{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T20:17:11Z","timestamp":1789157831834,"version":"build-2803163510"},"reference-count":37,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2022,2,5]],"date-time":"2022-02-05T00:00:00Z","timestamp":1644019200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31672359"],"award-info":[{"award-number":["31672359"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31970657"],"award-info":[{"award-number":["31970657"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["resjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Insect Molecular Biology"],"published-print":{"date-parts":[[2022,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The troponin (Tn) complex, the sensor for Ca\n                    <jats:sup>2+<\/jats:sup>\n                    to regulate contraction of striated muscle, is composed of three subunits, that is, TnT, TnI and TnC. Different isoforms of TnI and TnC are expressed in the thorax dorsal longitudinal muscle (flight muscle) and the hind leg extensor tibiae muscle (jump muscle) of the migratory locust,\n                    <jats:italic>Locusta migratoria<\/jats:italic>\n                    . The major Tn complexes in the flight muscle and the jump muscle are Tn\u2010171 (TnT1\/TnI7\/TnC1) and Tn\u2010153 (TnT1\/TnI5\/TnC3), respectively. Here, we prepared a number of recombinant Tn complexes and the reconstituted thin filaments, and investigated their regulation on thin filament. Although both Tn\u2010171 and Tn\u2010153 regulate thin filament in a Ca\n                    <jats:sup>2+<\/jats:sup>\n                    \u2010dependent manner, the extent of Ca\n                    <jats:sup>2+<\/jats:sup>\n                    activation mediated by Tn\u2010171 was significantly lower than that by Tn\u2010153. We demonstrated that TnC1 and TnC3, rather than TnI5 and TnI7, are responsible for the different levels of the thin filament activation. Mutagenesis of TnC1 and TnC3 shows that the low level of TnC1\u2010mediated thin filament activation can be attributed to the noncanonical residue Leu60 in the EF\u2010hand\u2010II of TnC1. We therefore propose that, in addition to Ca\n                    <jats:sup>2+<\/jats:sup>\n                    , other regulatory mechanism(s) is required for the full activation of locust flight muscle.\n                  <\/jats:p>","DOI":"10.1111\/imb.12763","type":"journal-article","created":{"date-parts":[[2022,1,27]],"date-time":"2022-01-27T06:08:10Z","timestamp":1643263690000},"page":"346-355","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["<i>Locusta migratoria<\/i>\n                    flight muscle troponin partially activates thin filament in a calcium\u2010dependent manner"],"prefix":"10.1111","volume":"31","author":[{"given":"Hui\u2010Fang","family":"Liang","sequence":"first","affiliation":[{"name":"State Key Laboratory of Integrated Management of Insect Pests and Rodents Institute of Zoology, Chinese Academy of Sciences  Beijing China"},{"name":"University of Chinese Academy of Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8677-9833","authenticated-orcid":false,"given":"Xiang\u2010dong","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Integrated Management of Insect Pests and Rodents Institute of Zoology, Chinese Academy of Sciences  Beijing China"},{"name":"University of Chinese Academy of Sciences  Beijing 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