotics. Bioassays using glutathione\u2010\n S<\/jats:styled-content>\n \u2010transferase (\n GST)<\/jats:styled-content>\n ,\n CCE<\/jats:styled-content>\n and\n CYP<\/jats:styled-content>\n inhibitors suggest that various\n CCEs<\/jats:styled-content>\n instead of\n GSTs<\/jats:styled-content>\n are the main metabolic mechanism conferring resistance to temephos. We show that temephos\u2010selected strains show no cross resistance to permethrin and that genes associated with temephos selection are largely independent of those selected with permethrin in a previous study.\n <\/jats:p>","DOI":"10.1111\/imb.12073","type":"journal-article","created":{"date-parts":[[2013,12,3]],"date-time":"2013-12-03T04:08:04Z","timestamp":1386043684000},"page":"199-215","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":53,"title":["Differential transcription profiles in\n \n A<\/scp>\n edes aegypti\n <\/i>\n detoxification genes after temephos selection"],"prefix":"10.1111","volume":"23","author":[{"given":"K.","family":"Saavedra\u2010Rodriguez","sequence":"first","affiliation":[{"name":"Department of Microbiology Colorado State University Fort Collins CO USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Strode","sequence":"additional","affiliation":[{"name":"Vector Group Pembroke Place Liverpool School of Tropical Medicine Liverpool UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. E.","family":"Flores","sequence":"additional","affiliation":[{"name":"Laboratorio de Entomolog\u00eda M\u00e9dica Facultad de Ciencias Biol\u00f3gicas Universidad Aut\u00f3noma de Nuevo Le\u00f3n San Nicolas de los Garza Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Garcia\u2010Luna","sequence":"additional","affiliation":[{"name":"Department of Microbiology Colorado State University Fort Collins CO USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Reyes\u2010Solis","sequence":"additional","affiliation":[{"name":"Department of Microbiology Colorado State University Fort Collins CO USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Ranson","sequence":"additional","affiliation":[{"name":"Vector Group Pembroke Place Liverpool School of Tropical Medicine Liverpool UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Hemingway","sequence":"additional","affiliation":[{"name":"Vector Group Pembroke Place Liverpool School of Tropical Medicine Liverpool UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"IV","given":"W. C.","family":"Black","sequence":"additional","affiliation":[{"name":"Department of Microbiology Colorado State University Fort Collins CO USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,12,3]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1590\/S1519-566X2007000200019","article-title":"Resistance of Aedes aegypti (L.) (Diptera : Culicidae) populations to organophosphates ternephos in the Paraiba State, Brazil","volume":"36","author":"Beserra E.B.","year":"2007","journal-title":"Neotrop Entomol"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2915.2011.00959.x"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1897\/05-267R2.1"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0074-02762004000200015"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/jmedent\/42.5.830"},{"key":"e_1_2_6_7_1","first-page":"133","article-title":"Biological and nonbiological modifications of organophosphorus compounds","volume":"44","author":"Dauterman W.C.","year":"1971","journal-title":"Bull World Health Organ"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.2987\/8756-971X(2006)22[672:MOIRIF]2.0.CO;2"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cimid.2004.03.013"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2010.09-0623"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2915.1998.00082.x"},{"key":"e_1_2_6_12_1","first-page":"155","article-title":"Comparative metabolism and selectivity of organophosphate and carbamate insecticides","volume":"44","author":"Hollingworth R.M.","year":"1971","journal-title":"Bull World Health Organ"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0965-1748(98)00049-6"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1603\/0046-225X(2007)36[506:TOTRIA]2.0.CO;2"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0965-1748(00)00123-5"},{"key":"e_1_2_6_16_1","first-page":"229","article-title":"Assessing the insecticide resistance of an Aedes aegypti strain in El Salvador","volume":"26","author":"Lazcano J.A.B.","year":"2009","journal-title":"Rev Panam Salud Publica"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0037-86822006000300006"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1186\/1756-3305-4-5"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2003.68.329"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0074-02762010000100019"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.2987\/11-6192.1"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2007.05.018"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2010.12.005"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0074-02762003000500020"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-10-494"},{"key":"e_1_2_6_26_1","first-page":"315","article-title":"Characterization of resistance to organophosphate, carbamate, and pyrethroid insecticides in field populations of Aedes aegypti from Venezuela","volume":"11","author":"Mazzarri M.B.","year":"1995","journal-title":"J Am Mosq Control Assoc"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2009.10.015"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2007.77.467"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.7.2574"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-8-36"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2011.01.007"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj20030169"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/29.9.e45"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1005658330108"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.2987\/10-6019.1"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2010.09.005"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2008.01.004"},{"key":"e_1_2_6_38_1","first-page":"59","article-title":"Resistance in some Caribbean populations of Aedes aegypti to several insecticides","volume":"11","author":"Rawlins S.C.","year":"1995","journal-title":"J Am Mosq Control Assoc"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1039\/9781847551375"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1603\/0022-2585-38.5.623"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1603\/0022-2585-39.6.882"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.2987\/5588.1"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2583.2007.00774.x"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.108.087924"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2583.2011.01113.x"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.2987\/5738.1"},{"key":"e_1_2_6_47_1","doi-asserted-by":"publisher","DOI":"10.1590\/S1415-47572004000200024"},{"key":"e_1_2_6_48_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0001595"},{"key":"e_1_2_6_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2007.09.007"},{"key":"e_1_2_6_50_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0039439"},{"key":"e_1_2_6_51_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1570-7458.1989.tb01247.x"},{"key":"e_1_2_6_52_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-009-1362-8"},{"key":"e_1_2_6_53_1","unstructured":"U.S. EPA. (2001)Reregistration Eligibility Decision (RED) for temephos.http:\/\/www.epa.gov\/oppsrrd1\/REDs\/temephos_red.htm. Accessed on 9 October 13."},{"key":"e_1_2_6_54_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj3250359"},{"key":"e_1_2_6_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0965-1748(01)00059-5"},{"key":"e_1_2_6_56_1","unstructured":"WHO(2009)Global insecticide use for vector\u2010borne disease control. 4th ed. \u2018WHO\/HTM\/NTD\/WHOPES\/GCDPP\/2009.6\u2019."},{"key":"e_1_2_6_57_1","first-page":"171","article-title":"Effects of synergists on the metabolism and toxicity of anticholinesterases","volume":"44","author":"Wilkinson C.F.","year":"1971","journal-title":"Bull World Health Organ"},{"key":"e_1_2_6_58_1","first-page":"315","article-title":"Selection and characterization of temephos resistance in a population of Aedes aegypti from Tortola, British Virgin Islands","volume":"15","author":"Wirth M.C.","year":"1999","journal-title":"J Am Mosq Control Assoc"}],"container-title":["Insect Molecular Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fimb.12073","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/resjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/imb.12073","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T13:22:25Z","timestamp":1762089745000},"score":1,"resource":{"primary":{"URL":"https:\/\/resjournals.onlinelibrary.wiley.com\/doi\/10.1111\/imb.12073"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,12,3]]},"references-count":57,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2014,4]]}},"alternative-id":["10.1111\/imb.12073"],"URL":"https:\/\/doi.org\/10.1111\/imb.12073","archive":["Portico"],"relation":{},"ISSN":["0962-1075","1365-2583"],"issn-type":[{"value":"0962-1075","type":"print"},{"value":"1365-2583","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,12,3]]},"assertion":[{"value":"2013-12-03","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}