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Recent progress on determining insect m6A function has been limited to the dipteran\n                    <jats:italic>Drosophila melanogaster<\/jats:italic>\n                    , in which m6A is involved in neuronal functions and sex determination. Methylation and function of m6A in other insects, however, remain unknown. Here, we investigated a transcriptome\u2010wide profile of m6A in the lepidopteran\n                    <jats:italic>Bombyx mori<\/jats:italic>\n                    and identified the methyltransferase subunits\n                    <jats:italic>B.\u00a0mori<\/jats:italic>\n                    methyltransferase\u2010like 3 (BmMETTL3) and BmMETTL14 in the m6A methylation pathway. Strikingly, loss of BmMETTL3 and BmMETTL14 in cultured\n                    <jats:italic>B<\/jats:italic>\n                    .\u00a0\n                    <jats:italic>mori<\/jats:italic>\n                    cells led to arrest of cell cycle progression and caused deficiency of chromosome alignment and segregation. Specifically, we identified 2853 m6A peaks representing transcripts of 2043 genes, and the target genes with m6A methylation were shown to be involved in gene expression and translation. It was interesting that we found that the highly expressed genes tended to be methylated by m6A, and comparative analysis of RNA m6A and DNA N6\u2010methyladenine (6mA) revealed two distinct regulatory mechanisms for gene expression. Overall, our work suggests RNA m6A and DNA 6mA play important roles in RNA and DNA epigenetic regulation in\n                    <jats:italic>B<\/jats:italic>\n                    .\u00a0\n                    <jats:italic>mori<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1111\/imb.12584","type":"journal-article","created":{"date-parts":[[2019,4,8]],"date-time":"2019-04-08T08:23:43Z","timestamp":1554711823000},"page":"703-715","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":46,"title":["Transcriptome\u2010wide analysis of N6\u2010methyladenosine uncovers its regulatory role in gene expression in the lepidopteran\n                    <i>Bombyx mori<\/i>"],"prefix":"10.1111","volume":"28","author":[{"given":"B.","family":"Li","sequence":"first","affiliation":[{"name":"Biological Science Research Center Southwest University Chongqing China"},{"name":"State Key Laboratory of Silkworm Genome Biology Southwest University Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"X.","family":"Wang","sequence":"additional","affiliation":[{"name":"Biological Science Research Center Southwest University Chongqing China"},{"name":"State Key Laboratory of Silkworm Genome Biology Southwest University Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8323-3245","authenticated-orcid":false,"given":"Z.","family":"Li","sequence":"additional","affiliation":[{"name":"Biological Science Research Center Southwest University Chongqing China"},{"name":"Chongqing Key Laboratory of Sericultural Science, Chongqing Engineering and Technology Research Center for Novel Silk Materials Southwest University Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Lu","sequence":"additional","affiliation":[{"name":"Biological Science Research Center Southwest University Chongqing 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