{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T04:51:51Z","timestamp":1785214311384,"version":"3.55.0"},"reference-count":33,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,8,30]],"date-time":"2022-08-30T00:00:00Z","timestamp":1661817600000},"content-version":"am","delay-in-days":356,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#am"},{"start":{"date-parts":[[2021,9,8]],"date-time":"2021-09-08T00:00:00Z","timestamp":1631059200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["IOS\u20101944214"],"award-info":[{"award-number":["IOS\u20101944214"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["355 R15AI139861"],"award-info":[{"award-number":["355 R15AI139861"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["resjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Insect Molecular Biology"],"published-print":{"date-parts":[[2022,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Understanding the development process of male and female mosquitoes provides important basic information for sterile insect release programmes and is important for improving other vector control strategies. However, little is known about the molecular mechanisms that distinguish male from female\u2010specific developmental processes in this species. We used IlluminaRNA\u2010seq to identify sex\u2010specific genes during pupal and adult stages. One hundred and forty\u2010seven genes were expressed only in pupal males, 56 genes were expressed in adult males and another 82 genes were commonly expressed in both male samples. In addition, 26 genes were expressed only in the pupal females, 163 genes were found in the adult females and only one gene was expressed in both female samples. A further quantitative real\u2010time PCR validation of selected genes from the RNA sequencing (RNA\u2010seq) analysis confirmed upregulation of those genes in a sex\u2010specific manner, including: fibrinogen and fibronectin, a zinc finger protein, phospholipase A(2) and a serine protein for female pupae; venom allergen 3, a perlecan, testis\u2010specific serine\/threonine\u2010protein kinase 1, testis\u2010specific serine\/threonine\u2010protein kinase 6 and cytochrome c\u20102 for male pupae; a salivary protein, D7 protein precursor, trypsin 7 precursor, D7 protein and nanos for female adults; and tetraspanin F139, cytosol aminopeptidase, testis\u2010specific serine\/threonine\u2010protein kinase 1, a testis\u2010specific serine\/threonine\u2010protein kinase 6 and a C\u2010type lectin for male adults. These findings provide insight into the development and physiology of\n                    <jats:italic>Culex<\/jats:italic>\n                    mosquitoes, which will help in the development of more effective control methods for these disease vectors.\n                  <\/jats:p>","DOI":"10.1111\/imb.12735","type":"journal-article","created":{"date-parts":[[2021,8,30]],"date-time":"2021-08-30T09:38:52Z","timestamp":1630316332000},"page":"24-32","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Transcriptome profiling reveals sex\u2010specific gene expressions in pupal and adult stages of the mosquito\n                    <i>Culex pipiens<\/i>"],"prefix":"10.1111","volume":"31","author":[{"given":"T.","family":"Martynova","sequence":"first","affiliation":[{"name":"Department of Biology Baylor University  Waco TX USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Kamanda","sequence":"additional","affiliation":[{"name":"Department of Biology Baylor University  Waco TX USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Sim","sequence":"additional","affiliation":[{"name":"Department of Biology Baylor University  Waco TX USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,9,8]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/v10070367"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu638"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/jme\/tjab024"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0002946"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu170"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/arch.21506"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2020.11.009"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/jisesa\/iey103"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-020-04078-2"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.RA118.001067"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.22653"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-015-0908-0"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1534\/g3.119.401006"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.onehlt.2017.06.001"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaa2850"},{"key":"e_1_2_7_17_1","unstructured":"Hannon G.J.(2010) FASTX\u2010toolkit. 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