{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T21:34:33Z","timestamp":1770413673346,"version":"3.49.0"},"reference-count":23,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2016,5,2]],"date-time":"2016-05-02T00:00:00Z","timestamp":1462147200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["resjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Insect Molecular Biology"],"published-print":{"date-parts":[[2016,8]]},"abstract":"Abstract<\/jats:title>\n \n The sterile insect technique (SIT) involves the mass release of sterile males to suppress insect pest populations. SIT has been improved for larval pests by the development of strains for female\u2010specific tetracycline\u2010suppressible (Tet\u2010off) embryonic lethal systems for male\u2010only populations. Here we describe the extension of this approach to the Mexican fruit fly,\n Anastrepha ludens<\/jats:italic>\n , using a Tet\u2010off driver construct with the\n Tet\u2010transactivator<\/jats:italic>\n (\n tTA<\/jats:italic>\n ) under embryo\u2010specific\n \n Anastrepha suspensa serendipity\n \u03b1<\/jats:bold>\n <\/jats:italic>\n (\n \n As\u2010sry\u2010\n \u03b1<\/jats:bold>\n <\/jats:italic>\n ) promoter regulation. In the absence of tetracycline, tTA acts upon a Tet\u2010response element linked to the pro\u2010apoptotic cell death gene lethal effector,\n head involuation defective<\/jats:italic>\n (\n hid<\/jats:italic>\n ), from\n A. ludens<\/jats:italic>\n (\n \n Alhid\n Ala2<\/jats:sup>\n <\/jats:italic>\n ) that contains a sex\u2010specific intron splicing cassette, resulting in female\u2010specific expression of the lethal effector. Parental adults double\u2010homozygous for the driver\/effector vectors were expected to yield male\u2010only progeny when reared on Tet\u2010free diet, but a complete lack of oviposited eggs resulted for each of the three strains tested. Ovary dissection revealed nonvitellogenic oocytes in\u00a0all strains that was reversible by feeding females tetracycline for 5 days after eclosion, resulting in male\u2010only adults in one strain. Presumably the\n \n sry\u2010\n \u03b1<\/jats:bold>\n <\/jats:italic>\n promoter exhibits prezygotic maternal expression as well as zygotic embryonic expression in\n A. ludens<\/jats:italic>\n , resulting in a Tet\u2010off sterility effect in addition to female\u2010specific lethality.\n <\/jats:p>","DOI":"10.1111\/imb.12238","type":"journal-article","created":{"date-parts":[[2016,5,2]],"date-time":"2016-05-02T11:24:57Z","timestamp":1462188297000},"page":"500-508","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":61,"title":["Tetracycline\u2010suppressible female lethality and sterility in the Mexican fruit fly,\n Anastrepha ludens<\/i>"],"prefix":"10.1111","volume":"25","author":[{"given":"M. F.","family":"Schetelig","sequence":"first","affiliation":[{"name":"Justus\u2010Liebig\u2010University Giessen, Institute for Insect Biotechnology Giessen Germany"},{"name":"USDA\/ARS, Center for Medical, Agricultural and Veterinary Entomology Gainesville FL USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Targovska","sequence":"additional","affiliation":[{"name":"Insect Pest Control Laboratory, Joint FAO\/IAEA Programme of Nuclear Techniques in Food and Agriculture Vienna Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. S.","family":"Meza","sequence":"additional","affiliation":[{"name":"Programa Moscafrut, SAGARPA\u2010IICA Metapa de Dominguez Chiapas Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Bourtzis","sequence":"additional","affiliation":[{"name":"Insect Pest Control Laboratory, Joint FAO\/IAEA Programme of Nuclear Techniques in Food and Agriculture Vienna Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. M.","family":"Handler","sequence":"additional","affiliation":[{"name":"USDA\/ARS, Center for Medical, Agricultural and Veterinary Entomology Gainesville FL USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,5,2]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.17.3389"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1471-4922(03)00144-2"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/1-4020-4051-2_25"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1283"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.12.5547"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2583.1999.00139.x"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0965-1748(00)00119-3"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1201\/9781420039399.sec1"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt769"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004380050839"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/jee\/48.4.459"},{"key":"e_1_2_6_13_1","first-page":"303","article-title":"Sterile insect technique for suppressing and eradicating insect population: 55 years and counting","volume":"15","author":"Krafsur E.S.","year":"1998","journal-title":"J Agric Entomol"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.2014.1372"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2012.10.010"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.129.15.3715"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(89)90272-8"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/30.9.e36"},{"key":"e_1_2_6_20_1","unstructured":"Salcedo\u2010Baca Diznarda(2010)Evaluaci\u00f3n econ\u00f3mica de la campa\u00f1a nacional contra las moscas de la fruta en los estados de Baja California Guerrero Nuevo Le\u00f3n Sinaloa Sonora y Tamaulipas (1994\u20102008)\/Salcedo\u2010Baca Diznardaet al.M\u00e9xico: IICA 2010."},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1203352109"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2012.07.007"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1186\/1741-7007-7-4"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.4.6.922"}],"container-title":["Insect Molecular Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fimb.12238","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/resjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/imb.12238","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T13:09:22Z","timestamp":1762088962000},"score":1,"resource":{"primary":{"URL":"https:\/\/resjournals.onlinelibrary.wiley.com\/doi\/10.1111\/imb.12238"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,5,2]]},"references-count":23,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2016,8]]}},"alternative-id":["10.1111\/imb.12238"],"URL":"https:\/\/doi.org\/10.1111\/imb.12238","archive":["Portico"],"relation":{},"ISSN":["0962-1075","1365-2583"],"issn-type":[{"value":"0962-1075","type":"print"},{"value":"1365-2583","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,5,2]]},"assertion":[{"value":"2016-05-02","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}