{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T13:07:55Z","timestamp":1762088875879,"version":"build-2065373602"},"reference-count":32,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2015,10,16]],"date-time":"2015-10-16T00:00:00Z","timestamp":1444953600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Japan Society for the Promotion of Science (JSPS) KAKENHI","award":["25252050","25292173","26460511","26660229"],"award-info":[{"award-number":["25252050","25292173","26460511","26660229"]}]}],"content-domain":{"domain":["resjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Insect Molecular Biology"],"published-print":{"date-parts":[[2016,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Ticks are obligate haematophagous arthropods that feed on vertebrate blood containing high levels of iron. The host\u2010derived iron reacts to oxygen in the tick's body, and then high levels of reactive oxygen species, including hydrogen peroxide (H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    ), may be generated. High levels of H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    cause oxidative stress to aerobic organisms. Therefore, antioxidant responses are necessary to control H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    . We focused on peroxiredoxins (Prxs), H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    \u2010scavenging enzymes. The sequence of\n                    <jats:italic>Haemaphysalis longicornis 2\u2010Cys Prx<\/jats:italic>\n                    (\n                    <jats:italic>HlPrx2<\/jats:italic>\n                    ) was identified from fat body cDNA libraries of this tick and recombinant HlPrx2 was then prepared using\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    . By comparison with the 2\u2010Cys Prxs of other organisms, we found two conserved cysteines in HlPrx2, Cys51 and Cys172. We examined the antioxidant activity of HlPrx2 and mutant proteins produced by a single base substitution, converting one or both of these cysteines into serines. The assays revealed that proteins containing Cys51 showed antioxidant activity when H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    was removed. Sodium dodecyl sulphate polyacrylamide gel electrophoresis and size\u2010exclusion chromatography demonstrated that only the wild\u2010type HlPrx2 formed homodimers and that all of the proteins that we made had a high molecular weight peak. These results indicate that both Cys51 and Cys172 are essential for the dimerization of HlPrx2, whereas only the Cys51 residue is necessary for antioxidant activity.\n                  <\/jats:p>","DOI":"10.1111\/imb.12193","type":"journal-article","created":{"date-parts":[[2015,10,16]],"date-time":"2015-10-16T02:06:52Z","timestamp":1444961212000},"page":"16-23","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Functional analysis of recombinant 2\u2010Cys peroxiredoxin from the hard tick\n                    <scp>\n                      <i>H<\/i>\n                    <\/scp>\n                    <i>aemaphysalis longicornis<\/i>"],"prefix":"10.1111","volume":"25","author":[{"given":"K.","family":"Kusakisako","sequence":"first","affiliation":[{"name":"Laboratory of Infectious Diseases, Joint Faculty of Veterinary Medicine Kagoshima University Korimoto Kagoshima Japan"},{"name":"Department of Pathological and Preventive Veterinary Science, The United Graduate School of Veterinary Science Yamaguchi University Yoshida Yamaguchi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"Masatani","sequence":"additional","affiliation":[{"name":"Joint Faculty of Veterinary Medicine Transboundary Animal Diseases Center, Kagoshima University Korimoto Kagoshima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"Miyata","sequence":"additional","affiliation":[{"name":"Laboratory of Food Chemistry, Department of Biochemistry and Biotechnology, Division of Molecular Function of Food, Faculty of Agriculture Kagoshima University Korimoto Kagoshima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. L.","family":"Galay","sequence":"additional","affiliation":[{"name":"Laboratory of Infectious Diseases, Joint Faculty of Veterinary Medicine Kagoshima University Korimoto Kagoshima Japan"},{"name":"Department of Veterinary Paraclinical Sciences, College of Veterinary Medicine University of the Philippines Los Ba\u00f1os Los Ba\u00f1os Laguna The Philippines"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Maeda","sequence":"additional","affiliation":[{"name":"Laboratory of Infectious Diseases, Joint Faculty of Veterinary Medicine Kagoshima University Korimoto Kagoshima Japan"},{"name":"Department of Pathological and Preventive Veterinary Science, The United Graduate School of Veterinary Science Yamaguchi University Yoshida Yamaguchi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. R.","family":"Talactac","sequence":"additional","affiliation":[{"name":"Laboratory of Infectious Diseases, Joint Faculty of Veterinary Medicine Kagoshima University Korimoto Kagoshima Japan"},{"name":"Department of Pathological and Preventive Veterinary Science, The United Graduate School of Veterinary Science Yamaguchi University Yoshida Yamaguchi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N.","family":"Tsuji","sequence":"additional","affiliation":[{"name":"Department of Parasitology Kitasato University School of Medicine Minami, Sagamihara Kanagawa Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Mochizuki","sequence":"additional","affiliation":[{"name":"Laboratory of Infectious Diseases, Joint Faculty of Veterinary Medicine Kagoshima University Korimoto Kagoshima Japan"},{"name":"Department of Pathological and Preventive Veterinary Science, The United Graduate School of Veterinary Science Yamaguchi University Yoshida Yamaguchi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Fujisaki","sequence":"additional","affiliation":[{"name":"Zen\u2010Noh Institute of Animal Health Ohja, Sakura Chiba Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"Tanaka","sequence":"additional","affiliation":[{"name":"Laboratory of Infectious Diseases, Joint Faculty of Veterinary Medicine Kagoshima University Korimoto Kagoshima Japan"},{"name":"Department of Pathological and Preventive Veterinary Science, The United Graduate School of Veterinary Science Yamaguchi University Yoshida Yamaguchi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,10,16]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinsphys.2010.05.019"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-8227(99)00037-6"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nsb0598-400"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molbiopara.2006.10.008"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444910007493"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0090661"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1051\/parasite\/1983585493"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-4658.2009.06985.x"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2010.3624"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1515\/BC.2002.040"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2009.2"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2004.12.008"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)68840-4"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/ert184"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.abb.2004.04.017"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2009.05.030"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2006-09-048728"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2013.07.036"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-015-4365-7"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.13-0274"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2012.10.543"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2011.03.017"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.05214-11"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M505362200"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1130481"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3024.2010.01201.x"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1995.1428"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0969-2126(00)00147-7"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M001943200"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2583.2001.00246.x"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20110380"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0968-0004(02)00003-8"}],"container-title":["Insect 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