{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T08:20:41Z","timestamp":1762071641216,"version":"build-2065373602"},"reference-count":70,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2016,10,24]],"date-time":"2016-10-24T00:00:00Z","timestamp":1477267200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo (FAPESP)","award":["2012\/11654-9"],"award-info":[{"award-number":["2012\/11654-9"]}]},{"name":"FAPESP fellowship","award":["2012\/06272-0"],"award-info":[{"award-number":["2012\/06272-0"]}]}],"content-domain":{"domain":["resjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Insect Molecular Biology"],"published-print":{"date-parts":[[2017,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    MicroRNAs (miRNAs) are small noncoding RNAs that modulate gene expression through post\u2010transcriptional regulation. Here, we report the identification and characterization of miRNAs in two closely related screwworm flies with different feeding habits:\n                    <jats:italic>Cochliomyia hominivorax<\/jats:italic>\n                    and\n                    <jats:italic>Cochliomyia macellaria<\/jats:italic>\n                    . The New World screwworm,\n                    <jats:italic>C<\/jats:italic>\n                    .\n                    <jats:italic>hominivorax<\/jats:italic>\n                    , is an obligatory parasite of warm\u2010blooded vertebrates, whereas the secondary screwworm,\n                    <jats:italic>C<\/jats:italic>\n                    .\n                    <jats:italic>macellaria<\/jats:italic>\n                    , is a free\u2010living organism that feeds on decaying organic matter. Here, the small RNA transcriptomes of adults and third\u2010instar larvae of both species were sequenced. A total of 110 evolutionarily conserved miRNAs were identified, and 10 putative precursor miRNAs (pre\u2010miRNAs) were predicted. The relative expression of six selected miRNAs was further investigated, including miRNAs that are related to reproduction and neural processes in other insects. Mature miRNAs were also characterized across an evolutionary time scale, suggesting that the majority of them have been conserved since the emergence of the Arthropoda [540 million years ago (Ma)], Hexapoda (488 Ma) and Brachycera (195 Ma) lineages. This study is the first report of miRNAs for screwworm flies. We also performed a comparative analysis with the hereby predicted miRNAs from the sheep blowfly,\n                    <jats:italic>Lucilia cuprina<\/jats:italic>\n                    . The results presented may advance our understanding of parasitic habits within Calliphoridae and assist further functional studies in blowflies.\n                  <\/jats:p>","DOI":"10.1111\/imb.12270","type":"journal-article","created":{"date-parts":[[2016,10,24]],"date-time":"2016-10-24T11:27:20Z","timestamp":1477308440000},"page":"46-57","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Identification and characterization of microRNAs in the screwworm flies\n                    <i>Cochliomyia hominivorax<\/i>\n                    and\n                    <i>Cochliomyia macellaria<\/i>\n                    (Diptera: Calliphoridae)"],"prefix":"10.1111","volume":"26","author":[{"given":"D. F.","family":"Paulo","sequence":"first","affiliation":[{"name":"Centre for Molecular Biology and Genetic Engineering, Department of Genetics, Evolution and Bioagents Institute of Biology, University of Campinas Campinas SP Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. M. L.","family":"Azeredo\u2010Espin","sequence":"additional","affiliation":[{"name":"Centre for Molecular Biology and Genetic Engineering, Department of Genetics, Evolution and Bioagents Institute of Biology, University of Campinas Campinas SP Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L. E. C.","family":"Canesin","sequence":"additional","affiliation":[{"name":"Centre for Molecular Biology and Genetic Engineering, Department of Genetics, Evolution and Bioagents Institute of Biology, University of Campinas Campinas SP Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Vicentini","sequence":"additional","affiliation":[{"name":"Centre for Molecular Biology and Genetic Engineering, Department of Genetics, Evolution and Bioagents Institute of Biology, University of Campinas Campinas SP Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. C. M.","family":"Junqueira","sequence":"additional","affiliation":[{"name":"Singapore Centre for Environmental Life Sciences Engineering Nanyang Technological University  Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,10,24]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(05)80360-2"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms8344"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2012.10.005"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-12-328"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-384747-8.10002-9"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.0030085"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1016230107"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-015-1835-3"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1103472108"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2009-10-7-r78"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dci.2011.12.008"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0019350"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6941.2006.00060.x"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.4161\/rna.25481"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0084747"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg2290"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.114.167320"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkr688"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1601-183X.2012.00782.x"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1590\/S1984-29612014042"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0101-81751982000400001"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-308X(08)60073-1"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2014.10.007"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1051\/apido\/2010032"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.185579.114"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/imb.12152"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/imb.12111"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinsphys.2014.08.003"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2009.06.008"},{"key":"e_1_2_5_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-1-4832-3211-9.50009-7"},{"key":"e_1_2_5_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep21762"},{"key":"e_1_2_5_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2007.09.020"},{"key":"e_1_2_5_34_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkt1181"},{"key":"e_1_2_5_35_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1064921"},{"key":"e_1_2_5_36_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2009-10-3-r25"},{"key":"e_1_2_5_37_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-10-581"},{"key":"e_1_2_5_38_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.4004-12.2013"},{"key":"e_1_2_5_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2583.2012.01135.x"},{"key":"e_1_2_5_40_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-10-455"},{"key":"e_1_2_5_41_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1418522112"},{"key":"e_1_2_5_42_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msn040"},{"key":"e_1_2_5_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2012.10.009"},{"key":"e_1_2_5_44_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1424408112"},{"key":"e_1_2_5_45_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1004096"},{"key":"e_1_2_5_46_1","doi-asserted-by":"publisher","DOI":"10.1093\/gbe\/evq053"},{"key":"e_1_2_5_47_1","doi-asserted-by":"publisher","DOI":"10.1038\/35040556"},{"key":"e_1_2_5_48_1","doi-asserted-by":"publisher","DOI":"10.1002\/bies.200900033"},{"key":"e_1_2_5_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2013.08.003"},{"key":"e_1_2_5_50_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.6597907"},{"key":"e_1_2_5_51_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkv555"},{"key":"e_1_2_5_52_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1258040"},{"key":"e_1_2_5_53_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2008.73"},{"key":"e_1_2_5_54_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1006172107"},{"key":"e_1_2_5_55_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-014-0466-3"},{"key":"e_1_2_5_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2007.11.007"},{"key":"e_1_2_5_57_1","doi-asserted-by":"publisher","DOI":"10.1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