{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T11:31:34Z","timestamp":1762083094416,"version":"build-2065373602"},"reference-count":27,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2022,7,15]],"date-time":"2022-07-15T00:00:00Z","timestamp":1657843200000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2022,7,15]],"date-time":"2022-07-15T00:00:00Z","timestamp":1657843200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000152","name":"Division of Molecular and Cellular Biosciences","doi-asserted-by":"publisher","award":["1930237"],"award-info":[{"award-number":["1930237"]}],"id":[{"id":"10.13039\/100000152","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["resjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Insect Molecular Biology"],"published-print":{"date-parts":[[2022,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Chiffon is the sole\n                    <jats:italic>Drosophila<\/jats:italic>\n                    ortholog of Dbf4, the regulatory subunit for the cell\u2010cycle kinase Cdc7 that initiates DNA replication. In\n                    <jats:italic>Drosophila<\/jats:italic>\n                    , the\n                    <jats:italic>chiffon<\/jats:italic>\n                    gene encodes two polypeptides with independent activities. Chiffon\u2010A contains the conserved Dbf4 motifs and interacts with Cdc7 to form the Dbf4\u2010dependent Kinase (DDK) complex, which is essential for a specialized form of DNA replication. In contrast, Chiffon\u2010B binds the histone acetyltransferase Gcn5 to form the Chiffon histone acetyltransferase (CHAT) complex, which is necessary for histone H3 acetylation and viability. Previous studies have shown that the Chiffon\u2010B region is only present within insects. However, it was unclear how widely the interaction between Chiffon\u2010B and Gcn5 was conserved among insect species. To examine this, we performed yeast two\u2010hybrid assays using Chiffon\u2010B and Gcn5 from a variety of insect species and found that Chiffon\u2010B and Gcn5 interact in Diptera species such as Australian sheep blowfly and yellow fever mosquito. Protein domain analysis identified that Chiffon\u2010B has features of acidic transcriptional activators such as Gal4 or VP16. We propose that the CHAT complex plays a critical role in a biological process that is unique to Dipterans and could therefore be a potential target for pest control strategies.\n                  <\/jats:p>","DOI":"10.1111\/imb.12800","type":"journal-article","created":{"date-parts":[[2022,7,4]],"date-time":"2022-07-04T22:39:00Z","timestamp":1656974340000},"page":"734-746","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The interaction between the Dbf4 ortholog Chiffon and Gcn5 is conserved in Dipteran insect species"],"prefix":"10.1111","volume":"31","author":[{"given":"Smitha","family":"George","sequence":"first","affiliation":[{"name":"Department of Biochemistry Purdue University  West Lafayette Indiana USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hannah R.","family":"Blum","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Purdue University  West Lafayette Indiana USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eliana F.","family":"Torres\u2010Zelada","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Purdue University  West Lafayette Indiana USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Grace N.","family":"Estep","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Purdue University  West Lafayette Indiana USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Youssef A.","family":"Hegazy","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Purdue University  West Lafayette Indiana USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3766-6995","authenticated-orcid":false,"given":"Gina M.","family":"Speer","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Purdue University  West Lafayette Indiana USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5933-9952","authenticated-orcid":false,"given":"Vikki M.","family":"Weake","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Purdue University  West Lafayette Indiana USA"},{"name":"Purdue University Center for Cancer Research Purdue University  West Lafayette Indiana USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,7,15]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.12.4.480"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.25.18.8228-8238.2005"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ento.47.091201.145251"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.15.3.1220"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.26.3.871-882.2006"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1387\/ijdb.103194hh"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.23.9.3305-3319.2003"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.126.19.4281"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1525-142X.2005.05066.x"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.2000.3281"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.4161\/cc.24416"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.2000.4034"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.23.1.306-321.2003"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1210604"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a026047"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/335563a0"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.68068"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/129.4.1119"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/eru188"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.2005687"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkp885"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkz042"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M114.597948"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagrm.2020.194610"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.214072"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.259132"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp033"}],"container-title":["Insect 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