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The aim of this study was to determine the importance of P\u2010gp for susceptibility to insecticides in the beet armyworm (\n                    <jats:italic>Spodoptera exigua<\/jats:italic>\n                    ) using clustered regularly interspaced short palindromic repeats\/CRISPR\u2010associated 9 (CRISPR\/Cas9) gene\u2010editing technology. We cloned an open reading frame (ORF) encoding the\n                    <jats:italic>S.\u00a0exigua<\/jats:italic>\n                    P\u2010gp protein (SeP\u2010gp) predicted to display structural characteristics common to P\u2010gp and other insect ABCB1 transporters. A knockout line with a frame shift deletion of four nucleotides in the SeP\u2010gp ORF was established using the CRISPR\/Cas9 gene\u2010editing system to test its potential role in determining susceptibility to chemical insecticides or insecticidal proteins from the bacterium\n                    <jats:italic>Bacillus thuringiensis<\/jats:italic>\n                    (Bt). Results from comparative bioassays demonstrate that knockout of\n                    <jats:italic>SeP\u2010gp<\/jats:italic>\n                    significantly increases susceptibility of\n                    <jats:italic>S.\u00a0exigua<\/jats:italic>\n                    by around threefold to abamectin and emamectin benzoate (EB), but not to spinosad, chlorfenapyr, beta\u2010cypermethrin, carbosulfan indoxacarb, chlorpyrifos, phoxim, diafenthiuron, chlorfluazuron, chlorantraniliprole or two Bt toxins (Cry1Ca and Cry1Fa). Our data support an important role for SeP\u2010gp in susceptibility of\n                    <jats:italic>S.\u00a0exigua<\/jats:italic>\n                    to abamectin and EB and imply that overexpression of SeP\u2010gp may contribute to abamectin and EB resistance in\n                    <jats:italic>S.\u00a0exigua<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1111\/imb.12338","type":"journal-article","created":{"date-parts":[[2017,7,28]],"date-time":"2017-07-28T11:26:38Z","timestamp":1501241198000},"page":"36-45","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":90,"title":["Knockout of a P\u2010glycoprotein gene increases susceptibility to abamectin and emamectin benzoate in\n                    <i>Spodoptera exigua<\/i>"],"prefix":"10.1111","volume":"27","author":[{"given":"Y.\u2010Y.","family":"Zuo","sequence":"first","affiliation":[{"name":"College of Plant Protection Nanjing Agricultural 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