{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T05:11:46Z","timestamp":1784265106251,"version":"3.55.0"},"reference-count":49,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2021,1,7]],"date-time":"2021-01-07T00:00:00Z","timestamp":1609977600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100010038","name":"Earmarked Fund for China Agriculture Research System","doi-asserted-by":"publisher","award":["CARS\u201009\u2010P22"],"award-info":[{"award-number":["CARS\u201009\u2010P22"]}],"id":[{"id":"10.13039\/501100010038","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["resjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Insect Molecular Biology"],"published-print":{"date-parts":[[2021,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    In\n                    <jats:italic>Drosophila melanogaster<\/jats:italic>\n                    , ecdysis triggering hormone (ETH) is the key factor triggering ecdysis behaviour and promoting trachea clearance. However, whether ETH plays the dual roles in non\u2010dipteran insects is unknown. In this survey, we found that\n                    <jats:italic>Ldeth<\/jats:italic>\n                    mRNA levels were positively correlated with circulating 20\u2010hydroxyecdysone (20E) titers in\n                    <jats:italic>Leptinotarsa decemlineata<\/jats:italic>\n                    . Ingestion of an ecdysteroid agonist halofenozide or 20E stimulated the transcription of\n                    <jats:italic>Ldeth<\/jats:italic>\n                    , whereas RNA interference (RNAi) of ecdysteroidogenesis (\n                    <jats:italic>LdPTTH<\/jats:italic>\n                    or\n                    <jats:italic>LdSHD<\/jats:italic>\n                    ) or 20E signalling (\n                    <jats:italic>LdEcR<\/jats:italic>\n                    ,\n                    <jats:italic>LdUSP<\/jats:italic>\n                    or\n                    <jats:italic>LdFTZ\u2010F1<\/jats:italic>\n                    ) genes inhibited the expression, indicating ETH acts downstream of 20E. RNAi of\n                    <jats:italic>Ldeth<\/jats:italic>\n                    at the final instar stage impaired pupation. More than 80% of the\n                    <jats:italic>Ldeth<\/jats:italic>\n                    \u2010depleted beetles remained as prepupae, completely wrapped in the old larval cuticles. These prepupae became withered, dried and darkened gradually, and finally died in soil. The remaining\n                    <jats:italic>Ldeth<\/jats:italic>\n                    hypomorphs pupated and emerged as abnormal adults, bearing smaller and wrinkle elytrum and hindwing. Moreover, the tracheae in the\n                    <jats:italic>Ldeth<\/jats:italic>\n                    hypomorphs were full of liquid. We accordingly proposed that the failure of trachea clearance disenabled air\u2010swallowing after pupa\u2013adult ecdysis and impacted wing expansion. Our results suggest that ETH plays the dual roles, initiation of ecdysis and motivation of trachea clearance, in a coleopteran.\n                  <\/jats:p>","DOI":"10.1111\/imb.12691","type":"journal-article","created":{"date-parts":[[2020,12,27]],"date-time":"2020-12-27T12:45:25Z","timestamp":1609073125000},"page":"241-252","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Ecdysis triggering hormone is essential for larva\u2013pupa\u2013adult transformation in\n                    <scp>\n                      <i>Leptinotarsa decemlineata<\/i>\n                    <\/scp>"],"prefix":"10.1111","volume":"30","author":[{"given":"C.\u2010H.","family":"Shen","sequence":"first","affiliation":[{"name":"Education Ministry Key Laboratory of Integrated Management of Crop Diseases and Pests College of Plant Protection, Nanjing Agricultural University  Nanjing 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