{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T02:14:24Z","timestamp":1780452864460,"version":"3.54.1"},"reference-count":27,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2021,8,27]],"date-time":"2021-08-27T00:00:00Z","timestamp":1630022400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["resjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Insect Molecular Biology"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Body pigmentation is an important character of insects in adapting to biotic and abiotic environmental challenges. Additionally, based on the relative ease of screening, several genes involved in insect melanization have been used in classic genetic studies or as visual markers in constructing transgenic insects. Here, a homologue of the\n                    <jats:italic>Bombyx mori<\/jats:italic>\n                    melanization\u2010inhibiting gene\n                    <jats:italic>ebony<\/jats:italic>\n                    , associated with the conversion of dopamine to\n                    <jats:italic>N<\/jats:italic>\n                    \u2010\u03b2\u2010alanyl dopamine, was identified in a global pest,\n                    <jats:italic>Plutella xylostella<\/jats:italic>\n                    . The CRISPR\/Cas9 system was applied to generate multiple\n                    <jats:italic>Pxebony<\/jats:italic>\n                    knockout alleles which were crossed to produce a\n                    <jats:italic>Pxebony<\/jats:italic>\n                    knockout strain, showing darker pigmentation in larvae, pupae and adults, compared with wildtype. Interestingly, we observed that\n                    <jats:italic>Pxebony<\/jats:italic>\n                    heterozygotes displayed an intermediate darkened phenotype, indicating partial dominance between the knockout and wildtype alleles. The fitness costs of\n                    <jats:italic>Pxebony<\/jats:italic>\n                    deficiency were also assessed in the mutant strain, indicating that embryo hatchability and larval survival were significantly reduced, while the eclosion rate was not obviously affected. Our work provides a potential target for exploring CRISPR\u2010based genetics\u2010control systems in this economically important pest lepidopteran.\n                  <\/jats:p>","DOI":"10.1111\/imb.12730","type":"journal-article","created":{"date-parts":[[2021,8,20]],"date-time":"2021-08-20T04:51:17Z","timestamp":1629435077000},"page":"615-623","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["<scp>CRISPR<\/scp>\n                    \/Cas9\u2010based functional characterization of the pigmentation gene\n                    <i>ebony<\/i>\n                    in\n                    <i>Plutella xylostella<\/i>"],"prefix":"10.1111","volume":"30","author":[{"given":"X.","family":"Xu","sequence":"first","affiliation":[{"name":"State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops Institute of Applied Ecology, Fujian Agriculture and Forestry University  Fuzhou China"},{"name":"Joint International Research Laboratory of Ecological Pest Control, Ministry of Education Fujian Agriculture and Forestry University  Fuzhou China"},{"name":"Key Laboratory of Integrated Pest Management for Fujian\u2010Taiwan Crops Ministry of Agriculture  Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Harvey\u2010Samuel","sequence":"additional","affiliation":[{"name":"Arthropod Genetics Group The Pirbright Institute  Woking UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Yang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops Institute of Applied Ecology, Fujian Agriculture and Forestry University  Fuzhou China"},{"name":"Joint International Research Laboratory of Ecological Pest Control, Ministry of Education Fujian Agriculture and Forestry University  Fuzhou China"},{"name":"Key Laboratory of Integrated Pest Management for Fujian\u2010Taiwan Crops Ministry of Agriculture  Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"You","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops Institute of Applied Ecology, Fujian Agriculture and Forestry University  Fuzhou China"},{"name":"Joint International Research Laboratory of Ecological Pest Control, Ministry of Education Fujian Agriculture and Forestry University  Fuzhou China"},{"name":"Key Laboratory of Integrated Pest Management for Fujian\u2010Taiwan Crops Ministry of Agriculture  Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Alphey","sequence":"additional","affiliation":[{"name":"Joint International Research Laboratory of Ecological Pest Control, Ministry of Education Fujian Agriculture and Forestry University  Fuzhou China"},{"name":"Arthropod Genetics Group The Pirbright Institute  Woking 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