{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T11:42:47Z","timestamp":1789386167514,"version":"build-2803163510"},"reference-count":43,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2015,11,23]],"date-time":"2015-11-23T00:00:00Z","timestamp":1448236800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31301693"],"award-info":[{"award-number":["31301693"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002701","name":"Ministry of Education","doi-asserted-by":"publisher","award":["B07030"],"award-info":[{"award-number":["B07030"]}],"id":[{"id":"10.13039\/501100002701","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["resjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Insect Molecular Biology"],"published-print":{"date-parts":[[2016,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The diamondback moth,\n                    <jats:italic>Plutella xylostella<\/jats:italic>\n                    , is a global pest of cruciferous vegetables. Abamectin resistance in a field population of\n                    <jats:italic>P. xylostella<\/jats:italic>\n                    was introgressed into the susceptible Roth strain. The resulting introgression strain Roth\u2010Abm showed 11\u2009000\u2010fold resistance to abamectin compared with Roth. An A309V substitution at the N\u2010terminus of the third transmembrane helix (M3) of the glutamate\u2010gated chloride channel of\n                    <jats:italic>P. xylostella<\/jats:italic>\n                    (\n                    <jats:italic>PxGluCl<\/jats:italic>\n                    ) was identified in Roth\u2010Abm. The frequency of the V309 allele of\n                    <jats:italic>PxGluCl<\/jats:italic>\n                    was 94.7% in Roth\u2010Abm, whereas no such allele was detected in Roth. A subpopulation of Roth\u2010Abm was kept without abamectin selection for 20 generations to produce a revertant strain, Roth\u2010Abm\u2010D. Abamectin resistance in Roth\u2010Abm\u2010D declined to 1150\u2010fold compared with Roth, with the V309 allele frequency decreased to 9.6%. After treatment of the Roth\u2010Abm\u2010D strain with 80 mg\/l abamectin the V309 allele frequency in the survivors increased to 55%. This demonstrates that the A309V mutation in\n                    <jats:italic>PxGluCl<\/jats:italic>\n                    is strongly associated with a 10\u2010fold increase in abamectin resistance in Roth\u2010Abm relative to Roth\u2010Abm\u2010D. Homology modelling and automated ligand docking results suggest that the A309V substitution allosterically modifies the abamectin\u2010binding site, as opposed to directly eliminating a key binding contact. Other resistance mechanisms to abamectin in Roth\u2010Abm are discussed besides the A309V mutation of\n                    <jats:italic>PxGluCl<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1111\/imb.12204","type":"journal-article","created":{"date-parts":[[2015,11,23]],"date-time":"2015-11-23T02:31:02Z","timestamp":1448245862000},"page":"116-125","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":82,"title":["A point mutation in the glutamate\u2010gated chloride channel of\n                    <scp>\n                      <i>P<\/i>\n                    <\/scp>\n                    <i>lutella xylostella<\/i>\n                    is associated with resistance to abamectin"],"prefix":"10.1111","volume":"25","author":[{"given":"X.","family":"Wang","sequence":"first","affiliation":[{"name":"College of Plant Protection Nanjing Agricultural University Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Plant Protection Nanjing Agricultural University Nanjing China"},{"name":"Institute of Plant and Environment Protection Beijing Academy of Agriculture and Forestry Sciences Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Plant Protection Nanjing Agricultural University Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Wu","sequence":"additional","affiliation":[{"name":"College of Plant Protection Nanjing Agricultural University Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. O.","family":"O'Reilly","sequence":"additional","affiliation":[{"name":"School of Natural Sciences and Psychology Liverpool John Moores University Liverpool UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"Wu","sequence":"additional","affiliation":[{"name":"College of Plant Protection Nanjing Agricultural University Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,11,23]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature13669"},{"key":"e_1_2_6_3_1","unstructured":"APRD. (2015)Arthropod pesticide resistance database.http:\/\/www.pesticideresistance.com\/. 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