{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T18:36:37Z","timestamp":1773254197221,"version":"3.50.1"},"reference-count":28,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2014,8,13]],"date-time":"2014-08-13T00:00:00Z","timestamp":1407888000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["resjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Insect Molecular Biology"],"published-print":{"date-parts":[[2014,12]]},"abstract":"Abstract<\/jats:title>\n \n Engineering sex\u2010specific sterility is critical for developing transgene\u2010based sterile insect technology. Targeted genome engineering achieved by customized zinc\u2010finger nuclease, transcription activator\u2010like effector nuclease (\n TALEN)<\/jats:styled-content>\n or clustered, regularly interspaced, short palindromic repeats\/Cas9 systems has been exploited extensively in a variety of model organisms; however, screening mutated individuals without a detectable phenotype is still challenging. In addition, genetically recessive mutations only detectable in homozygotes make the experiments time\u2010consuming. In the present study, we model a novel genetic system in the silkworm,\n \n B<\/jats:styled-content>\n ombyx mori\n <\/jats:italic>\n , that results in female\u2010specific sterility by combining transgenesis with\n TALEN<\/jats:styled-content>\n technologies. This system induces sex\u2010specific sterility at a high efficiency by targeting the female\u2010specific exon of the\n \n B<\/jats:styled-content>\n .\u2009mori doublesex (\n B<\/jats:styled-content>\n mdsx)\n <\/jats:italic>\n gene, which has sex\u2010specific splicing isoforms regulating somatic sexual development. Transgenic animals co\u2010expressing\n TALEN<\/jats:styled-content>\n left and right arms targeting the female\u2010specific\n \n B<\/jats:styled-content>\n mdsx\n <\/jats:italic>\n exon resulted in somatic mutations and female mutants lost fecundity because of lack of egg storage and abnormal external genitalia. The wild\u2010type sexual dimorphism of abdominal segment was not evident in mutant females. In contrast, there were no deleterious effects in mutant male moths. The current somatic\n TALEN<\/jats:styled-content>\n technologies provide a promising approach for future insect functional genetics, thus providing the basis for the development of attractive genetic alternatives for insect population management.\n <\/jats:p>","DOI":"10.1111\/imb.12125","type":"journal-article","created":{"date-parts":[[2014,8,13]],"date-time":"2014-08-13T21:45:03Z","timestamp":1407966303000},"page":"800-807","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":49,"title":["Transcription activator\u2010like effector nuclease (\n TALEN)<\/scp>\n \u2010mediated female\u2010specific sterility in the silkworm\n \n ,\n B<\/scp>\n ombyx mori\n <\/i>"],"prefix":"10.1111","volume":"23","author":[{"given":"J.","family":"Xu","sequence":"first","affiliation":[{"name":"Key Laboratory of Insect Developmental and Evolutionary Biology Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai China"},{"name":"University of Chinese Academy of Sciences Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y.","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Insect Developmental and Evolutionary Biology Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai China"},{"name":"University of Chinese Academy of Sciences Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Z.","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Insect Developmental and Evolutionary Biology Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai China"},{"name":"University of Chinese Academy of Sciences Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Ling","sequence":"additional","affiliation":[{"name":"Key Laboratory of Insect Developmental and Evolutionary Biology Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai China"},{"name":"University of Chinese Academy of Sciences Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.","family":"Zeng","sequence":"additional","affiliation":[{"name":"Key Laboratory of Insect Developmental and Evolutionary Biology Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai China"},{"name":"University of Chinese Academy of Sciences Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. 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