{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T10:24:36Z","timestamp":1778667876847,"version":"3.51.4"},"reference-count":82,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2022,4,18]],"date-time":"2022-04-18T00:00:00Z","timestamp":1650240000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["resjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Insect Molecular Biology"],"published-print":{"date-parts":[[2022,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Bradysia cellarum<\/jats:italic>\n                    (Diptera: Sciaridae) is a destructive vegetable insect pest infesting more than 30 species of host plants from seven families in Asia and Europe.\n                    <jats:italic>B. cellarum<\/jats:italic>\n                    causes grave problems in Chinese chive, which originated in China and is cultivated widely in East Asia. The\n                    <jats:italic>B. cellarum<\/jats:italic>\n                    infestation results in economic losses and subsequent severe food safety problems in farm productions, insecticide resistance and environmental pollution. The genomic and molecular information of\n                    <jats:italic>B. cellarum<\/jats:italic>\n                    to delineate the biological features, insecticide resistance, evolution remains poorly understood. Herein, we decode the whole genome of\n                    <jats:italic>B. cellarum<\/jats:italic>\n                    to delineate the underlying molecular mechanisms causing insecticide resistance. We constructed a highly reliable genome for\n                    <jats:italic>B. cellarum<\/jats:italic>\n                    using PacBio, Illumina and 10X Genomics sequencing platforms. The genome size of\n                    <jats:italic>B. cellarum<\/jats:italic>\n                    was 375.91\u2009Mb with a contig N50 of 1.57\u2009Mb. A total of 16,231 genes were identified, among which 93.8% were functionally annotated, and 42.06% were repeat sequences. According to phylogenetic analysis,\n                    <jats:italic>B. cellarum<\/jats:italic>\n                    diverged from the common ancestor of\n                    <jats:italic>Drosophila melanogaster<\/jats:italic>\n                    and\n                    <jats:italic>Musca domestica<\/jats:italic>\n                    ~139.3\u2013191.0 million years ago. Moreover, some important genes responsible for significant insecticide resistance, such as cytochrome P450s, ABC transporters and those involved in glutathione metabolism, were expanded in\n                    <jats:italic>B. cellarum<\/jats:italic>\n                    . We assembled a high\u2010quality\n                    <jats:italic>B. cellarum<\/jats:italic>\n                    genome to provide valuable insights into their life history strategies, insecticide resistance and biological behaviours. It also lays the foundation for exploring gene structure and functional evolution, as well as comparative genomics of\n                    <jats:italic>B. cellarum<\/jats:italic>\n                    and other model insect species.\n                  <\/jats:p>","DOI":"10.1111\/imb.12776","type":"journal-article","created":{"date-parts":[[2022,4,7]],"date-time":"2022-04-07T08:49:34Z","timestamp":1649321374000},"page":"508-518","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["De novo genome assembly of\n                    <i>Bradysia cellarum<\/i>\n                    (Diptera: Sciaridae), a notorious pest in traditional special vegetables in China"],"prefix":"10.1111","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2106-1833","authenticated-orcid":false,"given":"Mengyao","family":"Li","sequence":"first","affiliation":[{"name":"College of Plant Protection Hebei Agricultural University  Baoding China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaofan","family":"Yang","sequence":"additional","affiliation":[{"name":"Plant protection Institute Hebei Academy of Agricultural and Forestry Sciences  Baoding China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fan","family":"Fan","sequence":"additional","affiliation":[{"name":"College of Plant Protection Hebei Agricultural University  Baoding China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yafei","family":"Ge","sequence":"additional","affiliation":[{"name":"College of Plant Protection Hebei Agricultural University  Baoding China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dawei","family":"Hong","sequence":"additional","affiliation":[{"name":"College of Plant Science Tibet Agricultural and Animal Husbandry University  Nyingchi, Tibet China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhongyan","family":"Wang","sequence":"additional","affiliation":[{"name":"The Technical Education Centre of Nangong City  Xingtai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chenyan","family":"Lu","sequence":"additional","affiliation":[{"name":"College of Plant Science&amp;Technology Huazhong Agricultural University  Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suyi","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Plant Protection China Agricultural University  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guoshu","family":"Wei","sequence":"additional","affiliation":[{"name":"College of Plant Protection Hebei Agricultural University  Baoding 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