{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T07:18:21Z","timestamp":1773645501898,"version":"3.50.1"},"reference-count":18,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2022,8,17]],"date-time":"2022-08-17T00:00:00Z","timestamp":1660694400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002923","name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002923","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["resjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Insect Molecular Biology"],"published-print":{"date-parts":[[2022,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The choice of criteria to delimit a group or class is a subjective matter, even though the reasoning, the objectives and the criteria themselves should always be clearly stated. This paper is part of a discussion about the criteria used to identify seminal fluid proteins (SFPs) in\n                    <jats:italic>Drosophila<\/jats:italic>\n                    species. SFPs are proteins that are transferred to females during copulation together with sperm. The only way to ascertain that a protein is an SFP is to prove that it is produced in a male reproductive organ and is found in the female reproductive tract after insemination. Nevertheless, the required methodology is labour\u2010intensive and expensive, and therefore this kind of data is unlikely to be available for many species, precluding comparative and evolutionary studies on the subject. To conduct evolutionary analyses, in a previous study, we capitalized on the accumulated knowledge we have in the model species\n                    <jats:italic>D. melanogaster<\/jats:italic>\n                    to recommend a set of criteria for identifying candidate SFPs in other\n                    <jats:italic>Drosophila<\/jats:italic>\n                    species. Those criteria, based on transcriptomic evidence and in silico predictions from sequences, would allow a good balance between sensitivity (the inclusion of true SFPs) and specificity (the exclusion of false positives). In view of the criticism raised by another group, here we defend our criteria on one hand while accepting there is room for improvement on the other. The results are updated sets of criteria and SFPs that we believe can be useful in future evolutionary studies.\n                  <\/jats:p>","DOI":"10.1111\/imb.12806","type":"journal-article","created":{"date-parts":[[2022,8,5]],"date-time":"2022-08-05T01:45:00Z","timestamp":1659663900000},"page":"537-542","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["On how to identify a seminal fluid protein: A response to Wigby et al."],"prefix":"10.1111","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6496-3477","authenticated-orcid":false,"given":"Juan","family":"Hurtado","sequence":"first","affiliation":[{"name":"Departamento de Ecolog\u00eda, Gen\u00e9tica y Evoluci\u00f3n, Facultad de Ciencias Exactas y Naturales Universidad de Buenos Aires (UBA), CABA  Buenos Aires Argentina"},{"name":"Instituto de Ecolog\u00eda, Gen\u00e9tica y Evoluci\u00f3n de Buenos Aires Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), CABA  Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francisca Cunha","family":"Almeida","sequence":"additional","affiliation":[{"name":"Departamento de Ecolog\u00eda, Gen\u00e9tica y Evoluci\u00f3n, Facultad de Ciencias Exactas y Naturales Universidad de Buenos Aires (UBA), CABA  Buenos Aires Argentina"},{"name":"Instituto de Ecolog\u00eda, Gen\u00e9tica y Evoluci\u00f3n de Buenos Aires Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), CABA  Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvina Anah\u00ed","family":"Belliard","sequence":"additional","affiliation":[{"name":"Laboratorio de Insectos de Importancia Agron\u00f3mica IGEAF (INTA), GV\u2010IABIMO (CONICET)  Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5219-9582","authenticated-orcid":false,"given":"Santiago","family":"Revale","sequence":"additional","affiliation":[{"name":"Technology Department Alchemab Therapeutics Ltd.  London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Esteban","family":"Hasson","sequence":"additional","affiliation":[{"name":"Departamento de Ecolog\u00eda, Gen\u00e9tica y Evoluci\u00f3n, Facultad de Ciencias Exactas y Naturales Universidad de Buenos Aires (UBA), CABA  Buenos Aires Argentina"},{"name":"Instituto de Ecolog\u00eda, Gen\u00e9tica y Evoluci\u00f3n de Buenos Aires Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), CABA  Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,8,17]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btx431"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-019-0036-z"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.115.176669"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-40740-1_11"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1089\/106652700750050871"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btw686"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.089391.108"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.0060178"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/imb.12746"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkx976"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2119899119"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.RA118.000831"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200800795"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1198374"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1186\/1477-5956-4-9"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2010.09.002"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2020.0072"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/imb.12783"}],"container-title":["Insect Molecular Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/imb.12806","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/imb.12806","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/resjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/imb.12806","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T11:41:17Z","timestamp":1762083677000},"score":1,"resource":{"primary":{"URL":"https:\/\/resjournals.onlinelibrary.wiley.com\/doi\/10.1111\/imb.12806"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,8,17]]},"references-count":18,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2022,10]]}},"alternative-id":["10.1111\/imb.12806"],"URL":"https:\/\/doi.org\/10.1111\/imb.12806","archive":["Portico"],"relation":{},"ISSN":["0962-1075","1365-2583"],"issn-type":[{"value":"0962-1075","type":"print"},{"value":"1365-2583","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,8,17]]},"assertion":[{"value":"2022-06-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-07-25","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-08-17","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}