{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T16:32:25Z","timestamp":1791217945850,"version":"4.1.0"},"reference-count":18,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,9,9]],"date-time":"2022-09-09T00:00:00Z","timestamp":1662681600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002341","name":"Academy of Finland","doi-asserted-by":"publisher","award":["343022"],"award-info":[{"award-number":["343022"]}],"id":[{"id":"10.13039\/501100002341","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002341","name":"Academy of Finland","doi-asserted-by":"publisher","award":["328961"],"award-info":[{"award-number":["328961"]}],"id":[{"id":"10.13039\/501100002341","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004756","name":"Emil Aaltonen Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004756","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100018339","name":"Societas Biologica Fennica Vanamo","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100018339","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["resjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Insect Molecular Biology"],"published-print":{"date-parts":[[2023,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    CRISPR\u2010Cas9 has become one of the most prominent gene editing tools available and it has been utilized in various organisms from bacteria to fungi, plants, and animals. In this study, we developed a CRISPR\u2010Cas9 protocol for the black garden ant\n                    <jats:italic>Lasius niger<\/jats:italic>\n                    , a common and easily available study species for lab and field experiments. To create indel mutations using CRISPR\u2010Cas9 in\n                    <jats:italic>L. niger<\/jats:italic>\n                    , we targeted three different locations in a well\u2010studied eye pigmentation gene\n                    <jats:italic>cinnabar<\/jats:italic>\n                    , generating several mutations that disrupt the ommochrome biosynthesis pathway and result in the lack of the pigment and therefore, abnormal eye coloration in adult workers. We also developed a protocol to collect\n                    <jats:italic>L. niger<\/jats:italic>\n                    eggs, inject them with CRISPR\u2010Cas9 construct, and rear the eggs into mature adult workers with the assistance of nursing workers. We demonstrated for the first time in\n                    <jats:italic>L. niger<\/jats:italic>\n                    that CRISPR\u2010Cas9 is an excellent tool to create targeted mutations for this species. Our protocol can be referred to when developing similar studies for other species of ants and eusocial insects.\n                  <\/jats:p>","DOI":"10.1111\/imb.12809","type":"journal-article","created":{"date-parts":[[2022,8,28]],"date-time":"2022-08-28T09:40:26Z","timestamp":1661679626000},"page":"11-25","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Genetic modification of the ant\n                    <i>Lasius niger<\/i>\n                    using\n                    <scp>CRISPR\u2010Cas9<\/scp>\n                    technology"],"prefix":"10.1111","volume":"32","author":[{"given":"Mauno","family":"Konu","sequence":"first","affiliation":[{"name":"Ecology and Genetics Research Unit University of Oulu  Oulu Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonna","family":"Kulmuni","sequence":"additional","affiliation":[{"name":"Organismal and Evolutionary Research Programme University of Helsinki  Helsinki Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6587-6156","authenticated-orcid":false,"given":"Lumi","family":"Viljakainen","sequence":"additional","affiliation":[{"name":"Ecology and Genetics Research Unit University of Oulu  Oulu Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,9,9]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/22.1.76"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.2307\/3493193"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1101\/GR.1865504"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/ARCH.21527"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/S00040-020-00755-8"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/EEA.12530"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1534\/GENETICS.113.152710\/-\/DC1"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtherbio.2015.02.012"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1023\/B:JOEY.0000033808.45455.75"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.EXGER.2016.09.008"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-00990-3"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.GDE.2020.12.014"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.2842"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.3791\/61930"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2017.07.001"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1146\/ANNUREV-ECOLSYS-120213-091824"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.IBMB.2017.12.003"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.CELL.2017.06.051"}],"container-title":["Insect Molecular Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/imb.12809","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/imb.12809","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/resjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/imb.12809","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T10:32:13Z","timestamp":1762079533000},"score":1,"resource":{"primary":{"URL":"https:\/\/resjournals.onlinelibrary.wiley.com\/doi\/10.1111\/imb.12809"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,9,9]]},"references-count":18,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2023,2]]}},"alternative-id":["10.1111\/imb.12809"],"URL":"https:\/\/doi.org\/10.1111\/imb.12809","archive":["Portico"],"relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2022.05.02.490261","asserted-by":"object"}]},"ISSN":["0962-1075","1365-2583"],"issn-type":[{"value":"0962-1075","type":"print"},{"value":"1365-2583","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,9,9]]},"assertion":[{"value":"2022-05-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-08-22","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-09-09","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}