{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T04:16:19Z","timestamp":1784607379263,"version":"3.55.0"},"reference-count":57,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2022,12,9]],"date-time":"2022-12-09T00:00:00Z","timestamp":1670544000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004515","name":"Universiti Kebangsaan Malaysia","doi-asserted-by":"publisher","award":["GUP\u20102015\u2010010"],"award-info":[{"award-number":["GUP\u20102015\u2010010"]}],"id":[{"id":"10.13039\/501100004515","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["resjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Insect Molecular Biology"],"published-print":{"date-parts":[[2023,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    In Southeast Asia,\n                    <jats:italic>Conopomorpha cramerella<\/jats:italic>\n                    (Snellen) which is commonly known as the cocoa pod borer (CPB) moth has been identified as the most detrimental pest of\n                    <jats:italic>Theobroma cacao<\/jats:italic>\n                    L. Apart from the various side effects on human health and non\u2010target organisms, heavily relying on synthetic pyrethroid insecticides to control CPB infestations also increases the environmental contamination risks. Thus, developing biorational insecticides that minimally affect the non\u2010target organism and environment by targeting the insect growth regulation process is needed to manage the pest population. In insects, juvenile hormones (JH) regulate critical biological events, especially metamorphosis, development and reproduction. Since the physiological roles of JH III vary among different organisms, the biochemical properties, especially substrate specificity and analogue inhibition, may also be different. Therefore, studies on the JH III biosynthetic pathway enzymes in both plants and insects are beneficial to discover more effective analogues. Bioinformatic analysis and biochemical characterization of a NADP\n                    <jats:sup>+<\/jats:sup>\n                    \u2010dependent farnesol dehydrogenase, an intermediate enzyme of the JH III pathway, from\n                    <jats:italic>C.\u00a0cramerella<\/jats:italic>\n                    (CcFolDH), were described in this study. In addition, the farnesol analogues that may act as a potent analogue inhibitor for CcFolDH ware determined using in vitro enzymatic study. The phylogenetic analysis indicated that CcFolDH shared a close phylogenetic relationship to the honeybee's short\u2010chain dehydrogenase\/reductase. The 27\u2009kDa CcFolDH has an NADP(H) binding domain with a typical Rossmann fold and is likely a homotetrameric protein in the solution. The enzyme had a greater preference for substrate\n                    <jats:italic>trans<\/jats:italic>\n                    ,\n                    <jats:italic>trans<\/jats:italic>\n                    \u2010farnesol and coenzyme NADP\n                    <jats:sup>+<\/jats:sup>\n                    . In terms of analogue inhibitor inhibition, hexahydroxyfarnesyl acetone showed the highest inhibition (the lowest\n                    <jats:italic>K<\/jats:italic>\n                    <jats:sub>i<\/jats:sub>\n                    ) compared to other farnesol analogues. Thus, hexahydroxyfarnesyl acetone would serve as the most potent active ingredient for future biorational pesticide management for\n                    <jats:italic>C.\u00a0cramerella<\/jats:italic>\n                    infestation. Based on the bioinformatic analyses and biochemical characterizations conducted in this research, we proposed that rCcFolDH differs slightly from other reported farnesol dehydrogenases in terms of molecular weight, substrate preference, coenzymes utilization and analogue inhibitors selection.\n                  <\/jats:p>","DOI":"10.1111\/imb.12820","type":"journal-article","created":{"date-parts":[[2022,12,1]],"date-time":"2022-12-01T13:30:15Z","timestamp":1669901415000},"page":"143-159","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Isolation, purification and biochemical characterization of\n                    <i>Conopomorpha cramerella<\/i>\n                    farnesol dehydrogenase"],"prefix":"10.1111","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3174-4531","authenticated-orcid":false,"given":"Mahasakthy\u2010Vijeyasri","family":"Satyaveanthan","sequence":"first","affiliation":[{"name":"Institute of Systems Biology Universiti Kebangsaan Malaysia  Bangi Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chyan Leong","family":"Ng","sequence":"additional","affiliation":[{"name":"Institute of Systems Biology Universiti Kebangsaan Malaysia  Bangi Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alias","family":"Awang","sequence":"additional","affiliation":[{"name":"Cocoa Research &amp; Development Centre (Bagan Datuk) Malaysian Cocoa Board  Sg. Sumun Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kok Wai","family":"Lam","sequence":"additional","affiliation":[{"name":"Drug and Herbal Research Centre, Faculty of Pharmacy Universiti Kebangsaan Malaysia  Kuala Lumpur Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7322-2216","authenticated-orcid":false,"given":"Maizom","family":"Hassan","sequence":"additional","affiliation":[{"name":"Institute of Systems Biology Universiti Kebangsaan Malaysia  Bangi Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,12,9]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0143310"},{"key":"e_1_2_9_3_1","unstructured":"Bakar S.&Ismail A.(2007)Implementation of cocoa pod sleeving in controlling cocoa pod borer. 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