{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:35:15Z","timestamp":1790814915015,"version":"4.1.0"},"reference-count":35,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2021,11,25]],"date-time":"2021-11-25T00:00:00Z","timestamp":1637798400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["DFG grant MO1714\/10\u20101"],"award-info":[{"award-number":["DFG grant MO1714\/10\u20101"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31730074\/31801749\/31672364\/31761133021"],"award-info":[{"award-number":["31730074\/31801749\/31672364\/31761133021"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["resjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Insect Molecular Biology"],"published-print":{"date-parts":[[2022,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Insects prevent uncontrolled penetration of water and xenobiotics by producing an impermeable cuticle. The major component of the cuticle is chitin that adopts a crystalline structure thereby contributing to cuticle stability. Our understanding of the contribution of chitin to the cuticle barrier function is limited. Here, we studied the role of the DOMON domain protein Knickkopf (LmKnk) that is involved in chitin organization and cuticle permeability in the migratory locust\n                    <jats:italic>Locusta migratoria<\/jats:italic>\n                    . We show that LmKnk localizes to the chitin layer in the newly produced cuticle. Injection of double\u2010stranded RNA targeting\n                    <jats:italic>LmKnk<\/jats:italic>\n                    (ds\n                    <jats:italic>LmKnk<\/jats:italic>\n                    ) in locust nymphs caused failure of moulting to the next stage. Histological experiments revealed that apolysis, i.e., the detachment of the old cuticle from the body surface, was normal; however, the newly synthesized cuticle was thinner than the cuticle of the control insects. Indeed, chitin content dropped after suppression of\n                    <jats:italic>LmKnk<\/jats:italic>\n                    expression. As seen by transmission electron microscopy, crystalline chitin organization was lost in ds\n                    <jats:italic>LmKnk<\/jats:italic>\n                    \u2010treated insects. In addition, the structure of pore canals, which are lipid transporting routes in the cuticle, was abnormal. Consistently, their content was reduced and, probably by consequence, lipid deposition on the cuticle was decreased after injection of ds\n                    <jats:italic>LmKnk<\/jats:italic>\n                    . Suppression of\n                    <jats:italic>LmKnk<\/jats:italic>\n                    transcript levels rendered\n                    <jats:italic>L. migratoria<\/jats:italic>\n                    more susceptible to each of four selected insecticides including malathion, chlorpyrifos, carbaryl and deltamethrin. Overall, our data show that\n                    <jats:italic>LmKnk<\/jats:italic>\n                    is needed for correct chitin amounts and organization, and their changes ultimately affect cuticular permeability in\n                    <jats:italic>L. migratoria<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1111\/imb.12745","type":"journal-article","created":{"date-parts":[[2021,11,5]],"date-time":"2021-11-05T07:59:15Z","timestamp":1636099155000},"page":"127-138","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["The\n                    <scp>DOMON<\/scp>\n                    domain protein\n                    <scp>LmKnk<\/scp>\n                    contributes to correct chitin content, pore canal formation and lipid deposition in the cuticle of\n                    <i>Locusta migratoria<\/i>\n                    during moulting"],"prefix":"10.1111","volume":"31","author":[{"given":"R. R.","family":"Yu","sequence":"first","affiliation":[{"name":"Institute of Applied Biology and College of Life Science Shanxi University  Taiyuan China"},{"name":"Department of Biology Taiyuan Normal University  Jinzhong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of Applied Biology and College of Life Science Shanxi University  Taiyuan China"},{"name":"College of Life Science Datong University  Datong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W. M.","family":"Liu","sequence":"additional","affiliation":[{"name":"Institute of Applied Biology and College of Life Science Shanxi University  Taiyuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"X. M.","family":"Zhao","sequence":"additional","affiliation":[{"name":"Institute of Applied Biology and College of Life Science Shanxi University  Taiyuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K. Y.","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Entomology Kansas State University  Manhattan Kansas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2854-9500","authenticated-orcid":false,"given":"B.","family":"Moussian","sequence":"additional","affiliation":[{"name":"Universit\u00e9 C\u00f4te d'Azur, CNRS, Inserm, Institute of Biology Valrose, Parc Valrose  Nice France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3400-0044","authenticated-orcid":false,"given":"J. Z.","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of Applied Biology and College of Life Science Shanxi University  Taiyuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,11,25]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1002682"},{"key":"e_1_2_9_3_1","first-page":"4864","article-title":"On a twisted fibrillar arrangement common to several biologic structures","volume":"261","author":"Bouligand Y.","year":"1965","journal-title":"Comptes Rendus de l'Acad\u00e9mie des Sciences"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1112288108"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1004537"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2015.02.013"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.0020239"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2010.01.011"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2017.08.003"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.10.4.589"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-7-11"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2010.03.003"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.02177"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-384747-8.10007-8"},{"key":"e_1_2_9_16_1","first-page":"315","article-title":"Circadian organization of chitin in some insect skeletons","volume":"106","author":"Neville A.C.","year":"1965","journal-title":"Quarterly Journal of Microscopical Science"},{"key":"e_1_2_9_17_1","unstructured":"Nicholas K.&Nicholas H.(1997) GeneDoc: a tool for editing and annotating multiple sequence alignments. 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