{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T13:39:19Z","timestamp":1781098759402,"version":"3.54.1"},"reference-count":64,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2022,12,28]],"date-time":"2022-12-28T00:00:00Z","timestamp":1672185600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["resjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Insect Molecular Biology"],"published-print":{"date-parts":[[2023,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Ecdysis triggering hormone (ETH) was originally discovered as a key hormone that regulates insect moulting via binding to its receptor, ETH receptor (ETHR). However, the precise role of ETH in moth reproduction remains to be explored in detail. ETH function was verified in vivo using\n                    <jats:italic>Mythimna separata<\/jats:italic>\n                    (Walker), an important cereal crop pest. RT\u2010qPCR analysis revealed that transcriptional expression profiles of\n                    <jats:italic>MsepETH<\/jats:italic>\n                    showed evident sexual dimorphism in the adult stage.\n                    <jats:italic>MsepETH<\/jats:italic>\n                    expression increased in the females on day 3 and persisted thereafter till day 7, consistent with female ovarian maturation, and was merely detectable in males. Meanwhile,\n                    <jats:italic>MsepETH<\/jats:italic>\n                    expression levels were significantly higher in the trachea than in other tissues.\n                    <jats:italic>MsepETHR\u2010A<\/jats:italic>\n                    and\n                    <jats:italic>MsepETHR\u2010B<\/jats:italic>\n                    were expressed in both sexes and were significantly higher in the antennae than in other tissues.\n                    <jats:italic>MsepETH<\/jats:italic>\n                    and\n                    <jats:italic>MsepETHR<\/jats:italic>\n                    knockdown in females by RNA interference significantly reduced the expression of\n                    <jats:italic>MsepETH<\/jats:italic>\n                    ,\n                    <jats:italic>MsepETHR\u2010A<\/jats:italic>\n                    ,\n                    <jats:italic>MsepETHR\u2010B<\/jats:italic>\n                    ,\n                    <jats:italic>MsepJHAMT<\/jats:italic>\n                    , and\n                    <jats:italic>MsepVG<\/jats:italic>\n                    , which delayed egg\u2010laying and significantly reduced egg production. RNAi 20\u2010hydroxyecdysone (20E) receptor (EcR) decreased\n                    <jats:italic>MsepETH<\/jats:italic>\n                    expression whereas injecting 20E restored egg production that had been disrupted by\n                    <jats:italic>MsepETH<\/jats:italic>\n                    interference. Meanwhile, RNAi juvenile hormone (JH) methoprene tolerant protein (Met) also decreased\n                    <jats:italic>MsepETH<\/jats:italic>\n                    expression and smearing JH analog methoprene (Meth) restored egg production. In conclusion, the reproduction roles of ETH, JH, and 20E were investigated in\n                    <jats:italic>M. separata<\/jats:italic>\n                    . These findings will lay the foundation for future research to develop an antagonist that reduces female reproduction and control strategies for pest insects.\n                  <\/jats:p>","DOI":"10.1111\/imb.12825","type":"journal-article","created":{"date-parts":[[2022,12,19]],"date-time":"2022-12-19T07:24:19Z","timestamp":1671434659000},"page":"213-227","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["The ecdysis triggering hormone system is essential for reproductive success in\n                    <i>Mythimna separata<\/i>\n                    (Walker)"],"prefix":"10.1111","volume":"32","author":[{"given":"Qi","family":"Yang","sequence":"first","affiliation":[{"name":"State Key Laboratory of Crop Stress Biology in Arid Areas, College of Plant Protection Northwest A&amp;F University  Yangling Shaanxi People's Republic of 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