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We evaluated expression of available luciferase constructs in tick cell cultures derived from <jats:italic>Rhipicephalus<\/jats:italic> (<jats:italic>Boophilus<\/jats:italic>) <jats:italic>microplus<\/jats:italic>, an important vector of bovine babesiosis and anaplasmosis. Commercial promoters were evaluated for transcriptional activity driving luciferase expression in the tick cell lines. The human phosphoglycerate kinase (PGK) promoter resulted in detectable firefly luciferase activity within 2 days post\u2010transfection of the <jats:italic>R.\u00a0microplus<\/jats:italic> cell line BME26, with maximal activity at 5 days post\u2010transfection. Several other promoters were weaker or inactive in the tick cells, prompting identification and assessment of transcriptional activity of the homologous ribosomal protein L4 (rpL4, GenBank accession no.: KM516205) and elongation factor 1\u03b1 (EF\u20101\u03b1, GenBank accession no.: KM516204) promoters cloned from <jats:italic>R.\u00a0microplus<\/jats:italic>. Evaluation of luciferase expression driven by various promoters in tick cell culture resulted in selection of the <jats:italic>R.\u00a0microplus<\/jats:italic> rpL4 promoter and the human PGK promoter driving transcription of sequences encoding modified firefly and NanoLuc\u00ae luciferases for construction of a dual luciferase reporter system for use in tick cell culture<\/jats:bold>.<\/jats:p>","DOI":"10.1111\/imb.12172","type":"journal-article","created":{"date-parts":[[2015,4,18]],"date-time":"2015-04-18T02:39:14Z","timestamp":1429324754000},"page":"454-466","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Discovery, adaptation and transcriptional activity of two tick promoters: Construction of a dual luciferase reporter system for optimization of RNA interference in <i>rhipicephalus<\/i> (<i>boophilus<\/i>) <i>microplus<\/i> cell lines"],"prefix":"10.1111","volume":"24","author":[{"given":"A. 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