{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,9]],"date-time":"2026-04-09T06:04:11Z","timestamp":1775714651389,"version":"3.50.1"},"reference-count":62,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2017,12,13]],"date-time":"2017-12-13T00:00:00Z","timestamp":1513123200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100007541","name":"Agro-Industry R and D Special Fund of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007541","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["resjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Insect Molecular Biology"],"published-print":{"date-parts":[[2018,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    An obvious challenge faced by most terrestrial insects is maintaining water homeostasis in an arid environment. Current research suggests aquaporins may be evolved to meet the challenge. However, up to now, this suggestion has not been verified in any insect that feeds upon solid food with mandibulate mouthparts. In the present paper, nine putative aquaporin genes [\n                    <jats:italic>Tribolium castaneum big brain<\/jats:italic>\n                    ,\n                    <jats:italic>T.\u00a0castaneum Drosophila integral protein<\/jats:italic>\n                    (\n                    <jats:italic>TcDrip<\/jats:italic>\n                    ),\n                    <jats:italic>T.\u00a0castaneum Pyrocoelia rufa integral protein<\/jats:italic>\n                    (\n                    <jats:italic>TcPrip<\/jats:italic>\n                    ),\n                    <jats:italic>T.\u00a0castaneum aquaporin 12\u2010like<\/jats:italic>\n                    ,\n                    <jats:italic>T.\u00a0castaneum entomoglyceroporin 1<\/jats:italic>\n                    (\n                    <jats:italic>TcEglp1<\/jats:italic>\n                    ),\n                    <jats:italic>TcEglp2<\/jats:italic>\n                    ,\n                    <jats:italic>TcEglp3<\/jats:italic>\n                    ,\n                    <jats:italic>TcEglp4<\/jats:italic>\n                    and\n                    <jats:italic>TcEglp5<\/jats:italic>\n                    ] were identified in\n                    <jats:italic>T.\u00a0castaneum<\/jats:italic>\n                    . The transcripts of the nine genes were easily detectable in the foregut, midgut, hindgut\u2013Malpighian tubule complex, fat body and carcass (except gut and fat body). Amongst them\n                    <jats:italic>TcDrip<\/jats:italic>\n                    ,\n                    <jats:italic>TcPrip<\/jats:italic>\n                    ,\n                    <jats:italic>TcEglp1<\/jats:italic>\n                    ,\n                    <jats:italic>TcEglp3<\/jats:italic>\n                    and\n                    <jats:italic>TcEglp5<\/jats:italic>\n                    were highly transcribed in the hindgut\u2013Malpighian tubule complex;\n                    <jats:italic>TcEglp4<\/jats:italic>\n                    was abundantly expressed in both the fat body and hindgut\u2013Malpighian tubule complex. RNA interference (RNAi)\u2010mediated knockdown of\n                    <jats:italic>TcEglp3<\/jats:italic>\n                    caused a grey larval cuticle, in contrast to a smooth and bright cuticle in control larvae. Approximately 40% of the\n                    <jats:italic>TcEglp3<\/jats:italic>\n                    RNAi larvae had their hindguts protruding from the anus; their fresh wet faeces were attached to the hindgut. Another 20% of these treated larvae did not defaecate normally; wet brown faeces were adhered to the anal area. As a result, the larval growth was inhibited and about 60% larval lethality occurred. Silencing of\n                    <jats:italic>TcEglp4<\/jats:italic>\n                    or\n                    <jats:italic>TcDrip<\/jats:italic>\n                    exhibited similar but weaker defective phenotypes as those of the\n                    <jats:italic>TcEglp3<\/jats:italic>\n                    \u2010silenced larvae. Therefore, Eglp3, Eglp4 and Drip may contribute to the conductance of water in the alimentary canal and Malpighian tubules in\n                    <jats:italic>T.\u00a0castaneum<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1111\/imb.12367","type":"journal-article","created":{"date-parts":[[2017,12,13]],"date-time":"2017-12-13T08:22:42Z","timestamp":1513153362000},"page":"234-246","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["RNA interference\u2010mediated functional characterization of aquaporin genes in\n                    <i>Tribolium castaneum<\/i>"],"prefix":"10.1111","volume":"27","author":[{"given":"X.\u2010X.","family":"Yao","sequence":"first","affiliation":[{"name":"Key Laboratory of Integrated Crop Pest Management in Eastern China (Agricultural Ministry of China), College of Plant Protection Nanjing Agricultural University Nanjing 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