{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T22:09:24Z","timestamp":1791583764554,"version":"4.3.4"},"reference-count":46,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2019,2,13]],"date-time":"2019-02-13T00:00:00Z","timestamp":1550016000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31272047"],"award-info":[{"award-number":["31272047"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31360442"],"award-info":[{"award-number":["31360442"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["resjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Insect Molecular Biology"],"published-print":{"date-parts":[[2019,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Broad\u2010Complex (\n                    <jats:italic>BrC<\/jats:italic>\n                    ) is a downstream target of both 20\u2010hydroxyecdysone and juvenile hormone signalling.\n                    <jats:italic>BrC<\/jats:italic>\n                    regulates morphogenetic changes between nymphal instars in hemimetabolans, whereas it controls pupal commitment, pupal morphogenesis and inhibits adult differentiation in holometabolans. Among five\n                    <jats:italic>BrC<\/jats:italic>\n                    cDNAs (\n                    <jats:italic>Z1<\/jats:italic>\n                    \u2013\n                    <jats:italic>Z4<\/jats:italic>\n                    and\n                    <jats:italic>Z6<\/jats:italic>\n                    ) identified in the Colorado potato beetle, we found in this work that\n                    <jats:italic>Z1<\/jats:italic>\n                    ,\n                    <jats:italic>Z2<\/jats:italic>\n                    and\n                    <jats:italic>Z6<\/jats:italic>\n                    were mainly expressed at the last (fourth) instar and prepupal stages, whereas the levels of\n                    <jats:italic>Z3<\/jats:italic>\n                    and\n                    <jats:italic>Z4<\/jats:italic>\n                    increased during the penultimate (third) instar stage, peaked at the last instar larval phase and gradually decreased at the prepupal and pupal periods. When knocking down all\n                    <jats:italic>BrC<\/jats:italic>\n                    isoforms by RNA interference (RNAi) at the penultimate instar stage, around 20% of the resultant larvae remained as moribund beetles. These moribund\n                    <jats:italic>BrC<\/jats:italic>\n                    RNAi larvae were completely or partially wrapped in old cuticle. Likewise, a portion of larvae treated for a single double\u2010stranded RNA of\n                    <jats:italic>Z3<\/jats:italic>\n                    ,\n                    <jats:italic>Z4<\/jats:italic>\n                    or\n                    <jats:italic>Z6<\/jats:italic>\n                    displayed a degree of similar aberrancies, increasing in the order of isoforms Z6\u00a0&lt;\u00a0Z3\u00a0&lt;\u00a0Z4. When silencing all\n                    <jats:italic>BrC<\/jats:italic>\n                    isoforms at the last instar period, most of the RNAi larvae did not normally pupate or emerge as adults. Separately silencing each of the five zinc finger domains revealed that approximately 70% of the\n                    <jats:italic>Z1<\/jats:italic>\n                    RNAi larvae remained as prepupae, around 60% of the\n                    <jats:italic>Z6<\/jats:italic>\n                    RNAi specimens formed aberrant prepupae or pupae and about 60% of the\n                    <jats:italic>Z2<\/jats:italic>\n                    RNAi beetles became deformed pupae. After removal of the old exuviae, these deformed larvae in which either\n                    <jats:italic>Z1<\/jats:italic>\n                    ,\n                    <jats:italic>Z2<\/jats:italic>\n                    or\n                    <jats:italic>Z6<\/jats:italic>\n                    was depleted possessed adult prothorax and mesothorax, developing antenna, mouthparts and wing discs. Moreover, less than 50% of the resultant pupae finally emerged as adults when either of\n                    <jats:italic>Z1<\/jats:italic>\n                    ,\n                    <jats:italic>Z2<\/jats:italic>\n                    or\n                    <jats:italic>Z6<\/jats:italic>\n                    was knocked down. Therefore, our findings reveal, for the first time, that the two roles of\n                    <jats:italic>BrC<\/jats:italic>\n                    in insect groups (ie directing morphogenetic changes during juvenile development and regulating larval\u2013pupal\u2013adult metamorphosis) are played by different\n                    <jats:italic>BrC<\/jats:italic>\n                    isoforms in\n                    <jats:italic>Leptinotarsa decemlineata<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1111\/imb.12563","type":"journal-article","created":{"date-parts":[[2019,1,11]],"date-time":"2019-01-11T05:02:59Z","timestamp":1547182979000},"page":"420-430","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Isoform specific roles of Broad\u2010Complex in larval development in\n                    <i>Leptinotarsa decemlineata<\/i>"],"prefix":"10.1111","volume":"28","author":[{"given":"Q.\u2010Y.","family":"Xu","sequence":"first","affiliation":[{"name":"Education Ministry Key Laboratory of Integrated Management of Crop Diseases and Pests, College of Plant Protection Nanjing Agricultural University Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Q.\u2010W.","family":"Meng","sequence":"additional","affiliation":[{"name":"Education Ministry Key Laboratory of Integrated Management of Crop Diseases and Pests, College of Plant Protection Nanjing Agricultural University Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Deng","sequence":"additional","affiliation":[{"name":"Education Ministry Key Laboratory of Integrated Management of Crop Diseases and Pests, College of Plant Protection Nanjing Agricultural University Nanjing 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